EMSA试剂盒
实验介绍

凝胶迁移或电泳迁移率检测(Electrophoretic mobility shift assay,EMSA)是研究启动子结合蛋白的经典方法,主要应用于定性和定量分析核酸蛋白相互作用。(本试剂盒仅供科研用途)

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详细介绍


实验原理:


凝胶迁移或电泳迁移率检测(Electrophoretic mobility shift assayEMSA)是研究启动子结合蛋白的经典方法,主要应用于定性和定量分析核酸蛋白相互作用。


针对目标区域设计特异性核酸探针,并进行末端生物素标记;该探针与蛋白质结合形成复合物,电泳时在凝胶中移动的速度慢(相对于没有结合蛋白的探针),即表现为条带相对滞后。


该方法不仅简单迅速、灵敏度高,还可以用竞争性试验来评价蛋白和核酸结合的特性。目前用于检测DNA/RNA结合蛋白,并可通过加入特异性抗体来检测特定的蛋白质,或是结合蛋白双向电泳与质谱技术鉴定分析未知蛋白。


DNA-EMSA是一种研究目的蛋白与相关的DNA序列之间结合作用的技术。通过DNA-EMSA可以研究细胞内一些转录因子对特定基因的激活水平。本试剂盒提供了进行DNA-EMSA实验的主要试剂,包括蛋白提取、蛋白-DNA结合、电泳与化学发光等,使整体实验变得简单方便。DNA-EMSA/Gel-Shift结合体系中含有poly(dI-dC)等有效成分,其中,poly(dI-dC)的浓度经过优化,可以很好地消除蛋白和标记探针间的非特异性结合,同时又不会减弱目的转录因子和标记探针间的结合。


RNA-EMSA是一种研究RNA结合蛋白(RBPs)和特定RNA序列之间结合作用的技术。本试剂盒提供了进行RNA-EMSA实验的主要试剂,包括蛋白提取、蛋白-RNA结合、电泳与化学发光等,使整体实验变得简单方便。RNA-EMSA/Gel-Shift结合体系中含有poly(dI-dC)等有效成分,其中,poly(dI-dC)的浓度经过优化,可以很好地消除蛋白和标记探针间的非特异性结合,保证了实验检测的科学严谨性。此外,本试剂盒经去除RNA酶处理,一定程度上避免了实验过程中RNA降解。


产品优势:


1. 安全环保:采用非同位素的化学发光检测系统,免除放射性的危险与处理同位素废物的麻烦,减少环境污染;无需专门的同位素操作室,易于使用推广。

2. 高灵敏度:检测灵敏度高,背景干扰更低。

3. 快速省时:从标记探针到结果分析,全部实验只需5小时。

4. 稳定可靠:DNA末端标记生物素,不影响蛋白结合位点;标记好的DNA/RNA可以保存一年。

5. 配备完整:配备伯信生物细胞核提取试剂盒。

6. 个性化服务:可根据研究需要定制个性化高灵敏度探针。



技术流程:










详见产品说明书Bes5003 DNA-EMSA  

详见产品说明书Bes5107 RNA-EMSA 



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