m6A MeRIP试剂盒
实验介绍

RNA甲基化指发生在RNA分子上不同位置的甲基化修饰现象,属于转录后修饰,常见的甲基化修饰方式有6-甲基腺嘌呤(N6-methyladenosine,m6A)和5-甲基胞嘧啶(C5-methylcytidine,m5C)以及7-甲基鸟嘌呤(N7-methylguanosine,m7G)等。RNA甲基化在调控基因表达、剪接、RNA编辑、RNA稳定性、控制mRNA寿命和降解、介导环状RNA翻译等方面可能扮演重要角色。利用甲基化RNA免疫共沉淀(Methylated RNA Immunoprecipitation,MeRIP)技术,可以对RNA转录后甲基化修饰图谱进行全面研究。 (本试剂盒仅供科研用途)

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详细介绍


实验原理:


MeRIP利用m6A特异性抗体识别并结合mRNA上的m6A甲基化修饰位点,以RNA免疫共沉淀方法富集甲基化修饰片段,同时,结合高通量测序与qPCR,在全转录组范围内研究发生m6A甲基化修饰的RNA区域,及其对基因表达的调控作用。

 

产品优势:


1. 快速省时

2. 稳定可靠

3. 配备完整


技术流程:




详见产品说明书Bes5203-1 m6A MeRIP  

详见产品说明书Bes5203-2 m6A MeRIP(含m6A抗体) 




文章案例



2023


ArticleFTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke

PeriodicalsNature Communications

IF17.694


ArticleCircular RNA encoded MET variant promotes glioblastoma tumorigenesis.

PeriodicalsNature Communications

IF16.601


ArticleMETTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer

PeriodicalsCell Death and Disease

IF9.696


ArticleM6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy

PeriodicalsInternational Journal of Biological Sciences

IF:9.200


ArticleIGF2BP2-modified UBE2D1 interacts with Smad2/3 to promote the progression of breast cancer

Periodicals:American Journal of Cancer Research

IF:5.300


ArticleF-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

PeriodicalsCancers

IF:5.200


ArticleRBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

Periodicals:International Journal of Oncology

IF:5.200


ArticleMETTL3 exacerbates insulin resistance in hepatocytes by regulating m6A modification of cytochrome P450 2B6

Periodicals:Nutrition & Metabolism

IF:4.500


ArticleMETTL3-regulated m6A modification impairs the decidualization of endometrial stromal cells by regulating YTHDF2-mediated degradation of FOXO1 mRNA in endometriosis-related infertility.

PeriodicalsReproductive biology and endocrinology

IF4.400


ArticleFLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

Periodicals:Heliyon

IF4.000



2022


ArticleSilencing of IRF8 Mediated by m6A Modification Promotes the Progression of T-Cell Acute Lymphoblastic Leukemia

PeriodicalsAdvanced Science

IF17.521


ArticleMETTL3 mediates chemoresistance by enhancing AML homing and engraftment via ITGA4

PeriodicalsLeukemia

IF12.883


ArticleALKBH5-mediated m6A modifcation of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription

PeriodicalsJournal of Experimental & Clinical Cancer Research

IF12.658


ArticleAKAP8L enhances the stemness and chemoresistance of gastric cancer cells by stabilizing SCD1 mRNA

PeriodicalsCell Death and Disease

IF9.685


ArticleM6A demethylase fat mass and obesity-associated protein regulates cisplatin resistance of gastric cancer by modulating autophagy activation through ULK1

PeriodicalsCancer Science

IF6.518


ArticleThe m6A Methyltransferase METTL3 Ameliorates Methylglyoxal-Induced Impairment of Insulin Secretion in Pancreatic b Cells by Regulating MafA Expression

PeriodicalsFrontiers in Endocrinology

IF6.055


ArticleThe m6A Methyltransferase METTL3-Mediated N6-Methyladenosine Modification of DEK mRNA to Promote Gastric Cancer Cell Growth and Metastasis

PeriodicalsInternational Journal of Molecular Sciences

IF5.924


ArticleMETTL14-mediated N6-methyladenosine modifcation of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma

PeriodicalsCell Communication and Signaling

IF5.712


ArticleN6-methyladenosine demethylase FTO suppressed prostate cancer progression by maintaining CLIC4 mRNA stability

PeriodicalsCell Death Discovery

IF5.24


ArticleMethyltransferase-like 3 modulates visceral hypersensitivity through regulating the nuclear export of circKcnk9 in YTHDC1-dependent manner

PeriodicalsMolecular Pain

IF3.37



2021


ArticleSaikosaponin D exhibits anti-leukemic activity by targeting FTO/m6A signaling

PeriodicalsTheranostics

IF8.578


ArticleLow Expression of YTH Domain-Containing 1 Promotes Microglial M1 Polarization by Reducing the Stability of Sirtuin 1 mRNA

PeriodicalsFrontiers in Cellular Neuroscience

IF5.505



2019


Article:Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

PeriodicalsAdvanced Science

IF17.521

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