miRNA pulldown 试剂盒
实验介绍

miRNA pulldown是一项用于研究miRNA靶基因的技术。(本试剂盒仅供科研用途)

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详细介绍


实验原理:


将生物素标记的miRNA探针转染到细胞中,使细胞裂解物中的生物素标记miRNA-RNA复合物能够被链霉亲和素磁珠高效富集,从而与裂解液中其他成分分离;随后,通过洗脱纯化得到RNA并进行高通量测序或qPCR检测,验证miRNA的靶基因。此外,还可以通过洗脱纯化获得结合在RNA上的蛋白并进行质谱检测或Western blot检测。

 

产品优势:


1. 高品质,稳定性好。

2. 易用:操作简单,容易上手。

3. 精准:特异性高,背景少。


技术流程:


结果实例:




详见产品说明书:Bes5108 miRNA pulldown-RNA

详见产品说明书:Bes5109 miRNA pulldown-Protein



文章案例


2024


ArticleA tumor suppressor protein encoded by circKEAP1 inhibits osteosarcoma cell stemness and metastasis by promoting vimentin proteasome degradation and activating anti-tumor immunity.

Periodicals:Journal of experimental & clinical cancer research

IF:11.400


ArticleCircZCCHC2 decreases pirarubicin sensitivity and promotes triple-negative breast cancer development via the miR-1200/TPR axis.

Periodicals:iScience

IF5.800


Article Long noncoding RNA SNHG1 promotes breast cancer progression by regulating the miR-641/RRS1 axis.

Periodicals:Scientific reports

IF:4.600


ArticleCirc_0070934 promotes MGAT3 expression and inhibits epithelial-mesenchymal transition in bronchial epithelial cells by sponging miR-199a-5p. 

Periodicals:Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology

IF:2.600


2023


ArticleA novel circular RNA, circMAML3, promotes tumor progression of prostate cancer by regulating miR-665/MAPK8IP2 axis.

Periodicals:Cell Death Discovery

IF7.000


ArticleCircPLXNA2 Affects the Proliferation and Apoptosis of Myoblast through circPLXNA2/gga-miR-12207-5P/MDM4 Axis

PeriodicalsInternational Journal of Molecular Sciences

IF6.208


ArticleA novel circular RNA, circMAML3, promotes tumor progression of prostate cancer by regulating miR-665/MAPK8IP2 axis.

Periodicals:Cell death discovery

IF6.100


ArticleIntegrated analysis identifies microRNA-188-5p as a suppressor of AKT/mTOR pathway in renal cancer

Periodicals:Cancer Science

IF5.699


ArticleLncRNA ANRIL promotes HR repair through regulating PARP1 expression by sponging miR-7-5p in lung cancer

PeriodicalsBMC Cancer

IF4.638


2022


ArticleLong noncoding RNA TINCR facilitates hepatocellular carcinoma progression and dampens chemosensitivity to oxaliplatin by regulating the miR-195-3p/ST6GAL1/NF-κB pathway

PeriodicalsJournal of Experimental & Clinical Cancer Research

IF11.16


ArticleMiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4

PeriodicalsCell

IF7.666


ArticleCircDCLRE1C Regulated Lipopolysaccharide-Induced Inflammatory Response and Apoptosis by Regulating miR-214b-3p/STAT3 Pathway in Macrophages

PeriodicalsInternational Journal of Molecular Sciences

IF6.208


2021


ArticleCircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression

PeriodicalsGenes

IF4.096

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