伯信生物建立了荧光原位杂交探针（FISH Probe）制备平台，承接荧光原位杂交试剂盒定制业务，可根据客户需求进行个性化定制。
该试剂盒主要用于检测样本中目的基因的表达量与表达部位，可应用于组织切片（石蜡切片/冰冻切片）、细胞爬片与染色体片等的检测。
伯信生物采用从研究物种自体获取特异性探针并进行化学共价方式与荧光素连接。
自体荧光探针具有明显的优越性：灵敏度高，光稳定性好，荧光寿命长；最大的优势就是自体探针可以有效的降低自体的非特异荧光干扰，达到更加高效特异的基因检测信号。
通过对比将会产生极大的技术效应，获取客户的认可并替代传统的探针检测，满足国内外科研工作者对高质量的荧光探针原位杂交试剂盒的实验需求。
伯信生物荧光原位杂交试剂盒，自上市以来深受广大科研用户的欢迎和肯定，为中国科学院、中山大学医学院及附属医院、南方医科大学医学院及附属医院、香港大学、北京大学医学部及附属医院、东南大学医学院、江西农业大学、华中科技大学同济医学院等国内一流科研院所、医疗机构及跨国生物企业等近千家用户提供了优质的服务。
同时经过多年的积累，2011年建立了分子探针研发中心，伯信生物拥有强大的荧光原位杂交技术团队，为客户在试剂盒使用中遇到的各种问题给予技术支持和解答。
目前公司拥有库存现货试剂盒种类已达近万种：其中mRNA 1245种；microRNA 3171种，种属涵盖人、大小鼠、兔、猪、鸡、细菌、昆虫类等等，以及其他常规染色体探针及种属特异性探针等。
实验原理：
荧光原位杂交（Fluorescent in situ hybridization, FISH）是根据待测核酸序列设计特异性的荧光素标记寡聚核苷酸探针，再经过共变性-退火-复性，使探针与靶DNA/RNA按照碱基互补配对原则形成杂交体，最后利用荧光显微镜直接检测荧光信号，从而对组织、细胞中的待测核酸进行定性、半定量或相对定位分析的一种原位杂交技术。
技术流程：
结果实例：
mRNA FISH
lncRNA FISH
circRNA FISH
DNA FISH
产品优势：
1. 探针制备采用直接标记法，省去了信号放大系统。
2. 荧光信号强，结果判定直观可靠。
3. 根据客户需求定制个性化探针。
4. 特异性强，灵敏度高，背景低。
5. 探针性能稳定，低温保存一年以上。
6. 同时示踪核酸和蛋白的定位情况。
7. 操作简单，安全、快速，重复性好。
详见产品说明书：Bes1001 RNA-FISH（短链）
详见产品说明书：Bes1002 RNA-FISH（长链）
详见产品说明书：Bes1011 DNA-FISH
文章案例
【2023年】
Article：circEIF3I facilitates the recruitment of SMAD3 to early endosomes to promote TGF-β signalling pathway-mediated activation of MMPs in pancreatic cancer
Periodicals：Molecular Cancer
IF：37.299
Article：CircFOXK2 promotes hepatocellular carcinoma progression and leads to a poor clinical prognosis via regulating the Warburg efect
Periodicals：Journal of Experimental &Clinical Cancer Research
IF：12.658
Article：MicroRNA miR-1275 coordinately regulates AEA/LPA signals via targeting FAAH in lipid metabolism reprogramming of gastric cancer
Periodicals：Cell Death and Disease
IF：9.697
Article：Marsdenia tenacissima extract prevents the malignant progression of glioma through upregulating lncRNA MEG3 and SFRP1-dependent inhibition of Wnt/β-catenin pathway
Periodicals：CNS Neuroscience & Therapeutics
IF：5.500
Article：Circular RNA hsa_circ_0005218 promotes the early recurrence of hepatocellular carcinoma by targeting the miR-31-5p/CDK1 pathway
Periodicals：Heliyon
IF：3.776
Article：SLERT, as a novel biomarker, orchestrates endometrial cancer metastasis via regulation of BDNF/TRKB signaling
Periodicals：World Journal of Surgical Oncology
IF：3.253
Article：Screening and Bioinformatics Analyses of Key miRNAs Associated with Toll-like Receptor Activation in Gastric Cancer Cells
Periodicals：Medicina
IF：2.948
【2022年】
Article：TLR4 regulates RORγt+ regulatory T-cell responses and susceptibility to colon infammation through interaction with Akkermansia muciniphila
Periodicals：Microbiome
IF：16.836
Article：Role of lncSLCO1C1 in gastric cancer progression and resistance to oxaliplatin therapy
Periodicals：Clinical and Translational Medicine
IF：11.492
Article：circCYP24A1 promotes Docetaxel resistance in prostate Cancer by Upregulating ALDH1A3
Periodicals：Biomarker Research
IF：8.633
Article：Serum-derived extracellular vesicles facilitate temozolomide resistance in glioblastoma through a HOTAIR-dependent mechanism
Periodicals：Cell Death & Disease
IF：8.469
Article：SIX5-activated LINC01468 promotes lung adenocarcinoma progression by recruiting SERBP1 to regulate SERPINE1 mRNA stability and recruiting USP5 to facilitate PAI1 protein deubiquitylation
Periodicals：Cell Death & Disease
IF：8.469
Article：Bone marrow mesenchymal stem cells inhibit hepatic fbrosis via the AABR07028795.2/rno-miR-667-5p axis
Periodicals：Stem Cell Research & Therapy
IF：8.08
Article：Circular RNA circLAMA3 inhibits the proliferation of bladder cancer by directly binding an mRNA
Periodicals：Molecular Therapy: Oncolytics
IF：7.2
Article：Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR-558 and regulating TREM1 expression
Periodicals：Cell Proliferation
IF：6.83
Article：OLMALINC/OCT4/BMP2 axis enhances osteogenic-like phenotype of renal interstitial fbroblasts to participate in Randall’s plaque formation
Periodicals：Molecular Medicine
IF：6.382
Article：circDNMT1 Promotes Malignant Progression of Gastric Cancer Through Targeting miR-576-3p/Hypoxia Inducible Factor-1 Alpha Axis
Periodicals：Frontiers in Oncology
IF：6.243
Article：Long noncoding RNA CASC7 is a novel regulator of glycolysis in oesophageal cancer via a miR-143-3p-mediated HK2 signalling pathway
Periodicals：Cell Death Discovery
IF：5.24
Article：Long non-coding RNA lncC11orf54-1 modulates neuroinfammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection
Periodicals：Molecular Brain
IF：4.04
Article：MiR-122-5p promotes peritoneal fibrosis in a rat model of peritoneal dialysis by targeting Smad5 to activate Wnt/β-catenin pathway
Periodicals：Renal Failure
IF：2.6
【2021年】
Article：ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking
Periodicals：nature communications
IF：12.298
Article：Long noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1
Periodicals：Clinical and translational medicine
IF：11.493
Article：CircRNA Chordc1 protects mice from abdominal aortic aneurysm by contributing to the phenotype and growth of vascular smooth muscle cells
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.886
Article：Oscillating lncRNA Platr4 regulates NLRP3 inflammasome to ameliorate nonalcoholic steatohepatitis in mice
Periodicals：Theranostics
IF：8.698
Article：circATP2B1 Promotes Aerobic Glycolysis in Gastric Cancer Cells Through Regulation of the miR-326 Gene Cluster
Periodicals：Frontiers in Oncology
IF：7.561
Article：Global identification of full‐length cassava lncRNAs unveils the role of cold‐responsive intergenic lncRNA 1 in cold stress response
Periodicals：Plant, Cell & Environment
IF：7.2
Article：Hsa_circ_0000652 Aggravates Inflammation by Activation of Macrophages and Enhancement of OX40/OX40L Interaction in Ankylosing Spondylitis
Periodicals：Frontiers in Cell and Developmental Biology
IF：6.68
Article：Identification of low-dose radiation-induced exosomal circMETRN and miR-4709-3p/GRB14/PDGFRα pathway as a key regulatory mechanism in Glioblastoma progression and radioresistance: Functional validation and clinical theranostic significance
Periodicals：International Journal of Biological Sciences
IF：6.582
Article：LncRNA Nqo1-AS1 Attenuates Cigarette Smoke-Induced Oxidative Stress by Upregulating its Natural Antisense Transcript Nqo1
Periodicals：Frontiers in Pharmacology
IF：5.811
Article：Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction
Periodicals：Aging
IF：5.682
Article：Paeonol inhibits the progression of intracerebral haemorrhage by mediating the HOTAIR/UPF1/ACSL4 axis
Periodicals：ASN Neuro
IF：4.146
Article：Tumor-derived exosomal circRNA051239 promotes proliferation and migration of epithelial ovarian cancer
Periodicals：Am J Transl Res
IF：3.377
Article：Identification and Functional Prediction of Long Non-Coding RNAs in Dilated Cardiomyopathy by Bioinformatics Analysis
Periodicals：Frontiers in Genetics
IF：3.256
Article：Role of long intergenic non-protein coding RNA 01857 in hepatocellular carcinoma malignancy via the regulation of the microRNA-197-3p/anterior GRadient 2 axis
Periodicals：PLOS ONE
IF：3.24
Article：Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer
Periodicals：Oncology Letters
IF：2.967
Article：MicroRNA-488-3p Regulates Neuronal Cell Death in Cerebral Ischemic Stroke Through Vacuolar Protein Sorting 4B (VPS4B)
Periodicals：Neuropsychiatric Disease and Treatment
IF：2.57
Article：lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression
Periodicals：Experimental and Therapeutic Medicine
IF：2.447
Article：Long non‑coding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA‑588 in colorectal cancer
Periodicals：Oncology Letters
IF：2.311
【2020年】
Article：SRSF10 inhibits biogenesis of circ-ATXN1 to regulate glioma angiogenesis via miR-526b-3p/MMP2 pathway
Periodicals：Journal of Experimental & Clinical Cancer Research
IF：11.161
Article：MicroRNA-3613-5p Promotes Lung Adenocarcinoma Cell Proliferation through a RELA and AKT/MAPK Positive Feedback Loop
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.889
Article：circRNA Hipk3 Induces Cardiac Regeneration after Myocardial Infarction in Mice by Binding to Notch1 and miR-133a
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.889
Article：miR-210-5p promotes epithelial–mesenchymal transition by inhibiting PIK3R5 thereby activating oncogenic autophagy in osteosarcoma cells
Periodicals：Cell Death and Disease
IF：8.469
Article：Long non-coding RNA LRRC75A-AS1 facilitates triple negative breast cancer cell proliferation and invasion via functioning as a ceRNA to modulate
BAALC
Periodicals：Cell Death and Disease
IF：8.469
Article：LncSSBP1 Functions as a Negative Regulator of IL-6 Through Interaction With hnRNPK in Bronchial Epithelial Cells Infected With Talaromyces marneffei
Periodicals：Frontiers in Immunology
IF：7.561
Article：RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway
Periodicals：Molecular Therapy: Oncolytics
IF：7.2
Article：Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression
Periodicals：Journal of Experimental & Clinical Cancer Research
IF：7.038
Article：circ_2858 Helps Blood-Brain Barrier Disruption by Increasing VEGFA via Sponging miR-93-5p during Escherichia coli Meningitis
Periodicals： Molecular Therapy: Nucleic Acids
IF：7.032
Article：LncRNA FOXC2-AS1 enhances FOXC2 mRNA stability to promote colorectal cancer progression via activation of Ca2+-FAK signal pathway
Periodicals：Cell Death & Disease
IF：6.28
Article：SOX9-activated FARSA-AS1 predetermines cell growth, stemness, and metastasis in colorectal cancer through upregulating FARSA and SOX9
Periodicals：Cell Death & Disease
IF：6.28
Article：Circular RNA hsa_circ_0003141 promotes tumorigenesis of hepatocellular carcinoma via a miR-1827/UBAP2 axis
Periodicals：Aging
IF：5.682
Article：Long noncoding RNA TPT1-AS1 promotes the progression and metastasis of colorectal cancer by upregulating the TPT1-mediated FAK and JAK-STAT3 signalling pathways
Periodicals：Aging
IF：4.909
Article：Circular RNA circEXOC6B Inhibits the Progression of Ovarian Cancer by Sponging miR-421 and Regulating RUS1 Expression
Periodicals：OncoTargets and Therapy
IF：4.174
Article：LncRNA RP11‑86H7.1 promotes airway infammation induced byTRAPM2.5 by acting as a ceRNA of miRNA‑9‑5p to regulate NFKB1 in HBECS
Periodicals：Scientific Reports
IF：4.149
Article：LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer
Periodicals：Cancer Cell International
IF：3.991
Article：NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA
Periodicals：Cancer Cell International
IF：3.991
Article：Circ-PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR-542-3p/CDC5L/PEAK1 pathway
Periodicals：Cancer Medicine
IF：3.491
Article：Circular RNA 0001073 Attenuates Malignant Biological Behaviours in Breast Cancer Cell and Is Delivered by Nanoparticles to Inhibit Mice Tumour Growth
Periodicals：OncoTargets and Therapy
IF：3.259
Article：Knockdown of circ-ABCB10 promotes sensitivity of lung cancer cells to cisplatin via miR-556-3p/AK4 axis
Periodicals：BMC Pulmonary Medicine
IF：2.87
【2019年】
Article：Platelet-derived miR-223 promotes a phenotypic switch in arterial injury repair
Periodicals：The Journal of Clinical Investigation
IF：14.807
Article：Extracellular Vesicles from Albumin-Induced Tubular Epithelial Cells Promote the M1 Macrophage Phenotype by Targeting Klotho
Periodicals：Molecular Therapy
IF：11.455
Article：circAMOTL1 Motivates AMOTL1 Expression to Facilitate Cervical Cancer Growth
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.889
Article：Long noncoding RNA GAS5 induces abdominal aortic aneurysm formation by promoting smooth muscle apoptosis
Periodicals：Theranostics
IF：8.698
Article：Biosynthetic CircRNA_001160 induced by PTBP1 regulates the permeability of BTB via the CircRNA_001160/miR-195-5p/ETV1 axis
Periodicals：Cell Death and Disease
IF：8.469
Article：Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation
Periodicals：Therapeutic Advances in Medical Oncology
IF：8.168
Article：Exosomal transfer of miR-1238 contributes to temozolomide-resistance in glioblastoma
Periodicals：EBioMedicine
IF：8.142
Article：MicroRNA-206 attenuates glioma cell proliferation, migration, and invasion by blocking the WNT/β-catenin pathway via direct targeting of Frizzled 7 mRNA
Periodicals：American Journal of Translational Research
IF：6.166
Article：MicroRNA-940 inhibits glioma cells proliferation and cell cycle progression by targeting CKS1
Periodicals：American Journal of Translational Research
IF：6.166
Article：CircRNA Cdr1as functions as a competitive endogenous RNA to promote hepatocellular carcinoma progression
Periodicals：Aging
IF：5.682
Article：Hsa_circ_0001946 Inhibits Lung Cancer Progression and Mediates Cisplatin Sensitivity in Non-small Cell Lung Cancer via the Nucleotide Excision Repair Signaling Pathway
Periodicals：Frontiers in Oncology
IF：4.848
Article：Circular RNAs in human and vertebrate neural retinas
Periodicals：RNA Biology
IF：4.822
Article：Long non-coding RNA F11-AS1 inhibits HBV-related hepatocellular carcinoma progression by regulating NR1I3 via binding to microRNA-211-5p
Periodicals：Journal of Cellular and Molecular Medicine
IF：4.438
Article：Circular HDAC9/microRNA-138/Sirtuin-1 Pathway Mediates Synaptic and Amyloid Precursor Protein Processing Deficits in Alzheimer’s Disease
Periodicals：Neuroscience Bulletin
IF：4.326
Article：Mechanism of aberrant long non-coding RNA expression in an adriamycin-resistant liver cancer cell strain
Periodicals：Digestive and Liver Disease
IF：4.088
Article：Long noncoding RNA SNHG6 functions as a competing endogenous RNA by sponging miR-181a-5p to regulate E2F5 expression in colorectal cancer
Periodicals：Cancer Management and Research
IF：3.989
Article：Circular HDAC9/microRNA-138/Sirtuin-1 Pathway Mediates Synaptic and Amyloid Precursor Protein Processing Deficits in Alzheimer’s Disease
Periodicals：Neuroscience Bulletin
IF：3.903
Article：High-throughput sequencing reveals circular RNA hsa circ 0000592 as a novel player in the carcinogenesis of gastric carcinoma
Periodicals：Bioscience Reports
IF：3.411
【2018年】
Article：Multistage Delivery Nanoparticle Facilitates Efficient CRISPR/dCas9 Activation and Tumor Growth Suppression In Vivo
Periodicals：Advanced Science
IF：15.804
Article：circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.889
Article：FEZF1-AS1/miR-107/ZNF312B axis facilitates progression and Warburg effect in pancreatic ductal adenocarcinoma
Periodicals：Cell Death and Disease
IF：8.469
Article：TSG-6 attenuates inflammation-induced brain injury via modulation of microglial polarization in SAH rats through the SOCS3/STAT3 pathway
Periodicals：Journal of Neuroinflammation
IF：5.791
Article：MiR-422a regulates cellular metabolism and malignancy by targeting pyruvate dehydrogenase kinase 2 in gastric cancer
Periodicals：Cell Death & Disease
IF：5.638
Article：TSG-6 attenuates inflammation-induced brain injury via modulation of microglial polarization in SAH rats through the SOCS3/STAT3 pathway
Periodicals：Journal of Neuroinflammation
IF：5.193
Article：Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 promotes lung adenocarcinoma by directly interacting with specificity protein 1
Periodicals：Cancer Science
IF：4.967
Article：MicroRNA-1231 exerts a tumor suppressor role through regulating the EGFR/PI3K/AKT axis in glioma
Periodicals：Journal of Neuro-Oncology
IF：4.129
【2017年】
Article：LncRNA-SARCC suppresses renal cell carcinoma(RCC) progression via altering the androgen receptor(AR)/miRNA-143-3p signals
Periodicals：Cell Death and Differentiation
IF：15.828
Article：Up-regulation of lncRNA CASC9 promotes esophageal squamous cell carcinoma growth by negatively regulating PDCD4 expression through EZH2
Periodicals：Molecular Cancer
IF：15.302
Article：Inhibition of TDP43-Mediated SNHG12-miR-195-SOX5 Feedback Loop Impeded Malignant Biological Behaviors of Glioma Cells
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.889
Article：miR-1301 inhibits hepatocellular carcinoma cell migration, invasion, and angiogenesis by decreasing Wnt/β-catenin signaling through targeting BCL9
Periodicals：Cell Death and Disease
IF：8.469
Article：Up-regulation of lncRNA CASC9 promotes esophageal squamous cell carcinoma growth by negatively regulating PDCD4 expression through EZH2
Periodicals：Molecular Cancer
IF：7.776
Article：TTBK2 circular RNA promotes glioma malignancy by regulating miR-217/HNF1β/Derlin-1 pathway
Periodicals：Journal of Hematology & Oncology
IF：7.333
Article：p53 dynamics orchestrates with binding affinity to target genes for cell fate decision
Periodicals：Cell Death & Disease
IF：6.304
Article：MicroRNA-936 induces cell cycle arrest and inhibits glioma cell proliferation by targeting CKS1
Periodicals：Am J Cancer Res
IF：6.166
Article：MicroRNA-1301 inhibits proliferation of human glioma cells by directly targeting N-Ras
Periodicals：Am J Cancer Res
IF：6.166
Article：MicroRNA-520d-5p inhibits human glioma cell proliferation and induces cell cycle arrest by directly targeting PTTG1
Periodicals：Am J Cancer Res
IF：6.166
Article：MicroRNA-1468-5p inhibits glioma cell proliferation and induces cell cycle arrest by targeting RRM1
Periodicals：Am J Cancer Res
IF：6.166
Article：MicroRNA-625 inhibits the proliferation and increases the chemosensitivity of glioma by directly targeting AKT2
Periodicals：Am J Cancer Res
IF：6.166
Article：p53 dynamics orchestrates with binding affinity to target genes for cell fate decision
Periodicals：Cell Death & Disease
IF：5.638
Article：lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis
Periodicals：Toxicological Sciences
IF：4.181
Article：Long noncoding RNA SFTA1P promoted apoptosis and increased cisplatin chemosensitivity via regulating the hnRNP-U-GADD45A axis in lung squamous cell carcinoma
Periodicals：Oncotarget
IF：3.765
Article：OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation
Periodicals：BioMed Research International
IF：3.411
Article：Systematic identification and characterization of long non-coding RNAs in mouse mature sperm
Periodicals：PLOS ONE
IF：2.766
Article：Calcitonin gene‑related peptide induces IL‑6 expression in RAW264.7 macrophages mediated by mmu_circRNA_007893
Periodicals：Molecular Medicine Reports
IF：2.085
Article：Calcitonin gene‑related peptide induces IL‑6 expression in RAW264.7 macrophages mediated by mmu_circRNA_007893
Periodicals：Molecular Medicine Reports
IF：1.922
Article：Silencing nc886, a Non-Coding RNA, Induces Apoptosis of Human Endometrial Cancer Cells-1A In Vitro
Periodicals：Medical Science Monitor
IF：1.894
【2016年】
Article：miR-3188 regulates nasopharyngeal carcinoma proliferation and chemosensitivity through a FOXO1-modulated positive feedback loop with mTOR–p-PI3K/AKT-c-JUN
Periodicals：NATURE COMMUNICATIONS
IF：14.92
Article：A novel regulatory network among LncRpa, CircRar1, MiR-671 and apoptotic genes promotes lead-induced neuronal cell apoptosis
Periodicals：Archives of Toxicology
IF：5.728
Article：Infection of Common Marmosets with GB Virus B Chimeric Virus Encoding the Major Nonstructural Proteins NS2 to NS4A of Hepatitis C Virus
Periodicals：Journal of Virology
IF：5.103
Article：Let-7f Regulates the Hypoxic Response in Cerebral Ischemia by Targeting NDRG3
Periodicals：Neurochemical Research
IF：2.772
【2013年】
Article：An EAV-HP Insertion in 59 Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken
Periodicals：PLOS Genetics
IF：5.540
