环状RNA(circRNA)过表达载体
实验介绍

伯信生物利用专利技术,成功研发出特异性准确过表达 circRNAs 的表达载体,率先为研究者提供简便高效的 circRNAs 过表达研究策略。现已推出2种 circRNAs 表达载体,适用于普通真核表达,慢病毒包装,绿色荧光和嘌呤霉素筛选等多种用途。该载体含有伯信生物专利技术的 circRNAs 环化表达框架,载体转染细胞后可通过 RNA 剪接形成 circRNAs 分子。研究者仅需以 PCR 扩增目标 circRNAs 的线性序列,克隆进表达载体中即可,大大简化实验步骤,节约时间。 (仅供科研用途)

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详细介绍

环状RNACircular RNAs是广泛且多样地存在于多种生物细胞中,具有调控基因表达作用的一类内源性ncRNA分子。目前的研究中,仅仅明确了circRNA具有吸附miRNA的海绵作用、调控基因转录等作用。但是想要进一步明确circRNA在生物体内的形成机制及发挥的功能仍需要更多的circRNA相关数据来佐证。因此,circRNA过表达载体在针对circRNA功能机制的研究中起到必不可少的工具性作用。


      伯信生物利用专利技术,成功研发出特异性准确过表达 circRNAs 的表达载体,率先为研究者提供简便高效的 circRNAs 过表达研究策略。现已推出2种 circRNAs 表达载体,适用于普通真核表达,慢病毒包装,绿色荧光和嘌呤霉素筛选等多种用途。该载体含有伯信生物专利技术的 circRNAs 环化表达框架,载体转染细胞后可通过 RNA 接形成 circRNAs 分子。研究者仅需以 PCR 扩增目标 circRNAs 的线性序列,克隆进表达载体中即可,大大简化实验步骤,节约时间。






升级版circRNA过表达载体新登场!




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                    *升级版circRNA过表达载体(质粒、慢病毒),过表达效率高达1000倍以上!环化效率高达95%!
1)过表达效率高——circRNAs 表达载体上加有伯信生物专利技术的上下游环化框架,验证过的所有 circRNAs 都能显著过表达;

2)序列环化准确——环化框架内部引入伯信生物专利技术的环化介导序列,能有效保证目标 circRNAs 的线性序列准确环化,验证过的所有 circRNAs 都能准确环化,无碱基添加或缺失现象;
3)过表达稳定性高——验证过的目标 circRNAs 从 200bp 到 2500bp 序列都能实现准确高效过表达;
4)适用范围广——适用于普通真核表达,慢病毒包装,绿色荧光和嘌呤霉素筛选等多种用途





文章案例


ArticleTTBK2 circular RNA promotes glioma malignancy by regulating miR-217/HNF1β/Derlin-1 pathway

PeriodicalsJournal of Hematology & Oncology

IF17.388


ArticleCircECE1 activates energy metabolism in osteosarcoma by stabilizing c-Myc

PeriodicalsMolecular Cancer

IF15.302


ArticleCircSERPINE2 protects against osteoarthritis by targeting miR-1271 and ETS-related gene

PeriodicalsAnnals of the Rheumatic Diseases

IF14.299


ArticleCircular RNA hsa_circ_0110389 promotes gastric cancer progression through upregulating SORT1 via sponging miR-127-5p and miR-136-5p

PeriodicalsCell Death & Disease

IF8.469


ArticleCirc_2858 helps blood-brain barrier disruption by increasing VEGFA via sponging miR-93-5p during Escherichia coli meningitis

PeriodicalsMolecular Therapy: Nucleic Acid

IF7.032


ArticleCircular RNA transcriptomics analysis of primary human brain microvascular endothelial cells infected with meningitic Escherichia coli

PeriodicalsMolecular Therapy: Nucleic Acid

IF7.032


ArticleCircular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis

PeriodicalsCell Biology and Toxicology

IF6.69


ArticleSuper-Enhancer LncRNA LINC00162 Promotes Progression of Bladder Cancer

PeriodicalsiScience

IF4.447


ArticleCircLMNB1 promotes colorectal cancer by regulating cell proliferation, apoptosis and epithelial-mesenchymal transition

PeriodicalsOncoTargets and Therapy

IF3.337


ArticleCircRNA_0044556 diminishes the sensitivity of triplenegative breast cancer cells to adriamycin by sponging miR145 and regulating NRAS

PeriodicalsMolecular Medicine REPORTS

IF2.95


Article:CircLMTK2 acts as a novel tumor suppressor in gastric cancer

Periodicals:Bioscience Reports

IF:2.942


ArticleCircRNA hsa_circ_0005909 Promotes Cell Proliferation of Osteosarcoma Cells by Targeting miR-338-3p/HMGA1 Axis

PeriodicalsCancer Management and Research

IF2.886


ArticleCirc_0084927 Facilitates Cervical Cancer Development via Sponging miR-142-3p and Upregulating ARL2

PeriodicalsCancer Management and Research

IF2.886


ArticleCircular RNA CircCDYL Regulates Proliferation and Apoptosis in Non-Small Cell Lung Cancer Cells by Sponging miR-185-5p and Upregulating TNRC6A

PeriodicalsCancer Management and Research

IF2.886


ArticleCircular RNA Circ-ZNF609 Promotes Lung Adenocarcinoma Proliferation by Modulating miR-1224-3p/ETV1 Signaling

PeriodicalsCancer Management and Research

IF2.886