实验原理：

针对目标区域设计生物素标记的特异性RNA探针偶联磁珠，并与细胞蛋白提取物孵育，蛋白分子与RNA探针特异性结合，经洗脱，得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。

 

产品优势：

1. 自主品牌：拥有自主知识产权专利。

2. 高品质，稳定性好。

3. 精准：特异性高，背景少。

4. 易用：操作简单，容易上手。

5. 快速省时：全部实验只需3小时。

实验流程：

详见产品说明书：Bes5102 RNA pulldown

文章案例

【2024年】

Article：Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway.

Periodicals：Molecular cancer

IF：27.700

Article：An axonemal intron splicing program sustains Plasmodium male development.

Periodicals：Nature Communications

IF：14.700

Article：Positive feedback loop of c-myc/XTP6/NDH2/NF-κB to promote malignant progression in glioblastoma. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400

Article：FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400

Article：EIF4A3 modulated circ_000999 promotes epithelial-mesenchymal transition in cadmium-induced malignant transformation through the miR-205-5p/ZEB1 axis.

Periodicals：Environment International

IF：10.300

Article：Periodic changes of cyclin D1 mRNA stability are regulated by PC4 modifications in the cell cycle.

Periodicals：The Journal of Cell Biology

IF：7.400

Article：LSD1 modulates the bone metastasis of breast cancer cells through hnRNPA2B1-mediated sorting of exosomal miRNAs.

Periodicals：Cell death discovery

IF：6.100

Article：Long non-coding RNA LncTUG1 regulates favourable compression force-induced cementocytes mineralization via PU.1/TLR4/SphK1 signalling. 

Periodicals：Cell Proliferaiton

IF：5.900

Article：CircSSR1 regulates pyroptosis of pulmonary artery smooth muscle cells through parental protein SSR1 mediating endoplasmic reticulum stress. 

Periodicals：Respiratory Research

IF：4.700

Article：IGF2BP2-m6A-circMMP9 axis recruits ETS1 to promote TRIM59 transcription in laryngeal squamous cell carcinoma. 

Periodicals：Scientific reports

IF：4.600

Article：Superenhancer-driven circRNA Myst4 involves in pulmonary artery smooth muscle cell ferroptosis in pulmonary hypertension.

Periodicals：iSicence

IF：4.600

Article：Bombinin-BO1 induces hepatocellular carcinoma cell-cycle arrest and apoptosis via the HSP90A-Cdc37-CDK1 axis. 

Periodicals：iSicence

IF：4.600

Article：Circ_0006476 modulates macrophage apoptosis through the miR-3074-5p/DLL4 axis: implications for Notch signalling pathway regulation in cardiovascular disease. 

Periodicals：Aging

IF：3.900

Article：circNDUFA13 stimulates adipogenesis of bone marrow-derived mesenchymal stem cells via interaction with STAT3. 

Periodicals：Scientific Reports

IF：3.800

Article： The hnRNP A2B1 is important for the replication of SFTSV and other RNA viruses.

Periodicals：Microbiology Spectrum

IF：3.700

Article：circTADA2A inhibited SLC38A1 expression and suppresses melanoma progression through the prevention of CNBP trans-activation. 

Periodicals：PloS one

IF：3.700

Article： Circ_0087851 suppresses colorectal cancer malignant progression through triggering miR-593-3p/BAP1-mediated ferroptosis. 

Periodicals：Journal of cancer research and clinical oncology

IF：2.700

【2023年】

Article：CircRREB1 mediates lipid metabolism related senescent phenotypes in chondrocytes through FASN post-translational modifications

Periodicals：Nature Communications

IF：16.600

Article：Integrative Single-Cell Analysis Reveals Transcriptional and Epigenetic Regulatory Features of Clear Cell Renal Cell Carcinoma

Periodicals：Cancer Research

IF：11.200

Article：Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial-mesenchymal transition and angiogenesis

Periodicals：Clinical and Translational Medicine

IF：10.600

Article：Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration

Periodicals：Clinical and Translational Medicine

IF：10.600

Article：CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling

Periodicals：Translational Research

IF：10.17

Article：Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis

Periodicals：BMC Medicine

IF：9.300

Article： Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis

Periodicals：International Journal of Biological Sciences

IF：9.200

Article： A role of TRIM59 in pulmonary hypertension: modulating the protein ubiquitylation modification. J Transl Med

Periodicals：Journal of translational medicine

IF：6.100

Article：Nucleus-localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription

Periodicals：Cancer Science

IF：5.700

Article：Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes

Periodicals：International Journal of Molecular Sciences
IF：5.600

Article：SNHG25 promotes colorectal cancer metastasis by regulating MMP2.

Periodicals：Aging (Albany NY)
IF：5.200

Article：F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

Periodicals：Cancers

IF：5.200

Article：RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

Periodicals：International Journal of Oncology

IF：5.200

Article：FLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

Periodicals：Heliyon

IF：4.000

Article：LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter

Periodicals：BMC Cancer

IF：3.800

Article： IL-18 serves as a main effector of CAF-derived METTL3 against immunosuppression of NSCLC via driving NF-κB pathway.

Periodicals：Epigenetics

IF：3.700

【2022年】

Article：Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.300

Article：PHGDH Inhibits Ferroptosis and Promotes Malignant Progression by Upregulating SLC7A11 in Bladder Cancer

Periodicals：International Journal of Biological Sciences

IF：9.200

Article：LINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2

Periodicals：Cell Death and Disease

IF：9.865

Article：A reduced level of the long non-coding RNA SNHG8 activates the NF-kappaB pathway by releasing functional HIF-1alpha in a hypoxic infammatory microenvironment

Periodicals：Stem Cell Research & Therapy

IF：6.832

Article：LncRNA USP2-AS1 Promotes Hepatocellular Carcinoma Growth by Enhancing YBX1-Mediated HIF1a Protein Translation Under Hypoxia

Periodicals：Frontiers in Oncology

IF：6.243

Article：LINC00839 promotes malignancy of liver cancer via binding FMNL2 under hypoxia

Periodicals：Scientifc Reports

IF：4.996

【2021年】

Article：Super-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.16

Article：Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress

Periodicals：Theranostics

IF：8.579

Article：LINC-PINT impedes DNA repair and enhances radiotherapeutic response by targeting DNA-PKcs in nasopharyngeal cancer

Periodicals：Cell Death & Disease

IF：8.469

Article：LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma

Periodicals：Cell Death & Disease

IF：8.469

Article：LINC01133 promotes hepatocellular carcinoma progression by sponging miR-199a-5p and activating annexin A2

Periodicals：Clinical and Translational Medicine

IF：7.915

Article：CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs

Periodicals：Cell Death & Disease

IF：6.304

【2020年】

Article：Gut microbiota regulate tumor metastasis via circRNA/miRNA networks

Periodicals：Gut Microbes

IF：7.74

Article：Circular RNA 406961 interacts with ILF2 to regulate PM2.5-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways

Periodicals：Environment International

IF：7.577

【2019年】

Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

Periodicals：Advanced Science

IF：17.521

Article：Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

Periodicals：Molecular Cancer

IF：7.776

【2018年】

Article：Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN

Periodicals：The Journal of Clinical Investigation

IF：13.251

Article：Editor's Highlight: lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis

Periodicals：Society of Toxicology

IF：4.181