RNA pulldown试剂盒
实验介绍

蛋白质与RNA的相互作用是许多细胞生物学过程的核心,如蛋白质合成、mRNA 组装、病毒复制、细胞发育调控等,研究它们之间相互作用的分子机制对理解这些生物学过程非常重要。RNA pulldown技术通过体外转录标记生物素的RNA探针耦联链霉亲和素标记的磁珠,实现了对RNA结合蛋白质(RBPs)的高效富集和鉴定。 (本试剂盒仅供科研用途)

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详细介绍


实验原理:


针对目标区域设计生物素标记的特异性RNA探针偶联磁珠,并与细胞蛋白提取物孵育,蛋白分子与RNA探针特异性结合,经洗脱,得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。

 

产品优势:


1. 自主品牌:拥有自主知识产权专利。

2. 高品质,稳定性好。

3. 精准:特异性高,背景少。

4. 易用:操作简单,容易上手。

5. 快速省时:全部实验只需3小时。


实验流程:





详见产品说明书Bes5102 RNA pulldown



文章案例



2023


ArticleCircRREB1 mediates lipid metabolism related senescent phenotypes in chondrocytes through FASN post-translational modifications

Periodicals:Nature Communications

IF16.600


ArticleIntegrative Single-Cell Analysis Reveals Transcriptional and Epigenetic Regulatory Features of Clear Cell Renal Cell Carcinoma

PeriodicalsCancer Research

IF11.200


ArticleUpregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial-mesenchymal transition and angiogenesis

Periodicals:Clinical and Translational Medicine

IF:10.600


ArticleCircular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration

PeriodicalsClinical and Translational Medicine

IF10.600


ArticleCircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling

PeriodicalsTranslational Research

IF10.17


ArticleDownregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis

PeriodicalsBMC Medicine

IF:9.300


Article Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis

Periodicals:International Journal of Biological Sciences

IF9.200


ArticleNucleus-localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription

Periodicals:Cancer Science

IF:5.700


Article:Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes

Periodicals:International Journal of Molecular Sciences
IF:5.600


Article:SNHG25 promotes colorectal cancer metastasis by regulating MMP2.

Periodicals:Aging (Albany NY)
IF:5.200


ArticleF-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

PeriodicalsCancers

IF:5.200


ArticleRBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

Periodicals:International Journal of Oncology

IF:5.200


ArticleFLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

Periodicals:Heliyon

IF:4.000


ArticleLINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter

PeriodicalsBMC Cancer

IF:3.800


Article IL-18 serves as a main effector of CAF-derived METTL3 against immunosuppression of NSCLC via driving NF-κB pathway.

PeriodicalsEpigenetics

IF:3.700



2022


ArticleLong non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer

PeriodicalsJournal of Experimental & Clinical Cancer Research

IF11.300


ArticlePHGDH Inhibits Ferroptosis and Promotes Malignant Progression by Upregulating SLC7A11 in Bladder Cancer

PeriodicalsInternational Journal of Biological Sciences

IF:9.200


ArticleLINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2

PeriodicalsCell Death and Disease

IF9.865


ArticleA reduced level of the long non-coding RNA SNHG8 activates the NF-kappaB pathway by releasing functional HIF-1alpha in a hypoxic infammatory microenvironment

PeriodicalsStem Cell Research & Therapy

IF6.832


ArticleLncRNA USP2-AS1 Promotes Hepatocellular Carcinoma Growth by Enhancing YBX1-Mediated HIF1a Protein Translation Under Hypoxia

PeriodicalsFrontiers in Oncology

IF6.243


ArticleLINC00839 promotes malignancy of liver cancer via binding FMNL2 under hypoxia

PeriodicalsScientifc Reports

IF4.996



2021


ArticleSuper-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc

PeriodicalsJournal of Experimental & Clinical Cancer Research

IF11.16


Article:Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress

Periodicals:Theranostics

IF:8.579


ArticleLINC-PINT impedes DNA repair and enhances radiotherapeutic response by targeting DNA-PKcs in nasopharyngeal cancer

PeriodicalsCell Death & Disease

IF8.469


Article:LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma

Periodicals:Cell Death & Disease

IF:8.469


ArticleLINC01133 promotes hepatocellular carcinoma progression by sponging miR-199a-5p and activating annexin A2

PeriodicalsClinical and Translational Medicine

IF7.915


ArticleCircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs

PeriodicalsCell Death & Disease

IF6.304



2020


ArticleGut microbiota regulate tumor metastasis via circRNA/miRNA networks

PeriodicalsGut Microbes

IF7.74


Article:Circular RNA 406961 interacts with ILF2 to regulate PM2.5-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways

Periodicals:Environment International

IF:7.577



2019


Article:Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

PeriodicalsAdvanced Science

IF17.521


ArticleCircular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

PeriodicalsMolecular Cancer

IF7.776



2018


ArticleLong noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN

PeriodicalsThe Journal of Clinical Investigation

IF13.251


ArticleEditor's Highlight: lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis

PeriodicalsSociety of Toxicology

IF4.181

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