实验原理：

MeRIP利用m⁶A特异性抗体识别并结合mRNA上的m⁶A甲基化修饰位点，以RNA免疫共沉淀方法富集甲基化修饰片段，同时，结合高通量测序与qPCR，在全转录组范围内研究发生m⁶A甲基化修饰的RNA区域，及其对基因表达的调控作用。

 

产品优势：

1. 快速省时

2. 稳定可靠

3. 配备完整

技术流程：

详见产品说明书：Bes5203-1 m6A MeRIP  

详见产品说明书：Bes5203-2 m6A MeRIP（含m6A抗体） 

文章案例

【2024年】

Article：Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway.

Periodicals：Molecular cancer

IF：27.700

Article：circCDK13-loaded small extracellular vesicles accelerate healing in preclinical diabetic wound models. 

Periodicals：Nature communications

IF：14.700

Article：M6A-mediated upregulation of lncRNA TUG1 in liver cancer cells regulates the antitumor response of CD8+ T cells and phagocytosis of macrophages. 

Periodicals：Advanced Science

IF：14.300

Article： FTO ameliorates doxorubicin-induced cardiotoxicity by inhibiting ferroptosis via P53-P21/Nrf2 activation in a HuR-dependent m6A manner.

Periodicals：Redox biology

IF：11.400

Article：Betaine alleviates cognitive impairment induced by homocysteine through attenuating NLRP3-mediated microglial pyroptosis in an m6A-YTHDF2-dependent manner. 

Periodicals：Redox biology

IF：11.400

Article：Noncoding RNA regulates the expression of Krm1 and Dkk2 to synergistically affect aortic valve lesions. 

Periodicals：Experimental & Molecular Medicine

IF：9.500

Article：Fumarate induces LncRNA-MIR4435-2HG to regulate glutamine metabolism remodeling and promote the development of FH-deficient renal cell carcinoma.

Periodicals：Cell death & Disease

IF：8.100

Article： Focused ultrasound combined with miR-1208-equipped exosomes inhibits malignant progression of glioma.

Periodicals：British Journal of Cancer

IF：6.400

Article：CircALMS1 Alleviates Pulmonary Microvascular Endothelial Cell Dysfunction in Pulmonary Hypertension. 

Periodicals：Journal of the American Heart Association

IF：5.000

Article：Glycolysis Induced by METTL14 Is Essential for Macrophage Phagocytosis and Phenotype in Cervical Cancer.

Periodicals：Journal of Immunology

IF：4.400

Article：Prognostic analysis of patients with gastric cancer based on N6-methyladenosine modification patterns and tumor microenvironment characterization. 

Periodicals：Frontiers in Pharmacology

IF：4.400

【2023年】

Article：FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke

Periodicals：Nature Communications

IF：17.694

Article：Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.

Periodicals：Nature Communications

IF：16.601

Article：METTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer

Periodicals：Cell Death and Disease

IF：9.696

Article：M6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy

Periodicals：International Journal of Biological Sciences

IF：9.200

Article：IGF2BP2-modified UBE2D1 interacts with Smad2/3 to promote the progression of breast cancer

Periodicals：American Journal of Cancer Research

IF：5.300

Article：F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

Periodicals：Cancers

IF：5.200

Article：RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

Periodicals：International Journal of Oncology

IF：5.200

Article：FTO Sensitizes Oral Squamous Cell Carcinoma to Ferroptosis via Suppressing ACSL3 and GPX4.

Periodicals：International Journal of Molecular Sciencices

IF：4.900

Article：METTL3 exacerbates insulin resistance in hepatocytes by regulating m6A modification of cytochrome P450 2B6

Periodicals：Nutrition & Metabolism

IF：4.500

Article：METTL3-regulated m6A modification impairs the decidualization of endometrial stromal cells by regulating YTHDF2-mediated degradation of FOXO1 mRNA in endometriosis-related infertility.

Periodicals：Reproductive biology and endocrinology

IF：4.400

Article：FLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

Periodicals：Heliyon

IF：4.000

【2022年】

Article：Silencing of IRF8 Mediated by m6A Modification Promotes the Progression of T-Cell Acute Lymphoblastic Leukemia

Periodicals：Advanced Science

IF：17.521

Article：METTL3 mediates chemoresistance by enhancing AML homing and engraftment via ITGA4

Periodicals：Leukemia

IF：12.883

Article：ALKBH5-mediated m6A modifcation of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：12.658

Article：AKAP8L enhances the stemness and chemoresistance of gastric cancer cells by stabilizing SCD1 mRNA

Periodicals：Cell Death and Disease

IF：9.685

Article：M6A demethylase fat mass and obesity-associated protein regulates cisplatin resistance of gastric cancer by modulating autophagy activation through ULK1

Periodicals：Cancer Science

IF：6.518

Article：The m6A Methyltransferase METTL3 Ameliorates Methylglyoxal-Induced Impairment of Insulin Secretion in Pancreatic b Cells by Regulating MafA Expression

Periodicals：Frontiers in Endocrinology

IF：6.055

Article：The m6A Methyltransferase METTL3-Mediated N6-Methyladenosine Modification of DEK mRNA to Promote Gastric Cancer Cell Growth and Metastasis

Periodicals：International Journal of Molecular Sciences

IF：5.924

Article：METTL14-mediated N6-methyladenosine modifcation of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma

Periodicals：Cell Communication and Signaling

IF：5.712

Article：N6-methyladenosine demethylase FTO suppressed prostate cancer progression by maintaining CLIC4 mRNA stability

Periodicals：Cell Death Discovery

IF：5.24

Article：Methyltransferase-like 3 modulates visceral hypersensitivity through regulating the nuclear export of circKcnk9 in YTHDC1-dependent manner

Periodicals：Molecular Pain

IF：3.37

【2021年】

Article：Saikosaponin D exhibits anti-leukemic activity by targeting FTO/m6A signaling

Periodicals：Theranostics

IF：8.578

Article：Low Expression of YTH Domain-Containing 1 Promotes Microglial M1 Polarization by Reducing the Stability of Sirtuin 1 mRNA

Periodicals：Frontiers in Cellular Neuroscience

IF：5.505

【2019年】

Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

Periodicals：Advanced Science

IF：17.521