实验原理：

将生物素标记的miRNA探针转染到细胞中，使细胞裂解物中的生物素标记miRNA-RNA复合物能够被链霉亲和素磁珠高效富集，从而与裂解液中其他成分分离；随后，通过洗脱纯化得到RNA并进行高通量测序或qPCR检测，验证miRNA的靶基因。此外，还可以通过洗脱纯化获得结合在RNA上的蛋白并进行质谱检测或Western blot检测。

 

产品优势：

1. 高品质，稳定性好。

2. 易用：操作简单，容易上手。

3. 精准：特异性高，背景少。

技术流程：

结果实例：

详见产品说明书：Bes5108 miRNA pulldown-RNA

详见产品说明书：Bes5109 miRNA pulldown-Protein

文章案例

【2023年】

Article：A novel circular RNA, circMAML3, promotes tumor progression of prostate cancer by regulating miR-665/MAPK8IP2 axis.

Periodicals：Cell Death Discovery

IF：7.000

Article：CircPLXNA2 Affects the Proliferation and Apoptosis of Myoblast through circPLXNA2/gga-miR-12207-5P/MDM4 Axis

Periodicals：International Journal of Molecular Sciences

IF：6.208

Article：Integrated analysis identifies microRNA-188-5p as a suppressor of AKT/mTOR pathway in renal cancer

Periodicals：Cancer Science

IF：5.699

Article：LncRNA ANRIL promotes HR repair through regulating PARP1 expression by sponging miR-7-5p in lung cancer

Periodicals：BMC Cancer

IF：4.638

【2022年】

Article：Long noncoding RNA TINCR facilitates hepatocellular carcinoma progression and dampens chemosensitivity to oxaliplatin by regulating the miR-195-3p/ST6GAL1/NF-κB pathway

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.16

Article：MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4

Periodicals：Cell

IF：7.666

Article：CircDCLRE1C Regulated Lipopolysaccharide-Induced Inflammatory Response and Apoptosis by Regulating miR-214b-3p/STAT3 Pathway in Macrophages

Periodicals：International Journal of Molecular Sciences

IF：6.208

【2021年】

Article：CircNFIC Balances Inflammation and Apoptosis by Sponging miR-30e-3p and Regulating DENND1B Expression

Periodicals：Genes

IF：4.096