地高辛(DIG)原位杂交试剂盒
实验介绍

伯信生物建立了地高辛原位杂交探针(ISH Probe)制备平台,承接定制地高辛原位杂交试剂盒业务,可根据客户需要进行个性化定制。该试剂盒主要用于检测样本中目的基因的表达量及表达位置,可用于组织切片(石蜡切片/冰冻切片)、细胞爬片、染色体片等的检测。 (本试剂盒仅供科研用途)

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详细介绍

伯信生物建立了地高辛原位杂交探针(ISH Probe)制备平台,承接定制地高辛原位杂交试剂盒业务,可根据客户需要进行个性化定制。该试剂盒主要用于检测样本中目的基因的表达量及表达位置,可用于组织切片(石蜡切片/冰冻切片)、细胞爬片及染色体片等的检测。


伯信生物地高辛原位杂交试剂盒,自上市以来深受广大科研用户的欢迎和肯定,为中国科学院、中山大学医学院及附属医院、南方医科大学医学院及附属医院、香港大学、北京大学医学部及附属医院、东南大学医学院、江西农业大学、华中科技大学同济医学院等国内一流科研院所、医疗机构及跨国生物企业等近千家用户提供了优质的服务。同时经过多年的积累,伯信生物拥有强大的原位杂交技术团队,为客户在试剂盒使用中遇到的各种问题给予技术支持和解答。


目前公司拥有库存现货试剂盒种类已达近千种:其中mRNA 约1100种;microRNA 约 500种;种属涵盖人、大小鼠、兔、猪、鸡、细菌、昆虫类等等。



实验原理:


显色原位杂交技术(Chromogenic in situ hybridization, CISH)是根据待测核酸序列设计特异性的末端标记寡聚核苷酸探针,再经过共变性-退火-复性,使探针与靶DNA/RNA按照碱基互补配对原则形成杂交体,最后利用光学显微镜检测杂交信号,从而对组织、细胞中的待测核酸进行定性、半定量或相对定位分析的一种原位杂交技术。

 


技术流程:









结果实例:





产品优势:


1. 信号清晰,定位准确,结果判定直观可靠。

2. 根据客户需求定制个性化探针。

3. 特异性强,灵敏度高,背景低。

4. 探针性能稳定,低温保存一年以上。

5. 操作简单,安全、快速,重复性好。




详见产品说明书Bes1006 CISH(短链)

详见产品说明书Bes1007 CISH(长链)



文章案例



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