实验原理：

凝胶迁移或电泳迁移率检测(Electrophoretic mobility shift assay，EMSA)是研究启动子结合蛋白的经典方法，主要应用于定性和定量分析核酸蛋白相互作用。

针对目标区域设计特异性核酸探针，并进行末端生物素标记；该探针与蛋白质结合形成复合物，电泳时在凝胶中移动的速度慢（相对于没有结合蛋白的探针），即表现为条带相对滞后。

该方法不仅简单迅速、灵敏度高，还可以用竞争性试验来评价蛋白和核酸结合的特性。目前用于检测DNA/RNA结合蛋白，并可通过加入特异性抗体来检测特定的蛋白质，或是结合蛋白双向电泳与质谱技术鉴定分析未知蛋白。

DNA-EMSA是一种研究目的蛋白与相关的DNA序列之间结合作用的技术。通过DNA-EMSA可以研究细胞内一些转录因子对特定基因的激活水平。本试剂盒提供了进行DNA-EMSA实验的主要试剂，包括蛋白提取、蛋白-DNA结合、电泳与化学发光等，使整体实验变得简单方便。DNA-EMSA/Gel-Shift结合体系中含有poly(dI-dC)等有效成分，其中，poly(dI-dC)的浓度经过优化，可以很好地消除蛋白和标记探针间的非特异性结合，同时又不会减弱目的转录因子和标记探针间的结合。

RNA-EMSA是一种研究RNA结合蛋白（RBPs）和特定RNA序列之间结合作用的技术。本试剂盒提供了进行RNA-EMSA实验的主要试剂，包括蛋白提取、蛋白-RNA结合、电泳与化学发光等，使整体实验变得简单方便。RNA-EMSA/Gel-Shift结合体系中含有poly(dI-dC)等有效成分，其中，poly(dI-dC)的浓度经过优化，可以很好地消除蛋白和标记探针间的非特异性结合，保证了实验检测的科学严谨性。此外，本试剂盒经去除RNA酶处理，一定程度上避免了实验过程中RNA降解。

产品优势：

1. 安全环保：采用非同位素的化学发光检测系统，免除放射性的危险与处理同位素废物的麻烦，减少环境污染；无需专门的同位素操作室，易于使用推广。

2. 高灵敏度：检测灵敏度高，背景干扰更低。

3. 快速省时：从标记探针到结果分析，全部实验只需5小时。

4. 稳定可靠：DNA末端标记生物素，不影响蛋白结合位点；标记好的DNA/RNA可以保存一年。

5. 配备完整：配备伯信生物细胞核提取试剂盒。

6. 个性化服务：可根据研究需要定制个性化高灵敏度探针。

技术流程：

详见产品说明书：Bes5003 DNA-EMSA  

详见产品说明书：Bes5107 RNA-EMSA 

文章案例

【2026年】

Article：Chondrocytes reprogram chromatin in hypoxic microenvironments to activate

Periodicals：Nature Communications

IF：15.7

 

Article：Iron overload reprogramming lipid metabolism through the

Periodicals：Experimental & Molecular Medicine

IF：12.9

【2025年】

Article：Integrated Transcriptomic and Proteomic Analyses Reveal CsrA-Mediated Regulation of Virulence and Metabolism in Vibrio alginolyticus. 

Periodicals：Microorganisms

IF：4.200

Article：PINX1 inhibits proliferation and cisplatin resistance in nasopharyngeal carcinoma by promoting ILF3 ubiquitination. 

Periodicals：American Journal of Cancer Research

IF：2.900

【2024年】

Article：Periodic changes of cyclin D1 mRNA stability are regulated by PC4 modifications in the cell cycle.

Periodicals：The Journal of Cell Biology

IF：7.400

【2023年】

Article：The RNA-binding protein hnRNP F is required for the germinal center B cell response

Periodicals：Nature Communications

IF：16.600

Article：An inducible long noncoding RNA, LncZFHX2, facilitates DNA repair to mediate osteoarthritis pathology

Periodicals：Redox Biology

IF：11.400

Article：DGW1, encoding an hnRNP-like RNA binding protein, positively regulates grain size and weight by interacting with GW6 mRNA. 

Periodicals：Plant biotechnology journal

IF：10.100

Article：OsCCRL1 is Essential for Phenylpropanoid Metabolism in Rice Anthers

Periodicals：Rice

IF：5.500

【2022年】

Article：ENKUR expression induced by chemically synthesized cinobufotalin suppresses malignant activities of hepatocellular carcinoma by modulating β-catenin/c-Jun/MYH9/USP7/c-Myc axis

Periodicals：International Journal of Biological Sciences

IF：9.200

Article：GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus

Periodicals：International Journal of Molecular Sciences

IF：5.600

【2021年】

Article：Global identification of full‐length cassava lncRNAs unveils the role of cold‐responsive intergenic lncRNA 1 in cold stress response

Periodicals：Plant Cell Environ

IF：7.300

Article：VPS33B suppresses lung adenocarcinoma metastasis and chemoresistance to cisplatin

Periodicals：Genes & Diseases

IF：7.103

Article：miR-1254 induced by NESG1 inactivates HDGF/DDX5-stimulated nuclear translocation of b-catenin and suppresses NPC metastasis

Periodicals：Molecular Therapy: Methods & Clinical Development

IF：6.698

Article：An insertion variant of MGMT disrupts a STAT1 binding site and confers susceptibility to glioma

Periodicals：Cancer Cell International

IF：5.722

Article：A direct negative feedback loop of miR-4721/FOXA1/Nanog promotes nasopharyngeal cell stem cell enrichment and metastasis

Periodicals：Journal of Translational Medicine

IF：5.531

Article：VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer

Periodicals：Cancer Science

IF：4.965

【2020年】

Article：Silencing MYH9 blocks HBx-induced GSK3β ubiquitination and degradation to inhibit tumor stemness in hepatocellular carcinoma

Periodicals：Signal Transduction and Targeted Therapy

IF：13.493

Article：MicroRNA-3613-5p Promotes Lung Adenocarcinoma Cell Proliferation through a RELA and AKT/MAPK Positive Feedback Loop

Periodicals：Molecular Therapy: Nucleic Acids

IF：8.889

Article：circRNA Hipk3 Induces Cardiac Regeneration after Myocardial Infarction in Mice by Binding to Notch1 and miR-133a

Periodicals：Molecular Therapy: Nucleic Acids

IF：8.889

Article：miR-4721, Induced by EBV-miR-BART22, Targets GSK3b to Enhance the Tumorigenic Capacity of NPC through the WNT/b-catenin Pathway

Periodicals：Molecular Therapy: Nucleic Acids

IF：8.886

Article：MicroRNA-4476 promotes glioma progression through a miR-4476/APC/β-catenin/c-Jun positive feedback loop

Periodicals：Cell Death & Disease

IF：8.469

Article：MiR-4310 induced by SP1 targets PTEN to promote glioma progression

Periodicals：Cancer Cell International

IF：4.178

【2019年】

Article：Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression

Periodicals：NATURE COMMUNICATIONS

IF：12.123

Article：Timeless-Stimulated miR-5188-FOXO1/b-Cateninc-Jun Feedback Loop Promotes Stemness via Ubiquitination of b-Catenin in Breast Cancer

Periodicals：Molecular Therapy

IF：11.455

Article：HBX-induced miR-5188 impairs FOXO1 to stimulate β-catenin nuclear translocation and promotes tumor stemness in hepatocellular carcinoma

Periodicals：Theranostics

IF：8.698

Article：VPS33B interacts with NESG1 to modulate EGFR/PI3K/AKT/c-Myc/P53/miR-133a-3p signaling and induce 5-fluorouracil sensitivity in nasopharyngeal carcinoma

Periodicals：Cell Death & Disease

IF：8.469

Article：Cinobufotalin powerfully reversed EBV-miR-BART22-induced cisplatin resistance via stimulating MAP2K4 to antagonize non-muscle myosin heavy chain IIA/glycogen synthase 3β/β-catenin signaling pathway

Periodicals：EBioMedicine

IF：5.736

Article：Genetic Predisposition to Glioma Mediated by a MAPKAP1 Enhancer Variant

Periodicals：Cellular and Molecular Neurobiology

IF：3.606

【2016年】

Article：miR-3188 regulates nasopharyngeal carcinoma proliferation and chemosensitivity through a FOXO1-modulated positive feedback loop with mTOR–p-PI3K/AKT-c-JUN

Periodicals：NATURE COMMUNICATIONS

IF：14.92