实验原理：

MeRIP利用m⁶A特异性抗体识别并结合mRNA上的m⁶A甲基化修饰位点，以RNA免疫共沉淀方法富集甲基化修饰片段，同时，结合高通量测序与qPCR，在全转录组范围内研究发生m⁶A甲基化修饰的RNA区域，及其对基因表达的调控作用。

 

产品优势：

1. 快速省时

2. 稳定可靠

3. 配备完整

技术流程：

详见产品说明书：Bes5203-1 m6A MeRIP  

详见产品说明书：Bes5203-2 m6A MeRIP（含m6A抗体） 

文章案例

【2023年】

Article：FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke

Periodicals：Nature Communications

IF：17.694

Article：Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.

Periodicals：Nature Communications

IF：16.601

Article：M6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy

Periodicals：International Journal of Biological Sciences

IF：10.75

Article：METTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer

Periodicals：Cell Death and Disease

IF：9.696

Article：F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

Periodicals：Cancers

IF：6.575

Article：IGF2BP2-modified UBE2D1 interacts with Smad2/3 to promote the progression of breast cancer

Periodicals：American Journal of Cancer Research

IF：5.300

Article：FLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

Periodicals：Heliyon

IF：3.776

【2022年】

Article：Silencing of IRF8 Mediated by m6A Modification Promotes the Progression of T-Cell Acute Lymphoblastic Leukemia

Periodicals：Advanced Science

IF：17.521

Article：METTL3 mediates chemoresistance by enhancing AML homing and engraftment via ITGA4

Periodicals：Leukemia

IF：12.883

Article：ALKBH5-mediated m6A modifcation of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：12.658

Article：AKAP8L enhances the stemness and chemoresistance of gastric cancer cells by stabilizing SCD1 mRNA

Periodicals：Cell Death and Disease

IF：9.685

Article：M6A demethylase fat mass and obesity-associated protein regulates cisplatin resistance of gastric cancer by modulating autophagy activation through ULK1

Periodicals：Cancer Science

IF：6.518

Article：The m6A Methyltransferase METTL3 Ameliorates Methylglyoxal-Induced Impairment of Insulin Secretion in Pancreatic b Cells by Regulating MafA Expression

Periodicals：Frontiers in Endocrinology

IF：6.055

Article：The m6A Methyltransferase METTL3-Mediated N6-Methyladenosine Modification of DEK mRNA to Promote Gastric Cancer Cell Growth and Metastasis

Periodicals：International Journal of Molecular Sciences

IF：5.924

Article：METTL14-mediated N6-methyladenosine modifcation of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma

Periodicals：Cell Communication and Signaling

IF：5.712

Article：N6-methyladenosine demethylase FTO suppressed prostate cancer progression by maintaining CLIC4 mRNA stability

Periodicals：Cell Death Discovery

IF：5.24

Article：Methyltransferase-like 3 modulates visceral hypersensitivity through regulating the nuclear export of circKcnk9 in YTHDC1-dependent manner

Periodicals：Molecular Pain

IF：3.37

【2021年】

Article：Saikosaponin D exhibits anti-leukemic activity by targeting FTO/m6A signaling

Periodicals：Theranostics

IF：8.578

Article：Low Expression of YTH Domain-Containing 1 Promotes Microglial M1 Polarization by Reducing the Stability of Sirtuin 1 mRNA

Periodicals：Frontiers in Cellular Neuroscience

IF：5.505

【2019年】

Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

Periodicals：Advanced Science

IF：17.521