伯信生物建立了地高辛原位杂交探针（ISH Probe）制备平台，承接定制地高辛原位杂交试剂盒业务，可根据客户需要进行个性化定制。该试剂盒主要用于检测样本中目的基因的表达量及表达位置，可用于组织切片（石蜡切片/冰冻切片）、细胞爬片及染色体片等的检测。

伯信生物地高辛原位杂交试剂盒，自上市以来深受广大科研用户的欢迎和肯定，为中国科学院、中山大学医学院及附属医院、南方医科大学医学院及附属医院、香港大学、北京大学医学部及附属医院、东南大学医学院、江西农业大学、华中科技大学同济医学院等国内一流科研院所、医疗机构及跨国生物企业等近千家用户提供了优质的服务。同时经过多年的积累，伯信生物拥有强大的原位杂交技术团队，为客户在试剂盒使用中遇到的各种问题给予技术支持和解答。

目前公司拥有库存现货试剂盒种类已达近千种：其中mRNA 约1100种；microRNA 约 500种；种属涵盖人、大小鼠、兔、猪、鸡、细菌、昆虫类等等。

实验原理：

显色原位杂交技术（Chromogenic in situ hybridization, CISH）是根据待测核酸序列设计特异性的末端标记寡聚核苷酸探针，再经过共变性-退火-复性，使探针与靶DNA/RNA按照碱基互补配对原则形成杂交体，最后利用光学显微镜检测杂交信号，从而对组织、细胞中的待测核酸进行定性、半定量或相对定位分析的一种原位杂交技术。

 

技术流程：

结果实例：

产品优势：

1. 信号清晰，定位准确，结果判定直观可靠。

2. 根据客户需求定制个性化探针。

3. 特异性强，灵敏度高，背景低。

4. 探针性能稳定，低温保存一年以上。

5. 操作简单，安全、快速，重复性好。

详见产品说明书：Bes1006 CISH（短链）

详见产品说明书：Bes1007 CISH（长链）

文章案例

【2025年】

Article：Effect of miR-6767-5p on breast cancer cell phenotype and its regulatory mechanism. 

Periodicals：Scientific Reports

IF：3.900

Article：Cancer cell-derived exosomal miR-34a inhibits the malignant progression of pancreatic adenocarcinoma cells by restraining the M2 polarization of macrophages.

Periodicals：European Journal of Histochemistry: EJH

IF：2.100

【2023年】

Article：Downregulation of Linc00173 increases BCL2 mRNA stability via the miR-1275/PROCA1/ZFP36L2 axis and induces acquired cisplatin resistance of lung adenocarcinoma

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.300

Article：Long non-coding RNA FENDRR suppresses cancer-associated fibroblasts and serves as a prognostic indicator in colorectal cancer

Periodicals：Translational Oncology

IF：5.002

Article：Role of LncRNA MIR99AHG in breast cancer: Bioinformatic analysis and preliminary verification

Periodicals：Heliyon

IF：4.000

【2022年】

Article：Fusobacterium nucleatum induces excess methyltransferaselike 3-mediated microRNA-4717-3p maturation to promote colorectal cancer cell proliferation

Periodicals：Cancer Science

IF：5.700

Article：Effects of miR-939 and miR-376A on ulcerative colitis using a decoy strategy to inhibit NF-κB and NFAT expression

Periodicals：European Journal of Histochemistry

IF：2.000

【2020年】

Article：An NF-κB–driven lncRNA orchestrates colitis and circadian clock

Periodicals：SCIENCE ADVANCES

IF：13.600

Article：MiR-4310 induced by SP1 targets PTEN to promote glioma progression

Periodicals：Cancer Cell International

IF：5.800

Article：lncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR‑106a‑5p‑mediated P38 MAPK signaling

Periodicals：Molecular Medicine Reports

IF：3.400

【2019年】

Article：Lnc-TALC promotes O6-methylguanine-DNA methyltransferase expression via regulating the c-Met pathway by competitively binding with miR-20b-3p

Periodicals：NATURE COMMUNICATIONS

IF：12.122

Article：HBX-induced miR-5188 impairs FOXO1 to stimulate β-catenin nuclear translocation and promotes tumor stemness in hepatocellular carcinoma

Periodicals：Theranostics

IF：8.698

Article：Timeless-Stimulated miR-5188-FOXO1/b-Cateninc-Jun Feedback Loop Promotes Stemness via Ubiquitination of b-Catenin in Breast Cancer

Periodicals：Molecular Therapy

IF：7.97

Article：The long noncoding RNA AK002107 negatively modulates miR-140-5p and targets TGFBR1 to induce epithelial-mesenchymal transition in hepatocellular carcinoma

Periodicals：Molecular Oncology

IF：6.570

Article：Cancer-associated fibroblasts promote PD-L1 expression in mice cancer cells via secreting CXCL5

Periodicals：International Journal of Cancer

IF：6.4

【2018年】

Article：RETRACTED ARTICLE: Dual roles of miR-374a by modulated c-Jun respectively targets CCND1-inducing PI3K/AKT signal and PTEN-suppressing Wnt/β-catenin signaling in non-small-cell lung cancer

Periodicals：Cell Death & Disease

IF：5.638

Article：Inhibition of microRNA-16 facilitates the paclitaxel resistance by targeting IKBKB via NF-kB signaling pathway in hepatocellular carcinoma

Periodicals：Biochemical and Biophysical Research Communications

IF：2.62

【2017年】

Article：OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation

Periodicals：BioMed Research International

IF：2.583

【2016年】

Article：miR-3188 regulates nasopharyngeal carcinoma proliferation and chemosensitivity through a FOXO1-modulated positive feedback loop with mTOR–p-PI3K/AKT-c-JUN

Periodicals：NATURE COMMUNICATIONS

IF：12.353

Article：Long non-coding RNA LOC389641 promotes progression of pancreatic ductal adenocarcinoma and increases cell invasion by regulating E-cadherin in a TNFRSF10A-related manner

Periodicals：Cancer Letters

IF：6.491