
伯信生物建立了荧光原位杂交探针(FISH Probe)制备平台,承接荧光原位杂交试剂盒定制业务,可根据客户需求进行个性化定制。该试剂盒主要用于检测样本中目的基因的表达量与表达部位,可应用于组织切片(石蜡切片/冰冻切片)、细胞爬片与染色体片等的检测。 (本试剂盒仅供科研用途)
伯信生物建立了荧光原位杂交探针(FISH Probe)制备平台,承接荧光原位杂交试剂盒定制业务,可根据客户需求进行个性化定制。
该试剂盒主要用于检测样本中目的基因的表达量与表达部位,可应用于组织切片(石蜡切片/冰冻切片)、细胞爬片与染色体片等的检测。
伯信生物采用从研究物种自体获取特异性探针并进行化学共价方式与荧光素连接。
自体荧光探针具有明显的优越性:灵敏度高,光稳定性好,荧光寿命长;最大的优势就是自体探针可以有效的降低自体的非特异荧光干扰,达到更加高效特异的基因检测信号。
通过对比将会产生极大的技术效应,获取客户的认可并替代传统的探针检测,满足国内外科研工作者对高质量的荧光探针原位杂交试剂盒的实验需求。
伯信生物荧光原位杂交试剂盒,自上市以来深受广大科研用户的欢迎和肯定,为中国科学院、中山大学医学院及附属医院、南方医科大学医学院及附属医院、香港大学、北京大学医学部及附属医院、东南大学医学院、江西农业大学、华中科技大学同济医学院等国内一流科研院所、医疗机构及跨国生物企业等近千家用户提供了优质的服务。
同时经过多年的积累,2011年建立了分子探针研发中心,伯信生物拥有强大的荧光原位杂交技术团队,为客户在试剂盒使用中遇到的各种问题给予技术支持和解答。
目前公司拥有库存现货试剂盒种类已达近万种:其中mRNA 1245种;microRNA 3171种,种属涵盖人、大小鼠、兔、猪、鸡、细菌、昆虫类等等,以及其他常规染色体探针及种属特异性探针等。
实验原理:
荧光原位杂交(Fluorescent in situ hybridization, FISH)是根据待测核酸序列设计特异性的荧光素标记寡聚核苷酸探针,再经过共变性-退火-复性,使探针与靶DNA/RNA按照碱基互补配对原则形成杂交体,最后利用荧光显微镜直接检测荧光信号,从而对组织、细胞中的待测核酸进行定性、半定量或相对定位分析的一种原位杂交技术。
技术流程:

结果实例:
mRNA FISH
lncRNA FISH
circRNA FISH
DNA FISH
产品优势:
1. 探针制备采用直接标记法,省去了信号放大系统。
2. 荧光信号强,结果判定直观可靠。
3. 根据客户需求定制个性化探针。
4. 特异性强,灵敏度高,背景低。
5. 探针性能稳定,低温保存一年以上。
6. 同时示踪核酸和蛋白的定位情况。
7. 操作简单,安全、快速,重复性好。
文章案例
【2025年】
Periodicals:Clinical Epigenetics
IF:4.800
【2024年】
Article:LncRNA-Mediated TPI1 and PKM2 Promote Self-Renewal and Chemoresistance in GBM.
Periodicals:Advanced science (Weinheim, Baden-Wurttemberg, Germany)
IF:14.300
Periodicals:Environment International
IF:10.300
Article:Periodic changes of cyclin D1 mRNA stability are regulated by PC4 modifications in the cell cycle.
Periodicals:The Journal of Cell Biology
IF:7.400
Periodicals:iScience
IF:7.100
Periodicals:Molecular Therapy. Oncology
IF:5.299
Periodicals:Annals of Medicine
IF:4.900
Periodicals:International Journal of Molecular Sciences
IF:4.900
Periodicals:iScience
IF:4.600
Article:The IGF2BP2-lncRNA TRPC7-AS1 axis promotes hepatocellular carcinoma cell proliferation and invasion.
Periodicals:Cellular Signalling
IF:4.400
Periodicals:Journal of cell communication and signaling
IF:4.100
Article:Long non‑coding RNA DANCR aggravates breast cancer through the miR‑34c/E2F1 feedback loop.
Periodicals:Molecular medicine reports
IF:3.400
Article:lncRNA H19 facilitates vascular neointima formation by targeting miR-125a-3p/FLT1 axis.
Periodicals:Acta biochimica et biophysica Sinica
IF:3.300
Article:LncIRF1 promotes chicken resistance to ALV-J infection.
Periodicals:3 Biotech
IF:2.600
【2023年】
Periodicals:Molecular Cancer
IF:37.299
Article:Augmenting L3MBTL2-induced condensates suppresses tumor growth in osteosarcoma.
Periodicals:Science Advances
IF:13.600
Periodicals:Journal of Experimental &Clinical Cancer Research
IF:12.658
Periodicals:Translational Neurodegeneration
IF:12.600
Periodicals:Journal of experimental & clinical cancer research : CR
IF:11.300
Periodicals:Cell Death and Disease
IF:9.697
Periodicals:Cellular and molecular life sciences
IF:6.200
Periodicals:Non-coding RNA research
IF:5.900
Periodicals:CNS Neuroscience & Therapeutics
IF:5.500
Periodicals:Heliyon
IF:3.776
Periodicals:World Journal of Surgical Oncology
IF:3.253
Periodicals:Medicina
IF:2.948
【2022年】
Periodicals:Microbiome
IF:16.836
Article:Role of lncSLCO1C1 in gastric cancer progression and resistance to oxaliplatin therapy
Periodicals:Clinical and Translational Medicine
IF:11.492
Article:circCYP24A1 promotes Docetaxel resistance in prostate Cancer by Upregulating ALDH1A3
Periodicals:Biomarker Research
IF:8.633
Periodicals:Cell Death & Disease
IF:8.469
Periodicals:Cell Death & Disease
IF:8.469
Periodicals:Stem Cell Research & Therapy
IF:8.08
Article:Circular RNA circLAMA3 inhibits the proliferation of bladder cancer by directly binding an mRNA
Periodicals:Molecular Therapy: Oncolytics
IF:7.2
Periodicals:Cell Proliferation
IF:6.83
Periodicals:Molecular Medicine
IF:6.382
Periodicals:Frontiers in Oncology
IF:6.243
Periodicals:Cell Death Discovery
IF:5.24
Periodicals:Molecular Brain
IF:4.04
Periodicals:Renal Failure
IF:2.6
【2021年】
Article:ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking
Periodicals:nature communications
IF:12.298
Periodicals:Clinical and translational medicine
IF:11.493
Periodicals:Molecular Therapy: Nucleic Acids
IF:8.886
Periodicals:Theranostics
IF:8.698
Periodicals:Frontiers in Oncology
IF:7.561
Periodicals:Plant, Cell & Environment
IF:7.2
Periodicals:Frontiers in Cell and Developmental Biology
IF:6.68
Periodicals:International Journal of Biological Sciences
IF:6.582
Periodicals:Frontiers in Pharmacology
IF:5.811
Article:Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction
Periodicals:Aging
IF:5.682
Periodicals:ASN Neuro
IF:4.146
Periodicals:Am J Transl Res
IF:3.377
Periodicals:Frontiers in Genetics
IF:3.256
Periodicals:PLOS ONE
IF:3.24
Periodicals:Oncology Letters
IF:2.967
Periodicals:Neuropsychiatric Disease and Treatment
IF:2.57
Periodicals:Experimental and Therapeutic Medicine
IF:2.447
Periodicals:Oncology Letters
IF:2.311
【2020年】
Periodicals:Journal of Experimental & Clinical Cancer Research
IF:11.161
Periodicals:Molecular Therapy: Nucleic Acids
IF:8.889
Periodicals:Molecular Therapy: Nucleic Acids
IF:8.889
Periodicals:Cell Death and Disease
IF:8.469
Periodicals:Cell Death and Disease
IF:8.469
Periodicals:Frontiers in Immunology
IF:7.561
Article:RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway
Periodicals:Molecular Therapy: Oncolytics
IF:7.2
Periodicals:Journal of Experimental & Clinical Cancer Research
IF:7.038
Periodicals: Molecular Therapy: Nucleic Acids
IF:7.032
Periodicals:Cell Death & Disease
IF:6.28
Periodicals:Cell Death & Disease
IF:6.28
Periodicals:Aging
IF:5.682
Periodicals:Aging
IF:4.909
Periodicals:OncoTargets and Therapy
IF:4.174
Periodicals:Scientific Reports
IF:4.149
Periodicals:Cancer Cell International
IF:3.991
Periodicals:Cancer Cell International
IF:3.991
Periodicals:Cancer Medicine
IF:3.491
Periodicals:OncoTargets and Therapy
IF:3.259
Periodicals:BMC Pulmonary Medicine
IF:2.87
【2019年】
Article:Platelet-derived miR-223 promotes a phenotypic switch in arterial injury repair
Periodicals:The Journal of Clinical Investigation
IF:14.807
Periodicals:Molecular Therapy
IF:11.455
Article:circAMOTL1 Motivates AMOTL1 Expression to Facilitate Cervical Cancer Growth
Periodicals:Molecular Therapy: Nucleic Acids
IF:8.889
Periodicals:Theranostics
IF:8.698
Periodicals:Cell Death and Disease
IF:8.469
Periodicals:Therapeutic Advances in Medical Oncology
IF:8.168
Article:Exosomal transfer of miR-1238 contributes to temozolomide-resistance in glioblastoma
Periodicals:EBioMedicine
IF:8.142
Periodicals:American Journal of Translational Research
IF:6.166
Article:MicroRNA-940 inhibits glioma cells proliferation and cell cycle progression by targeting CKS1
Periodicals:American Journal of Translational Research
IF:6.166
Periodicals:Aging
IF:5.682
Periodicals:Frontiers in Oncology
IF:4.848
Article:Circular RNAs in human and vertebrate neural retinas
Periodicals:RNA Biology
IF:4.822
Periodicals:Journal of Cellular and Molecular Medicine
IF:4.438
Periodicals:Neuroscience Bulletin
IF:4.326
Periodicals:Digestive and Liver Disease
IF:4.088
Periodicals:Cancer Management and Research
IF:3.989
Periodicals:Neuroscience Bulletin
IF:3.903
Periodicals:Bioscience Reports
IF:3.411
【2018年】
Periodicals:Advanced Science
IF:15.804
Periodicals:Molecular Therapy: Nucleic Acids
IF:8.889
Periodicals:Cell Death and Disease
IF:8.469
Periodicals:Journal of Neuroinflammation
IF:5.791
Periodicals:Cell Death & Disease
IF:5.638
Periodicals:Journal of Neuroinflammation
IF:5.193
Periodicals:Cancer Science
IF:4.967
Article:MicroRNA-1231 exerts a tumor suppressor role through regulating the EGFR/PI3K/AKT axis in glioma
Periodicals:Journal of Neuro-Oncology
IF:4.129
【2017年】
Periodicals:Cell Death and Differentiation
IF:15.828
Periodicals:Molecular Cancer
IF:15.302
Periodicals:Molecular Therapy: Nucleic Acids
IF:8.889
Periodicals:Cell Death and Disease
IF:8.469
Periodicals:Molecular Cancer
IF:7.776
Article:TTBK2 circular RNA promotes glioma malignancy by regulating miR-217/HNF1β/Derlin-1 pathway
Periodicals:Journal of Hematology & Oncology
IF:7.333
Article:p53 dynamics orchestrates with binding affinity to target genes for cell fate decision
Periodicals:Cell Death & Disease
IF:6.304
Article:MicroRNA-936 induces cell cycle arrest and inhibits glioma cell proliferation by targeting CKS1
Periodicals:Am J Cancer Res
IF:6.166
Article:MicroRNA-1301 inhibits proliferation of human glioma cells by directly targeting N-Ras
Periodicals:Am J Cancer Res
IF:6.166
Periodicals:Am J Cancer Res
IF:6.166
Article:MicroRNA-1468-5p inhibits glioma cell proliferation and induces cell cycle arrest by targeting RRM1
Periodicals:Am J Cancer Res
IF:6.166
Periodicals:Am J Cancer Res
IF:6.166
Article:p53 dynamics orchestrates with binding affinity to target genes for cell fate decision
Periodicals:Cell Death & Disease
IF:5.638
Article:lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis
Periodicals:Toxicological Sciences
IF:4.181
Periodicals:Oncotarget
IF:3.765
Article:OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation
Periodicals:BioMed Research International
IF:3.411
Article:Systematic identification and characterization of long non-coding RNAs in mouse mature sperm
Periodicals:PLOS ONE
IF:2.766
Periodicals:Molecular Medicine Reports
IF:2.085
Periodicals:Molecular Medicine Reports
IF:1.922
Article:Silencing nc886, a Non-Coding RNA, Induces Apoptosis of Human Endometrial Cancer Cells-1A In Vitro
Periodicals:Medical Science Monitor
IF:1.894
【2016年】
Periodicals:NATURE COMMUNICATIONS
IF:14.92
Periodicals:Archives of Toxicology
IF:5.728
Periodicals:Journal of Virology
IF:5.103
Article:Let-7f Regulates the Hypoxic Response in Cerebral Ischemia by Targeting NDRG3
Periodicals:Neurochemical Research
IF:2.772
【2013年】
Article:An EAV-HP Insertion in 59 Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken
Periodicals:PLOS Genetics
IF:5.540