伯信生物建立了荧光原位杂交探针（FISH Probe）制备平台，承接荧光原位杂交试剂盒定制业务，可根据客户需求进行个性化定制。

该试剂盒主要用于检测样本中目的基因的表达量与表达部位，可应用于组织切片（石蜡切片/冰冻切片）、细胞爬片与染色体片等的检测。

伯信生物采用从研究物种自体获取特异性探针并进行化学共价方式与荧光素连接。

自体荧光探针具有明显的优越性：灵敏度高，光稳定性好，荧光寿命长；最大的优势就是自体探针可以有效的降低自体的非特异荧光干扰，达到更加高效特异的基因检测信号。

通过对比将会产生极大的技术效应，获取客户的认可并替代传统的探针检测，满足国内外科研工作者对高质量的荧光探针原位杂交试剂盒的实验需求。

伯信生物荧光原位杂交试剂盒，自上市以来深受广大科研用户的欢迎和肯定，为中国科学院、中山大学医学院及附属医院、南方医科大学医学院及附属医院、香港大学、北京大学医学部及附属医院、东南大学医学院、江西农业大学、华中科技大学、同济医学院等国内一流科研院所、医疗机构及跨国生物企业等近千家用户提供了优质的服务。

同时经过多年的积累，2011年建立了分子探针研发中心，伯信生物拥有强大的荧光原位杂交技术团队，为客户在试剂盒使用中遇到的各种问题给予技术支持和解答。

目前公司拥有库存现货试剂盒种类已达近万种：其中mRNA 1245种；microRNA 3171种，种属涵盖人、大小鼠、兔、猪、鸡、细菌、昆虫类等等，以及其他常规染色体探针及种属特异性探针等。

实验原理：

荧光原位杂交（Fluorescent in situ hybridization, FISH）是根据待测核酸序列设计特异性的荧光素标记寡聚核苷酸探针，再经过共变性-退火-复性，使探针与靶DNA/RNA按照碱基互补配对原则形成杂交体，最后利用荧光显微镜直接检测荧光信号，从而对组织、细胞中的待测核酸进行定性、半定量或相对定位分析的一种原位杂交技术。

技术流程：

产品优势：

1. 探针制备采用直接标记法，省去了信号放大系统。

2. 荧光信号强，结果判定直观可靠。

3. 根据客户需求定制个性化探针。

4. 特异性强，灵敏度高，背景低。

5. 探针性能稳定，低温保存一年以上。

6. 同时示踪核酸和蛋白的定位情况。

7. 操作简单，安全、快速，重复性好。

详见产品说明书：Bes1001 RNA-FISH（短链）

详见产品说明书：Bes1002 RNA-FISH（长链） 

详见产品说明书：Bes1011 DNA-FISH

文章案例

【2026年】

Article：LncRNA SBF2-AS1 Regulates Pyroptosis to Promote Chlamydia trachomatis Growth Through miR-196b-5p/RIPK2 Axis
Periodicals：Microorganisms
IF：4.1998

 

Article：LINC02878/ZNF282/PYCR2 axis promotes proline synthesis and tumor progression in colorectal cancer
Periodicals：Cellular and Molecular Life Sciences
IF：6.2001

【2025年】

Article：Chromatin dynamics of a large-sized genome provides insights into polyphenism and X0 dosage compensation of locusts.（DNA-FISH）

Periodicals：Nature Genetics

IF：29.000

Article：Lung metastasis and recurrence is mitigated by CAR macrophages, in-situ-generated from mRNA delivered by small extracellular vesicles.（DNA-FISH）

Periodicals：Nature Communications

IF：15.700

Article：Streamlined metal-based hydrogel facilitates stem cell differentiation, extracellular matrix homeostasis and cartilage repair in male rats.

Periodicals：Nature Communications

IF：15.700

Article： Statin therapy associated Lactobacillus intestinalis attenuates pancreatic fibrosis through remodeling intestinal homeostasis. NPJ Biofilms Microbiomes.

Periodicals：NPJ Biofilms and Microbiomes

IF：7.800

Article：TEC-mediated tRF-31R9J regulates histone lactylation and acetylation by HDAC1 to suppress hepatocyte ferroptosis and improve non-alcoholic steatohepatitis.

Periodicals：Clinical Epigenetics

IF：4.800

Article： Erianin ameliorates morphine tolerance and glioma progression through the JAK2-STAT3 pathway.

Periodicals：Translational Oncology

IF：4.100

Article：Qufeng epimedium decoction alleviates rheumatoid arthritis through CYLD-antagonized NF-kB activation by deubiquitinating Sirt1. 

Periodicals：Immunobiology

IF：2.500

【2024年】

Article：LncRNA-Mediated TPI1 and PKM2 Promote Self-Renewal and Chemoresistance in GBM. 

Periodicals：Advanced science (Weinheim, Baden-Wurttemberg, Germany)

IF：14.300

Article：EIF4A3 modulated circ_000999 promotes epithelial-mesenchymal transition in cadmium-induced malignant transformation through the miR-205-5p/ZEB1 axis.

Periodicals：Environment International

IF：10.300

Article：Periodic changes of cyclin D1 mRNA stability are regulated by PC4 modifications in the cell cycle.

Periodicals：The Journal of Cell Biology

IF：7.400

Article：MiR-4769-3p suppresses adipogenesis in systemic sclerosis by negatively regulating the USP18/VDAC2 pathway.

Periodicals：iScience

IF：7.100

Article：circ-1584 selectively promotes the antitumor activity of the oncolytic virus M1 on pancreatic cancer.

Periodicals：Molecular Therapy. Oncology

IF：5.299

Article：Hsa_circRNA_000166 accelerates breast cancer progression via the regulation of the miR-326/ELK1 and miR-330-5p/ELK1 axes. 

Periodicals：Annals of Medicine

IF：4.900

Article：Sws2 Gene Positively Regulates Melanin Production in Plectropomus leopardus Skin via Direct Regulation of the Synthesis of Retinoic Acid.

Periodicals：International Journal of Molecular Sciences

IF：4.900

Article： MiR-4769-3p suppresses adipogenesis in systemic sclerosis by negatively regulating the USP18/VDAC2 pathway.(miRNA)

Periodicals：iScience

IF：4.600

Article：The IGF2BP2-lncRNA TRPC7-AS1 axis promotes hepatocellular carcinoma cell proliferation and invasion.

Periodicals：Cellular Signalling

IF：4.400

Article： LncRNA HOTTIP promotes LPS-induced lung epithelial cell injury by recruiting DNMT1 to epigenetically regulate SP-C.

Periodicals：Journal of cell communication and signaling

IF：4.100

Article：Long non‑coding RNA DANCR aggravates breast cancer through the miR‑34c/E2F1 feedback loop. 

Periodicals：Molecular medicine reports

IF：3.400

Article：lncRNA H19 facilitates vascular neointima formation by targeting miR-125a-3p/FLT1 axis.

Periodicals：Acta biochimica et biophysica Sinica

IF：3.300

Article：LncIRF1 promotes chicken resistance to ALV-J infection.

Periodicals：3 Biotech

IF：2.600

【2023年】

Article：circEIF3I facilitates the recruitment of SMAD3 to early endosomes to promote TGF-β signalling pathway-mediated activation of MMPs in pancreatic cancer

Periodicals：Molecular Cancer

IF：37.299

Article：Augmenting L3MBTL2-induced condensates suppresses tumor growth in osteosarcoma. 

Periodicals：Science Advances

IF：13.600

Article：CircFOXK2 promotes hepatocellular carcinoma progression and leads to a poor clinical prognosis via regulating the Warburg efect

Periodicals：Journal of Experimental &Clinical Cancer Research

IF：12.658

Article：N6-methyladenosine-modified circRIMS2 mediates synaptic and memory impairments by activating GluN2B ubiquitination in Alzheimer's disease.

Periodicals：Translational Neurodegeneration

IF：12.600

Article：Hsa_circ_0009092/miR-665/NLK signaling axis suppresses colorectal cancer progression via recruiting TAMs in the tumor microenvironment.（miRNA）

Periodicals：Journal of experimental & clinical cancer research : CR

IF：11.300

Article：MicroRNA miR-1275 coordinately regulates AEA/LPA signals via targeting FAAH in lipid metabolism reprogramming of gastric cancer(miRNA)

Periodicals：Cell Death and Disease

IF：9.697

Article：Interference of a mammalian circRNA regulates lipid metabolism reprogramming by targeting miR-24-3p/Igf2/PI3K-AKT-mTOR and Igf2bp2/Ucp1 axis.

Periodicals：Cellular and molecular life sciences

IF：6.200

Article： CircZCCHC2 (hsa_circ_0000854) promotes hepatocellular carcinoma progression through modulating miR-936/BTBD7 axis and activating Rho/ROCK2 pathway. 

Periodicals：Non-coding RNA research

IF：5.900

Article：Marsdenia tenacissima extract prevents the malignant progression of glioma through upregulating lncRNA MEG3 and SFRP1-dependent inhibition of Wnt/β-catenin pathway

Periodicals：CNS Neuroscience & Therapeutics

IF：5.500

Article：Circular RNA hsa_circ_0005218 promotes the early recurrence of hepatocellular carcinoma by targeting the miR-31-5p/CDK1 pathway

Periodicals：Heliyon

IF：3.776

Article：SLERT, as a novel biomarker, orchestrates endometrial cancer metastasis via regulation of BDNF/TRKB signaling

Periodicals：World Journal of Surgical Oncology

IF：3.253

Article：Screening and Bioinformatics Analyses of Key miRNAs Associated with Toll-like Receptor Activation in Gastric Cancer Cells

Periodicals：Medicina

IF：2.948

【2022年】

Article：TLR4 regulates RORγt+ regulatory T-cell responses and susceptibility to colon infammation through interaction with Akkermansia muciniphila

Periodicals：Microbiome

IF：16.836

Article：Role of lncSLCO1C1 in gastric cancer progression and resistance to oxaliplatin therapy

Periodicals：Clinical and Translational Medicine

IF：11.492

Article：circCYP24A1 promotes Docetaxel resistance in prostate Cancer by Upregulating ALDH1A3

Periodicals：Biomarker Research

IF：8.633

Article：Serum-derived extracellular vesicles facilitate temozolomide resistance in glioblastoma through a HOTAIR-dependent mechanism

Periodicals：Cell Death & Disease

IF：8.469

Article：SIX5-activated LINC01468 promotes lung adenocarcinoma progression by recruiting SERBP1 to regulate SERPINE1 mRNA stability and recruiting USP5 to facilitate PAI1 protein deubiquitylation

Periodicals：Cell Death & Disease

IF：8.469

Article：Bone marrow mesenchymal stem cells inhibit hepatic fbrosis via the AABR07028795.2/rno-miR-667-5p axis

Periodicals：Stem Cell Research & Therapy

IF：8.08

Article：Circular RNA circLAMA3 inhibits the proliferation of bladder cancer by directly binding an mRNA

Periodicals：Molecular Therapy: Oncolytics

IF：7.2

Article：Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR-558 and regulating TREM1 expression

Periodicals：Cell Proliferation

IF：6.83

Article：OLMALINC/OCT4/BMP2 axis enhances osteogenic-like phenotype of renal interstitial fbroblasts to participate in Randall’s plaque formation

Periodicals：Molecular Medicine

IF：6.382

Article：circDNMT1 Promotes Malignant Progression of Gastric Cancer Through Targeting miR-576-3p/Hypoxia Inducible Factor-1 Alpha Axis

Periodicals：Frontiers in Oncology

IF：6.243

Article：Long noncoding RNA CASC7 is a novel regulator of glycolysis in oesophageal cancer via a miR-143-3p-mediated HK2 signalling pathway

Periodicals：Cell Death Discovery

IF：5.24

Article：Long non-coding RNA lncC11orf54-1 modulates neuroinfammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

Periodicals：Molecular Brain

IF：4.04

Article：MiR-122-5p promotes peritoneal fibrosis in a rat model of peritoneal dialysis by targeting Smad5 to activate Wnt/β-catenin pathway

Periodicals：Renal Failure

IF：2.6

【2021年】

Article：ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking

Periodicals：nature communications

IF：12.298

Article：Long noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1

Periodicals：Clinical and translational medicine

IF：11.493

Article：CircRNA Chordc1 protects mice from abdominal aortic aneurysm by contributing to the phenotype and growth of vascular smooth muscle cells

Periodicals：Molecular Therapy: Nucleic Acids

IF：8.886

Article：Oscillating lncRNA Platr4 regulates NLRP3 inflammasome to ameliorate nonalcoholic steatohepatitis in mice

Periodicals：Theranostics

IF：8.698

Article：circATP2B1 Promotes Aerobic Glycolysis in Gastric Cancer Cells Through Regulation of the miR-326 Gene Cluster

Periodicals：Frontiers in Oncology

IF：7.561

Article：Global identification of full‐length cassava lncRNAs unveils the role of cold‐responsive intergenic lncRNA 1 in cold stress response

Periodicals：Plant, Cell & Environment

IF：7.2

Article：Hsa_circ_0000652 Aggravates Inflammation by Activation of Macrophages and Enhancement of OX40/OX40L Interaction in Ankylosing Spondylitis

Periodicals：Frontiers in Cell and Developmental Biology

IF：6.68

Article：Identification of low-dose radiation-induced exosomal circMETRN and miR-4709-3p/GRB14/PDGFRα pathway as a key regulatory mechanism in Glioblastoma progression and radioresistance: Functional validation and clinical theranostic significance

Periodicals：International Journal of Biological Sciences

IF：6.582

Article：LncRNA Nqo1-AS1 Attenuates Cigarette Smoke-Induced Oxidative Stress by Upregulating its Natural Antisense Transcript Nqo1

Periodicals：Frontiers in Pharmacology

IF：5.811

Article：Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction

Periodicals：Aging

IF：5.682

Article：Paeonol inhibits the progression of intracerebral haemorrhage by mediating the HOTAIR/UPF1/ACSL4 axis

Periodicals：ASN Neuro

IF：4.146

Article：Tumor-derived exosomal circRNA051239 promotes proliferation and migration of epithelial ovarian cancer

Periodicals：Am J Transl Res

IF：3.377