蛋白质与RNA的相互作用是许多细胞生物学过程的核心,如蛋白质合成、mRNA 组装、病毒复制、细胞发育调控等,研究它们之间相互作用的分子机制对理解这些生物学过程非常重要。RNA pulldown技术通过体外转录标记生物素的RNA探针耦联链霉亲和素标记的磁珠,实现了对RNA结合蛋白质(RBPs)的高效富集和鉴定。 (本试剂盒仅供科研用途)
实验原理:
针对目标区域设计生物素标记的特异性RNA探针偶联磁珠,并与细胞蛋白提取物孵育,蛋白分子与RNA探针特异性结合,经洗脱,得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。
产品优势:
1. 自主品牌:拥有自主知识产权专利。
2. 高品质,稳定性好。
3. 精准:特异性高,背景少。
4. 易用:操作简单,容易上手。
5. 快速省时:全部实验只需3小时。
实验流程:
文章案例
【2024年】
Periodicals:Molecular cancer
IF:27.700
Article:An axonemal intron splicing program sustains Plasmodium male development.
Periodicals:Nature Communications
IF:14.700
Article:Positive feedback loop of c-myc/XTP6/NDH2/NF-κB to promote malignant progression in glioblastoma.
Periodicals:Journal of experimental & clinical cancer research
IF:11.400
Periodicals:Journal of experimental & clinical cancer research
IF:11.400
Periodicals:Environment International
IF:10.300
Article:Periodic changes of cyclin D1 mRNA stability are regulated by PC4 modifications in the cell cycle.
Periodicals:The Journal of Cell Biology
IF:7.400
Periodicals:Cell death discovery
IF:6.100
Periodicals:Cell Proliferaiton
IF:5.900
Periodicals:Respiratory Research
IF:4.700
Periodicals:Scientific reports
IF:4.600
Periodicals:iSicence
IF:4.600
Periodicals:iSicence
IF:4.600
Periodicals:Aging
IF:3.900
Periodicals:Scientific Reports
IF:3.800
Article: The hnRNP A2B1 is important for the replication of SFTSV and other RNA viruses.
Periodicals:Microbiology Spectrum
IF:3.700
Periodicals:PloS one
IF:3.700
Periodicals:Journal of cancer research and clinical oncology
IF:2.700
【2023年】
Periodicals:Nature Communications
IF:16.600
Periodicals:Cancer Research
IF:11.200
Periodicals:Clinical and Translational Medicine
IF:10.600
Periodicals:Clinical and Translational Medicine
IF:10.600
Periodicals:Translational Research
IF:10.17
Periodicals:BMC Medicine
IF:9.300
Periodicals:International Journal of Biological Sciences
IF:9.200
Periodicals:Journal of translational medicine
IF:6.100
Periodicals:Cancer Science
IF:5.700
Periodicals:International Journal of Molecular Sciences
IF:5.600
Article:SNHG25 promotes colorectal cancer metastasis by regulating MMP2.
Periodicals:Aging (Albany NY)
IF:5.200
Periodicals:Cancers
IF:5.200
Periodicals:International Journal of Oncology
IF:5.200
Periodicals:Heliyon
IF:4.000
Periodicals:BMC Cancer
IF:3.800
Periodicals:Epigenetics
IF:3.700
【2022年】
Periodicals:Journal of Experimental & Clinical Cancer Research
IF:11.300
Periodicals:International Journal of Biological Sciences
IF:9.200
Periodicals:Cell Death and Disease
IF:9.865
Periodicals:Stem Cell Research & Therapy
IF:6.832
Periodicals:Frontiers in Oncology
IF:6.243
Article:LINC00839 promotes malignancy of liver cancer via binding FMNL2 under hypoxia
Periodicals:Scientifc Reports
IF:4.996
【2021年】
Periodicals:Journal of Experimental & Clinical Cancer Research
IF:11.16
Article:Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress
Periodicals:Theranostics
IF:8.579
Periodicals:Cell Death & Disease
IF:8.469
Periodicals:Cell Death & Disease
IF:8.469
Periodicals:Clinical and Translational Medicine
IF:7.915
Periodicals:Cell Death & Disease
IF:6.304
【2020年】
Article:Gut microbiota regulate tumor metastasis via circRNA/miRNA networks
Periodicals:Gut Microbes
IF:7.74
Periodicals:Environment International
IF:7.577
【2019年】
Periodicals:Advanced Science
IF:17.521
Periodicals:Molecular Cancer
IF:7.776
【2018年】
Article:Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN
Periodicals:The Journal of Clinical Investigation
IF:13.251
Periodicals:Society of Toxicology
IF:4.181