荧光原位杂交(FISH)试剂盒
实验介绍

伯信生物建立了荧光原位杂交探针(FISH Probe)制备平台,承接荧光原位杂交试剂盒定制业务,可根据客户需求进行个性化定制。该试剂盒主要用于检测样本中目的基因的表达量与表达部位,可应用于组织切片(石蜡切片/冰冻切片)、细胞爬片与染色体片等的检测。 (本试剂盒仅供科研用途)

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详细介绍

伯信生物建立了荧光原位杂交探针(FISH Probe)制备平台,承接荧光原位杂交试剂盒定制业务,可根据客户需求进行个性化定制。


该试剂盒主要用于检测样本中目的基因的表达量与表达部位,可应用于组织切片(石蜡切片/冰冻切片)、细胞爬片与染色体片等的检测。


伯信生物采用从研究物种自体获取特异性探针并进行化学共价方式与荧光素连接。


自体荧光探针具有明显的优越性:灵敏度高,光稳定性好,荧光寿命长;最大的优势就是自体探针可以有效的降低自体的非特异荧光干扰,达到更加高效特异的基因检测信号。


通过对比将会产生极大的技术效应,获取客户的认可并替代传统的探针检测,满足国内外科研工作者对高质量的荧光探针原位杂交试剂盒的实验需求。


伯信生物荧光原位杂交试剂盒,自上市以来深受广大科研用户的欢迎和肯定,为中国科学院、中山大学医学院及附属医院、南方医科大学医学院及附属医院、香港大学、北京大学医学部及附属医院、东南大学医学院、江西农业大学、华中科技大学同济医学院等国内一流科研院所、医疗机构及跨国生物企业等近千家用户提供了优质的服务。


同时经过多年的积累,2011年建立了分子探针研发中心,伯信生物拥有强大的荧光原位杂交技术团队,为客户在试剂盒使用中遇到的各种问题给予技术支持和解答。




目前公司拥有库存现货试剂盒种类已达近万种:其中mRNA 1245种;microRNA 3171种,种属涵盖人、大小鼠、兔、猪、鸡、细菌、昆虫类等等,以及其他常规染色体探针及种属特异性探针等。


实验原理:


荧光原位杂交(Fluorescent in situ hybridization, FISH)是根据待测核酸序列设计特异性的荧光素标记寡聚核苷酸探针,再经过共变性-退火-复性,使探针与靶DNA/RNA按照碱基互补配对原则形成杂交体,最后利用荧光显微镜直接检测荧光信号,从而对组织、细胞中的待测核酸进行定性、半定量或相对定位分析的一种原位杂交技术。


技术流程:





结果实例:



                                                           mRNA FISH


                                                                lncRNA FISH



                                                              circRNA FISH


     

                                                          DNA FISH



产品优势


1. 探针制备采用直接标记法,省去了信号放大系统。

2. 荧光信号强,结果判定直观可靠。

3. 根据客户需求定制个性化探针。

4. 特异性强,灵敏度高,背景低。

5. 探针性能稳定,低温保存一年以上。

6. 同时示踪核酸和蛋白的定位情况。

7. 操作简单,安全、快速,重复性好。



详见产品说明书Bes1001 RNA-FISH(短链)

详见产品说明书Bes1002 RNA-FISH(长链) 

详见产品说明书Bes1011 DNA-FISH


文章案例


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ArticleCircular RNA 0001073 Attenuates Malignant Biological Behaviours in Breast Cancer Cell and Is Delivered by Nanoparticles to Inhibit Mice Tumour Growth

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ArticleKnockdown of circ-ABCB10 promotes sensitivity of lung cancer cells to cisplatin via miR-556-3p/AK4 axis

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ArticleCircRNA Cdr1as functions as a competitive endogenous RNA to promote hepatocellular carcinoma progression

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ArticleMicroRNA-1231 exerts a tumor suppressor role through regulating the EGFR/PI3K/AKT axis in glioma

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2017


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ArticleSilencing nc886, a Non-Coding RNA, Induces Apoptosis of Human Endometrial Cancer Cells-1A In Vitro

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2016


ArticlemiR-3188 regulates nasopharyngeal carcinoma proliferation and chemosensitivity through a FOXO1-modulated positive feedback loop with mTORp-PI3K/AKT-c-JUN

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ArticleLet-7f Regulates the Hypoxic Response in Cerebral Ischemia by Targeting NDRG3

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2013


ArticleAn EAV-HP Insertion in 59 Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken

PeriodicalsPLOS Genetics

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