凝胶迁移或电泳迁移率检测(Electrophoretic mobility shift assay，EMSA)是研究启动子结合蛋白的经典方法，是一种用于定性和定量分析核酸蛋白相互作用的技术。该方法不仅简单迅速且灵敏度高；另一方面它可以用竞争性试验来评价蛋白和核酸结合的特性。目前用于检测DNA结合蛋白、RNA结合蛋白，并可通过加入特异性抗体来检测特定的蛋白质，结合蛋白双向电泳及质谱技术进行未知蛋白的鉴定分析。

针对目标区域设计特异性探针，并经过生物素标记。末端标记的核酸探针可以与蛋白质结合，电泳时探针与蛋白质复合物相对没有结合蛋白的探针在凝胶中移动的速度慢，即表现为相对滞后。

产品特点

1. 安全环保：采用非同位素的化学发光检测系统，免除放射性的危险与处理同位素废物的麻烦，减少环境污染；无须专门的同位素操作室，易于使用推广。

2. 高灵敏度：检测灵敏度高，等同于或者超过同位素标记系统，检测结果背景更低。

3. 快速省时：从标记探针到结果分析，全部实验只需5小时。

4. 稳定可靠：DNA末端标记生物素，不影响蛋白结合位点；标记好的DNA可以保存一年。

5. 配备完整：配备伯信生物细胞核提取试剂盒，个性化服务：可根据研究需要定制个性化高灵敏度探针。

技术流程

详见产品说明书：Bes5003 DNA-EMSA  

详见产品说明书：Bes5107 RNA-EMSA 

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