实验原理：

MeRIP利用m⁶A特异性抗体识别并结合mRNA上的m⁶A甲基化修饰位点，以RNA免疫共沉淀方法富集甲基化修饰片段，同时，结合高通量测序与qPCR，在全转录组范围内研究发生m⁶A甲基化修饰的RNA区域，及其对基因表达的调控作用。

 

产品优势：

1. 快速省时

2. 稳定可靠

3. 配备完整

技术流程：

示例结果：

详见产品说明书：Bes5203-1 m6A MeRIP  

详见产品说明书：Bes5203-2 m6A MeRIP（含m6A抗体） 

文章案例

【2025年】

Article：Methylation of GPRC5A promotes liver metastasis and docetaxel resistance through activating mTOR signaling pathway in triple negative breast cancer.

Periodicals：Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy

IF：15.800

Article：Betaine inhibits the stem cell-like properties of hepatocellular carcinoma by activating autophagy via SAM/m6A/YTHDF1-mediated enhancement on ATG3 stability. 

Periodicals：Theranostics

IF：12.400

Article：The m6A modification of SOX18 leads to increased PTX3 and cardiomyocyte pyroptosis in sepsis-induced cardiomyopathy. 

Periodicals：Theranostics

IF：12.400

Article：AGD1/USP10/METTL13 complexes enhance cancer stem cells proliferation and diminish the therapeutic effect of docetaxel via CD44 m6A modification in castration resistant prostate cancer.

Periodicals：Journal of experimental & clinical cancer research: CR

IF：11.400

Article： SRSF3 and hnRNP A1-mediated m6A-modified circCDK14 regulates intramuscular fat deposition by acting as miR-4492-z sponge.

Periodicals：Cellular & Molecular Biology Letters

IF：9.200

Article：YTHDC1 phase separation drives the nuclear export of m6A-modified lncNONMMUT062668.2 through the transport complex SRSF3-ALYREF-XPO5 to aggravate pulmonary fibrosis. 

Periodicals：Cell Death & Disease

IF：8.100

Article：METTL14-mediated m6A modification of ZFP14 inhibits clear cell renal cell carcinoma progression via promoting STAT3 ubiquitination. 

Periodicals：Clinical and Translational Medicine

IF：7.900

Article：N6-Methyladenosine modification mediated by METTL3 promotes DNA-PKcs expression to induce anlotinib resistance in osteosarcoma.

Periodicals：Clinical and Translational Medicine

IF：7.900

Article： An exploration on the involvement of the methyltransferase like 3-m6A‑zinc finger MYM-type containing 1 axis in the progression of liver hepatocellular carcinoma. 

Periodicals：International Journal of Biological Macromolecules

IF：7.700

Article：Helicobacter pylori reduces METTL14-mediated VAMP3 m6A modification and promotes the development of gastric cancer by regulating LC3C-mediated c-Met recycling.

Periodicals：Cell death discovery

IF：6.100

Article：MTHFD2 promotes esophageal squamous cell carcinoma progression via m6A modification‑mediated upregulation and modulation of the PEBP1‑RAF1 interaction.

Periodicals：International Journal of Molecular Medicine

IF：5.700

Article：Mettl3-Mediated N6-Methyladenosine Modification Mitigates Ganglion Cell Loss and Retinal Dysfunction in Retinal Ischemia-Reperfusion Injury by Inhibiting FoxO1-Mediated Autophagy.

Periodicals：Investigative Ophthalmology & Visual Scicence

IF：5.000

Article：GIV/Girdin Modulation of Microglial Activation in Ischemic Stroke: Impact of FTO-Mediated m6A Modification.

Periodicals：Molecular Neurobiology

IF：4.600

Article：Abnormal expression of CDC25C in NSCLC is influenced by transcriptional and RNA N6‑methyladenosine‑mediated post‑transcriptional regulation. 

Periodicals：International Journal of Oncology

IF：4.500

Article：AQP3 Influences Unexplained Recurrent Abortion by Regulating Trophoblast Cell Migration and Invasion via the METTL14/IGF2BP1/AQP3/PI3K/AKT Pathway.

Periodicals：Journal of cellular and molecular medicine

IF：4.300

Article：Wilms' tumor 1-associated protein aggravates ischemic stroke by promoting M1 polarization of microglia by enhancing PTGS2 mRNA stability in an m6A-dependent manner. 

Periodicals：Cell Biology International

IF：3.300

【2024年】

Article：Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway.

Periodicals：Molecular cancer

IF：27.700

Article：Natural product mediated mesenchymal-epithelial remodeling by covalently binding ENO1 to degrade m6A modified β-catenin mRNA.

Periodicals：Acta Pharmaceutica Sinica. B

IF：14.700

Article：circCDK13-loaded small extracellular vesicles accelerate healing in preclinical diabetic wound models. 

Periodicals：Nature communications

IF：14.700

Article：M6A-mediated upregulation of lncRNA TUG1 in liver cancer cells regulates the antitumor response of CD8+ T cells and phagocytosis of macrophages. 

Periodicals：Advanced Science

IF：14.300

Article：RNA modification gene WDR4 facilitates tumor progression and immunotherapy resistance in breast cancer. 

Periodicals：Journal of Advanced Research

IF：11.400

Article： FTO ameliorates doxorubicin-induced cardiotoxicity by inhibiting ferroptosis via P53-P21/Nrf2 activation in a HuR-dependent m6A manner.

Periodicals：Redox biology

IF：10.700

Article：Betaine alleviates cognitive impairment induced by homocysteine through attenuating NLRP3-mediated microglial pyroptosis in an m6A-YTHDF2-dependent manner. 

Periodicals：Redox biology

IF：10.700

Article：m6A-modified exosome-derived circHIF1α binding to KH domain of IGF2BP3 mediates DNA damage and arrests G1/S transition phase to resists bacterial infection in bacteremia.

Periodicals：Journal of Nanobiotechnology

IF：10.600

Article：FTO-mediated SMAD2 m6A modification protects cartilage against Osteoarthritis.

Periodicals：Experimental & Molecular Medicine

IF：9.500

Article：Noncoding RNA regulates the expression of Krm1 and Dkk2 to synergistically affect aortic valve lesions. 

Periodicals：Experimental & Molecular Medicine

IF：9.500

Article： Focused ultrasound combined with miR-1208-equipped exosomes inhibits malignant progression of glioma.

Periodicals：British Journal of Cancer

IF：6.400

Article：CircALMS1 Alleviates Pulmonary Microvascular Endothelial Cell Dysfunction in Pulmonary Hypertension. 

Periodicals：Journal of the American Heart Association

IF：5.000

Article：Glycolysis Induced by METTL14 Is Essential for Macrophage Phagocytosis and Phenotype in Cervical Cancer.

Periodicals：Journal of Immunology

IF：4.400

Article：Prognostic analysis of patients with gastric cancer based on N6-methyladenosine modification patterns and tumor microenvironment characterization. 

Periodicals：Frontiers in Pharmacology

IF：4.400

Article：ALKBH5-mediated upregulation of CPT1A promotes macrophage fatty acid metabolism and M2 macrophage polarization, facilitating malignant progression of colorectal cancer. 

Periodicals：Experimental Cell Research

IF：3.300

【2023年】

Article：FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke

Periodicals：Nature Communications

IF：17.694

Article：Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.

Periodicals：Nature Communications

IF：16.601

Article：METTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer

Periodicals：Cell Death and Disease

IF：9.696

Article：M6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy

Periodicals：International Journal of Biological Sciences

IF：9.200

Article：IGF2BP2-modified UBE2D1 interacts with Smad2/3 to promote the progression of breast cancer

Periodicals：American Journal of Cancer Research

IF：5.300

Article：F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

Periodicals：Cancers

IF：5.200

Article：RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

Periodicals：International Journal of Oncology

IF：5.200

Article：FTO Sensitizes Oral Squamous Cell Carcinoma to Ferroptosis via Suppressing ACSL3 and GPX4.

Periodicals：International Journal of Molecular Sciencices

IF：4.900

Article：METTL3 exacerbates insulin resistance in hepatocytes by regulating m6A modification of cytochrome P450 2B6

Periodicals：Nutrition & Metabolism

IF：4.500

Article：METTL3-regulated m6A modification impairs the decidualization of endometrial stromal cells by regulating YTHDF2-mediated degradation of FOXO1 mRNA in endometriosis-related infertility.

Periodicals：Reproductive biology and endocrinology

IF：4.400

Article：FLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

Periodicals：Heliyon

IF：4.000

【2022年】

Article：Silencing of IRF8 Mediated by m6A Modification Promotes the Progression of T-Cell Acute Lymphoblastic Leukemia

Periodicals：Advanced Science

IF：17.521

Article：METTL3 mediates chemoresistance by enhancing AML homing and engraftment via ITGA4

Periodicals：Leukemia

IF：12.883

Article：ALKBH5-mediated m6A modifcation of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：12.658

Article：AKAP8L enhances the stemness and chemoresistance of gastric cancer cells by stabilizing SCD1 mRNA

Periodicals：Cell Death and Disease

IF：9.685

Article：M6A demethylase fat mass and obesity-associated protein regulates cisplatin resistance of gastric cancer by modulating autophagy activation through ULK1

Periodicals：Cancer Science

IF：6.518

Article：The m6A Methyltransferase METTL3 Ameliorates Methylglyoxal-Induced Impairment of Insulin Secretion in Pancreatic b Cells by Regulating MafA Expression

Periodicals：Frontiers in Endocrinology

IF：6.055

Article：The m6A Methyltransferase METTL3-Mediated N6-Methyladenosine Modification of DEK mRNA to Promote Gastric Cancer Cell Growth and Metastasis

Periodicals：International Journal of Molecular Sciences

IF：5.924

Article：METTL14-mediated N6-methyladenosine modifcation of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma

Periodicals：Cell Communication and Signaling

IF：5.712

Article：N6-methyladenosine demethylase FTO suppressed prostate cancer progression by maintaining CLIC4 mRNA stability

Periodicals：Cell Death Discovery

IF：5.24

Article：Methyltransferase-like 3 modulates visceral hypersensitivity through regulating the nuclear export of circKcnk9 in YTHDC1-dependent manner

Periodicals：Molecular Pain

IF：3.37

【2021年】

Article：Saikosaponin D exhibits anti-leukemic activity by targeting FTO/m6A signaling

Periodicals：Theranostics

IF：8.578

Article：Low Expression of YTH Domain-Containing 1 Promotes Microglial M1 Polarization by Reducing the Stability of Sirtuin 1 mRNA

Periodicals：Frontiers in Cellular Neuroscience

IF：5.505

【2019年】

Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

Periodicals：Advanced Science

IF：17.521