DNA pulldown试剂盒
实验介绍

DNA pulldown是一种用于研究DNA与蛋白质互作的技术。通常用来分析目标基因调控区域相结合的转录因子及其他调控蛋白。 (本试剂盒仅供科研用途)

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详细介绍


实验原理:


针对目标区域设计生物素标记的特异性DNA探针偶联磁珠,并与细胞核提取物孵育。蛋白分子与DNA探针特异性结合,通过洗脱,得到目的DNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。

 

产品优势:


1.自主品牌:拥有自主知识产权专利。

2.快速省时:从标记探针到结果分析,全部实验只需3小时。

3.稳定可靠:DNA末端标记生物素,不影响蛋白结合位点。标记好的DNA可以保存一年。

4.配备完整:该试剂盒包含对照系统,只需要自备生物素末端标记的DNA探针及核蛋白质样品。

5.可得到完整、高浓度蛋白,用于质谱检测。




技术流程:



详见产品说明书Bes5004 DNA pulldown



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