实验原理：

针对目标区域设计生物素标记的特异性RNA探针偶联磁珠，并与细胞蛋白提取物孵育，蛋白分子与RNA探针特异性结合，经洗脱，得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。

 

产品优势：

1. 自主品牌：拥有自主知识产权专利。

2. 高品质，稳定性好。

3. 精准：特异性高，背景少。

4. 易用：操作简单，容易上手。

5. 快速省时。

实验流程：

结果实例：

详见产品说明书：Bes5102 RNA pulldown（12T、24T、40T）

文章案例

【2026年】

Article：Cannabidiol triggers fatty acids β-oxidation mediated by Stat2

Periodicals：Experimental & Molecular Medicine

IF：12.9

 

Article：Iron overload reprogramming lipid metabolism through the

Periodicals：Experimental & Molecular Medicine

IF：12.9

 

Article：Oxidative stress-driven m5 C methylation by NSUN5 confers cisplatin resistance

Periodicals：Redox Biology

IF：11.9

 

Article：The ANXA2P1-hnRNP F-HK2c-Myc Positive Feedback

Periodicals：International Journal of Biological Sciences

IF：9.9995

 

Article：NAT10 promotes cisplatin resistance and immune escape

Periodicals：Cell Death Discovery

IF：7.0

 

Article：The ZNF148 ZEB1 AS1 IGF2BP2 NOD2 Axis Drives Microglial Antipneumococcal Immunity

Periodicals：GLIA

IF：5.1000

ArArticle：CircROR1 binds HNRNPL to regulate FOXO4 pre-mRNA splicing, promoting cutaneous melanoma metastasis and serving as a therapeutic target via RNAi-loaded PEG-LNPs
Periodicals：Molecular Cancer
IF：33.8990

Article：Cannabidiol triggers fatty acids β-oxidation mediated by Stat2

Periodicals：Experimental & Molecular Medicine

IF：12.9

Article：Iron overload reprogramming lipid metabolism through the

Periodicals：Experimental & Molecular Medicine

IF：12.9

Article：Oxidative stress-driven m5 C methylation by NSUN5 confers cisplatin resistance

Periodicals：Redox Biology

IF：11.9

Article：DNMT2 inhibits anaplastic thyroid cancer progression by downregulating 5’tiRNAGly-GCC production
Periodicals：Cell Death & Disease
IF：9.5998

Article：Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate

Periodicals：Cellular and Molecular Life Sciences
IF：6.2001

 

【2025年】

Article：CircAKT3 promotes prostate cancer proliferation and metastasis by enhancing the binding of RPS27A and RPL11.

Periodicals：Molecular Cancer

IF：27.700

Article：PCBP2 Mediates Olaparib Resistance in Breast Cancer by Inhibiting m6A Methylation to Stabilize PARP1 mRNA.

Periodicals：Cancer Research

IF：16.600

Article： A novel feedback loop: CELF1/circ-CELF1/BRPF3/KAT7 in cardiac fibrosis. 

Periodicals：Acta Pharmceutica Sinica. B

IF：14.500

Article：ERβ/circAHNAK Axis Inhibits USP10-FMR1 Deubiquitination to Prevent m⁶A-Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma. 

Periodicals：Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

IF：14.100

Article：SUMOylation is a Translatable Target in Hypoxic MNPs Regulating Retinal Vasculopathy.

Periodicals：Advance Science (Weinheim, Baden-Wurttemberg, Germany)

IF：14.100

Article：Hsa_circ_0026782 Acts as a "Molecular Break" of CREB1-Mediated Transcription by Promoting Its Phosphorylation at Ser142 That Prevents Keloid Progression. 

Periodicals：Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

IF：14.100

Article： Genetic Regulation of Alternative Polyadenylation Provides Novel Insights into Molecular Mechanisms Underlying Non-small Cell Lung Cancer.

Periodicals：Advanced science (Weinheim, Baden-Wurttemberg, Germany)

IF：14.100

Article： Sperm-Derived CircRNA-1572 Regulates Embryogenesis and Zygotic Genome Activation by Targeting CCNB2 via Bta-miR-2478-L-2. 

Periodicals：Advanced science (Weinheim, Baden-Wurttemberg, Germany)

IF：14.100

Article： LncRNA TUG1 Repressed Angiogenesis by Promoting the Ubiquitination of HuR and Inhibiting Its Nuclear Translocation in Cerebral Ischemic Reperfusion Injury. 

Periodicals：Advanced science (Weinheim, Baden-Wurttemberg, Germany)

IF：14.100

Article：N6-methyladenosine-modified circDCP2 promotes carbon black nanoparticle-induced malignancy in human bronchial epithelial cells via PI3K-AKT pathway and macrophage homeostasis. 

Periodicals：Journal of Nanobiotechnology

IF：12.600

Article：RNA binding protein RBM20 regulates turtle temperature-dependent sex determination by repressing the splicing of Wt1 KTS.

Periodicals：Science Advances

IF：12.500

Article：Betaine inhibits the stem cell-like properties of hepatocellular carcinoma by activating autophagy via SAM/m6A/YTHDF1-mediated enhancement on ATG3 stability. 

Periodicals：Theranostics

IF：12.400

Article：AGD1/USP10/METTL13 complexes enhance cancer stem cells proliferation and diminish the therapeutic effect of docetaxel via CD44 m6A modification in castration resistant prostate cancer.

Periodicals：Journal of experimental & clinical cancer research: CR

IF：11.400

Article：piR-26441 inhibits mitochondrial oxidative phosphorylation and tumorigenesis in ovarian cancer through m6A modification by interacting with YTHDC1. 

Periodicals：Cell Death & Disease

IF：9.600

Article：M6A-METTL3-dependent nuclear PANC754/PSPC1/H3K4me1 repression complex regulate immune evasive LGALS7 signal to enhance immunotherapy against colorectal cancer.

Periodicals：Cell Death & Disease

IF：9.600

Article：LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver. 

Periodicals：Experimental & Molecular Medicine

IF：9.500

Article：CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling. 

Periodicals：Acta Pharmacologica Sinica

IF：8.400

Article：Targeting Zfp36 to combat cardiac hypertrophy: Insights into ferroptosis pathways. 

Periodicals：Clinical and Translational Medicine

IF：7.900

Article：METTL14-mediated m6A modification of ZFP14 inhibits clear cell renal cell carcinoma progression via promoting STAT3 ubiquitination. 

Periodicals：Clinical and Translational Medicine

IF：7.900

Article：Neutrophil extracellular traps promote growth of lung adenocarcinoma by mediating the stability of m6A-mediated SLC2A3 mRNA-induced ferroptosis resistance and CD8(+) T cell inhibition.

Periodicals：Clinical and Translational Medicine

IF：7.900

Article：MTA1-DT promotes endometrial cancer growth by modulating G2/M-related gene transcription via PURα. 

Periodicals：International Journal of Biological Macromolecules

IF：7.700

Article：CircSipa1l1 modulates melanoma cell differentiation by activating the IGF2BP1-ARHGDIB axis and ERK signaling pathway.

Periodicals：Journal of Translational Medicine

IF：7.500

Article：LincRNA-ASAO promotes dental pulp repair through interacting with PTBP1 to increase ALPL alternative splicing. 

Periodicals：Stem Cell Research & Therapy

IF：7.100

Article：Circular RNA circLIMK1-005 promotes the progression of lung adenocarcinoma by interacting with RPA1 protein to activate CDK4 signaling.

Periodicals：Cell Death Discovery

IF：7.000

Article：Urinary exosomal lnc-TAF12-2:1 promotes bladder cancer progression through the miR-7847-3p/ASB12 regulatory axis. 

Periodicals：Genes & Diseases

IF：6.900

Article：piRNA28846 has the potential to be a novel RNA nucleic acid drug for ovarian cancer.

Periodicals：NPJ Precision Oncology

IF：6.800

Article：Exosome circ-CBLB promotes M1 macrophage polarization in rheumatoid arthritis through the TLR3/TRAF3 signaling axis.

Periodicals：Frontiers in Immunology

IF：5.900

Article：WTAP modulates macrophage polarization in rheumatoid arthritis by targeting exosomal circ-CBLB via m6A modification. 

Periodicals：Frontiers in Immunology

IF：5.900

Article：MTHFD2 promotes esophageal squamous cell carcinoma progression via m6A modification‑mediated upregulation and modulation of the PEBP1‑RAF1 interaction.

Periodicals：International Journal of Molecular Medicine

IF：5.700

Article：A-to-I edited SNHG3 promotes non-small cell lung cancer metastasis by promoting fatty acid oxidation and resisting ferroptosis. 

Periodicals：Communications Biology

IF：5.100

Article：Exosome-derived hsa_circ_0007132 promotes lenvatinib resistance by inhibiting the ubiquitin-mediated degradation of NONO.

Periodicals：Non-coding RNA Research

IF：4.700

Article：Exosomal AFAP1-AS1 Promotes the Growth, Metastasis, and Glycolysis of Pituitary Adenoma by Inhibiting HuR Degradation.

Periodicals：Molecular Neurobiology

IF：4.600

Article：EIF4A3-Mediated circ_0008126 Inhibits the Progression and Metastasis of Gastric Cancer by Modulating the APC/β-Catenin Pathway. 

Periodicals：Cancers

IF：4.500

Article：AQP3 Influences Unexplained Recurrent Abortion by Regulating Trophoblast Cell Migration and Invasion via the METTL14/IGF2BP1/AQP3/PI3K/AKT Pathway.

Periodicals：Journal of Cellular and Molecular Medicine

IF：4.200

Article：LncRNA SNHG14 Regulated by ZNF460 Promotes Gastric Cancer Progression and Metastasis by Targeting the miR-206/FNDC3A Axis.

Periodicals：Journal of Cellular and Molecular Medicine

IF：4.200

Article：CircYAP1 inhibits osteosarcoma progression through the miR-135b-5p/FRK axis. 

Periodicals：iScience

IF：4.100

Article：Involvement of Mycobacterium smegmatis small noncoding RNA B11 in triacylglycerol accumulation and altered cell wall permeability. 

Periodicals：BMC Microbiology

IF：4.000

Article：METTL3-mediated m6A modification of LINC00857 enhances stemness and metastasis of ovarian cancer cells by activating the YAP-TEAD pathway. 

Periodicals：Scientific Reports

IF：3.900

Article：METTL3 alleviates renal tubular mitochondrial dysfunction by regulating the TUG1/PGC-1a axis in an IGF2BP2-dependent manner in diabetic nephropathy.

Periodicals：Renal Failure

IF：3.000

Article：Inhibition of LINC01048 decreases cell proliferation and induces the apoptosis in gastric cancer.

Periodicals：Discover Oncology

IF：2.900

【2024年】

Article：Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway.

Periodicals：Molecular cancer

IF：27.700

Article：An axonemal intron splicing program sustains Plasmodium male development.

Periodicals：Nature Communications

IF：14.700

Article：Positive feedback loop of c-myc/XTP6/NDH2/NF-κB to promote malignant progression in glioblastoma. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400

Article：FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400

Article：EIF4A3 modulated circ_000999 promotes epithelial-mesenchymal transition in cadmium-induced malignant transformation through the miR-205-5p/ZEB1 axis.

Periodicals：Environment International

IF：10.300

Article：Tumor-derived CCL15 regulates RNA m6A methylation in cancer-associated fibroblasts to promote hepatocellular carcinoma growth. 

Periodicals：Cancer Letters

IF：9.100

Article：CircUBE3A(2,3,4,5) promotes adenylate-uridylate-rich binding factor 1 nuclear translocation to suppress prostate cancer metastasis. 

Periodicals：Cancer Letters

IF：9.100

Article：Periodic changes of cyclin D1 mRNA stability are regulated by PC4 modifications in the cell cycle.

Periodicals：The Journal of Cell Biology

IF：7.400

Article：Urinary exosomal lnc-TAF12-2:1 promotes bladder cancer progression through the miR-7847-3p/ASB12 regulatory axis. 

Periodicals：Genes & Diseases

IF：6.900

Article：LSD1 deficiency in breast cancer cells promotes the formation of pre-metastatic niches

Periodicals：NPJ Precision Oncology

IF：6.800

Article：LSD1 modulates the bone metastasis of breast cancer cells through hnRNPA2B1-mediated sorting of exosomal miRNAs.

Periodicals：Cell death discovery

IF：6.100

Article：The long noncoding RNA ELFN1-AS1 promotes gastric cancer growth and metastasis by interacting with TAOK1 to inhibit the Hippo signaling pathway.

Periodicals：Cell death discovery

IF：6.100

Article：Long non-coding RNA LncTUG1 regulates favourable compression force-induced cementocytes mineralization via PU.1/TLR4/SphK1 signalling. 

Periodicals：Cell Proliferaiton

IF：5.900

Article：The pivotal role of ZNF384: driving the malignant behavior of serous ovarian cancer cells via the LIN28B/UBD axis.

Periodicals：Cell biology and toxicology

IF：5.300

Article：LINC02139 interacts with and stabilizes XIAP to regulate cell proliferation and apoptosis in gastric cancer. 

Periodicals：Communications Biology

IF：5.200

Article：CircSSR1 regulates pyroptosis of pulmonary artery smooth muscle cells through parental protein SSR1 mediating endoplasmic reticulum stress. 

Periodicals：Respiratory Research

IF：4.700

Article：IGF2BP2-m6A-circMMP9 axis recruits ETS1 to promote TRIM59 transcription in laryngeal squamous cell carcinoma. 

Periodicals：Scientific reports

IF：4.600

Article：Superenhancer-driven circRNA Myst4 involves in pulmonary artery smooth muscle cell ferroptosis in pulmonary hypertension.

Periodicals：iSicence

IF：4.600

Article：Bombinin-BO1 induces hepatocellular carcinoma cell-cycle arrest and apoptosis via the HSP90A-Cdc37-CDK1 axis. 

Periodicals：iSicence

IF：4.600

Article：Circ_0006476 modulates macrophage apoptosis through the miR-3074-5p/DLL4 axis: implications for Notch signalling pathway regulation in cardiovascular disease. 

Periodicals：Aging

IF：3.900

Article：EIF4A3-induced hsa_circ_0127071 promotes human glomerular mesangial cells senescence via JAK2/STAT5 signaling pathway. 

Periodicals：Scientific Reports

IF：3.800

Article：circNDUFA13 stimulates adipogenesis of bone marrow-derived mesenchymal stem cells via interaction with STAT3. 

Periodicals：Scientific Reports

IF：3.800

Article： The hnRNP A2B1 is important for the replication of SFTSV and other RNA viruses.

Periodicals：Microbiology Spectrum

IF：3.700

Article：circTADA2A inhibited SLC38A1 expression and suppresses melanoma progression through the prevention of CNBP trans-activation. 

Periodicals：PloS one

IF：3.700

Article： Circ_0087851 suppresses colorectal cancer malignant progression through triggering miR-593-3p/BAP1-mediated ferroptosis. 

Periodicals：Journal of cancer research and clinical oncology

IF：2.700

【2023年】

Article：CircRREB1 mediates lipid metabolism related senescent phenotypes in chondrocytes through FASN post-translational modifications

Periodicals：Nature Communications

IF：16.600

Article：Integrative Single-Cell Analysis Reveals Transcriptional and Epigenetic Regulatory Features of Clear Cell Renal Cell Carcinoma

Periodicals：Cancer Research

IF：11.200

Article：Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial-mesenchymal transition and angiogenesis

Periodicals：Clinical and Translational Medicine

IF：10.600

Article：Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration

Periodicals：Clinical and Translational Medicine

IF：10.600

Article：CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling

Periodicals：Translational Research

IF：10.17

Article：Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis

Periodicals：BMC Medicine

IF：9.300

Article： Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis

Periodicals：International Journal of Biological Sciences

IF：9.200

Article： A role of TRIM59 in pulmonary hypertension: modulating the protein ubiquitylation modification. J Transl Med

Periodicals：Journal of translational medicine

IF：6.100

Article：Nucleus-localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription

Periodicals：Cancer Science

IF：5.700

Article：Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes

Periodicals：International Journal of Molecular Sciences
IF：5.600

Article：SNHG25 promotes colorectal cancer metastasis by regulating MMP2.

Periodicals：Aging (Albany NY)
IF：5.200

Article：F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

Periodicals：Cancers

IF：5.200

Article：RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

Periodicals：International Journal of Oncology

IF：5.200

Article：FLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

Periodicals：Heliyon

IF：4.000

Article：LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter

Periodicals：BMC Cancer

IF：3.800

Article： IL-18 serves as a main effector of CAF-derived METTL3 against immunosuppression of NSCLC via driving NF-κB pathway.

Periodicals：Epigenetics

IF：3.700

【2022年】

Article：Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.300

Article：PHGDH Inhibits Ferroptosis and Promotes Malignant Progression by Upregulating SLC7A11 in Bladder Cancer

Periodicals：International Journal of Biological Sciences

IF：9.200

Article：LINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2

Periodicals：Cell Death and Disease

IF：9.865

Article：A reduced level of the long non-coding RNA SNHG8 activates the NF-kappaB pathway by releasing functional HIF-1alpha in a hypoxic infammatory microenvironment

Periodicals：Stem Cell Research & Therapy

IF：6.832

Article：LncRNA USP2-AS1 Promotes Hepatocellular Carcinoma Growth by Enhancing YBX1-Mediated HIF1a Protein Translation Under Hypoxia

Periodicals：Frontiers in Oncology

IF：6.243

Article：LINC00839 promotes malignancy of liver cancer via binding FMNL2 under hypoxia

Periodicals：Scientifc Reports

IF：4.996

【2021年】

Article：Super-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.16

Article：Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress

Periodicals：Theranostics

IF：8.579

Article：LINC-PINT impedes DNA repair and enhances radiotherapeutic response by targeting DNA-PKcs in nasopharyngeal cancer

Periodicals：Cell Death & Disease

IF：8.469

Article：LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma

Periodicals：Cell Death & Disease

IF：8.469

Article：LINC01133 promotes hepatocellular carcinoma progression by sponging miR-199a-5p and activating annexin A2

Periodicals：Clinical and Translational Medicine

IF：7.915

【2020年】

Article：Gut microbiota regulate tumor metastasis via circRNA/miRNA networks

Periodicals：Gut Microbes

IF：7.74

Article：Circular RNA 406961 interacts with ILF2 to regulate PM2.5-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways

Periodicals：Environment International

IF：7.577

【2019年】

Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

Periodicals：Advanced Science

IF：17.521

Article：Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

Periodicals：Molecular Cancer

IF：7.776

【2018年】

Article：Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN

Periodicals：The Journal of Clinical Investigation

IF：13.251

Article：Editor's Highlight: lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis

Periodicals：Society of Toxicology

IF：4.181