蛋白质与RNA的相互作用是许多细胞功能的核心，如蛋白质合成、mRNA组装、病毒复制、细胞发育调控等，了解它们之间相互作用的分子机制对理解这些生物学过程非常重要。RNA pulldown技术利用脱硫生物素末端标记的RNA和链霉亲和素标记的磁珠高效富集及鉴定RNA结合蛋白质（RBPs）。

      针对目标区域设计脱硫生物素标记的特异性RNA探针，并与细胞蛋白提取物孵育。通过偶联磁珠，经洗脱，得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。

产品特点

1. 精准：高品质，稳定性好；

2. 易用：操作简单，容易上手；

3. 背景低：特异性高，背景少；

4. 自主品牌：拥有自主知识产权专利；

5. 快速省时：全部实验只需3小时

实验流程

详见产品说明书：Bes5102 RNA pulldown

文章案例

Article：CircSERPINE2 protects against osteoarthritis by targeting miR-1271 and ETS-related gene

Periodicals：Annals of the Rheumatic Diseases

IF：14.299

 

Article：Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN

Periodicals：The Journal of Clinical Investigation

IF：13.251

 

Article：Super-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.16

 

Article：Hsa_circ_0004296 inhibits metastasis of prostate cancer by interacting with EIF4A3 to prevent nuclear export of ETS1 mRNA

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.16

 

Article：hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3

Periodicals：Molecular Therapy-Nucleic Acids

IF：8.886

Article：Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer

Periodicals：Cancer Letters

IF：8.697

Article：Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress

Periodicals：Theranostics

IF：8.579

Article：N6-methyladenosine-induced circ1662 promotes metastasis of colorectal cancer by accelerating YAP1 nuclear localization

Periodicals：Theranostics

IF：8.579

Article：A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/Prc1 (and Nuf2) axis

Periodicals：Cell Death & Disease

IF：8.469

Article：LINC-PINT impedes DNA repair and enhances radiotherapeutic response by targeting DNA-PKcs in nasopharyngeal cancer

Periodicals：Cell Death & Disease

IF：8.469

Article：LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma

Periodicals：Cell Death & Disease

IF：8.469

Article：Circular RNA circRNA_000203 aggravates cardiac hypertrophy via suppressing miR-26b-5p and miR-140-3p binding to Gata4

Periodicals：Cardiovascular Research

IF：8.168

Article：LINC01133 promotes hepatocellular carcinoma progression by sponging miR-199a-5p and activating annexin A2

Periodicals：Clinical and Translational Medicine

IF：7.915

Article：Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

Periodicals：Molecular Cancer

IF：7.776

Article：Gut microbiota regulate tumor metastasis via circRNA/miRNA networks

Periodicals：Gut Microbes

IF：7.74

Article：Circular RNA 406961 interacts with ILF2 to regulate PM2.5-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways

Periodicals：Environment International

IF：7.577

Article：circCAMSAP1 promotes osteosarcoma progression and metastasis by sponging miR-145-5p and regulating FLI1 expression

Periodicals：Molecular Therapy: Nucleic Acids

IF：7.032

Article：Hsa_circ_0000652 Aggravates Inflammation by Activation of Macrophages and Enhancement of OX40/OX40L Interaction in Ankylosing Spondylitis

Periodicals：Molecular and Cellular Pathology

IF：6.68

Article：CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs

Periodicals：Cell Death & Disease

IF：6.304

Article：Angiogenesis is Inhibited by Arsenic Trioxide Through Downregulation of the CircHIPK3/miR-149-5p/FOXO1/VEGF Functional Module in Rheumatoid Arthritis

Periodicals：Frontiers in Pharmacology

IF：5.8

Article：CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer

Periodicals：Cell Death Discovery 

IF：5.24

Article：Hsa_circ_0006571 promotes spinal metastasis through sponging microRNA-138 to regulate sirtuin 1 expression in lung adenocarcinoma

Periodicals：Translational Lung Cancer Research

IF：5.132

Article：Editor's Highlight: lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis

Periodicals：Society of Toxicology

IF：4.181

Article：CircHIPK3 Facilitates the G2/M Transition in Prostate Cancer Cells by Sponging miR-338-3p

Periodicals：OncoTargets and Therapy

IF：3.259

Article：Circular RNA FOXO3 accelerates glycolysis and improves cisplatin sensitivity in lung cancer cells via the miR‑543/Foxo3 axis

Periodicals：Oncology Letters

IF：2.967