
蛋白质与RNA的相互作用是许多细胞功能的核心,如蛋白质合成、mRNA组装、病毒复制、细胞发育调控等,了解它们之间相互作用的分子机制对理解这些生物学过程非常重要。RNA pulldown技术利用脱硫生物素末端标记的RNA和链霉亲和素标记的磁珠高效富集及鉴定RNA结合蛋白质(RBPs)。 针对目标区域设计脱硫生物素标记的特异性RNA探针,并与细胞蛋白提取物孵育。通过偶联磁珠,经洗脱,得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。 (本试剂盒仅供科研用途)
蛋白质与RNA的相互作用是许多细胞功能的核心,如蛋白质合成、mRNA组装、病毒复制、细胞发育调控等,了解它们之间相互作用的分子机制对理解这些生物学过程非常重要。RNA pulldown技术利用脱硫生物素末端标记的RNA和链霉亲和素标记的磁珠高效富集及鉴定RNA结合蛋白质(RBPs)。
针对目标区域设计脱硫生物素标记的特异性RNA探针,并与细胞蛋白提取物孵育。通过偶联磁珠,经洗脱,得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。
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文章案例
Article:CircSERPINE2 protects against osteoarthritis by targeting miR-1271 and ETS-related gene
Periodicals:Annals of the Rheumatic Diseases
IF:14.299
Article:Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN
Periodicals:The Journal of Clinical Investigation
IF:13.251
Periodicals:Journal of Experimental & Clinical Cancer Research
IF:11.16
Periodicals:Journal of Experimental & Clinical Cancer Research
IF:11.16
Article:hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3
Periodicals:Molecular Therapy-Nucleic Acids
IF:8.886
Article:Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer
Periodicals:Cancer Letters
IF:8.697
Article:Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress
Periodicals:Theranostics
IF:8.579
Periodicals:Theranostics
IF:8.579
Periodicals:Cell Death & Disease
IF:8.469
Periodicals:Cell Death & Disease
IF:8.469
Periodicals:Cell Death & Disease
IF:8.469
Periodicals:Cardiovascular Research
IF:8.168
Periodicals:Clinical and Translational Medicine
IF:7.915
Periodicals:Molecular Cancer
IF:7.776
Article:Gut microbiota regulate tumor metastasis via circRNA/miRNA networks
Periodicals:Gut Microbes
IF:7.74
Periodicals:Environment International
IF:7.577
Periodicals:Molecular Therapy: Nucleic Acids
IF:7.032
Periodicals:Molecular and Cellular Pathology
IF:6.68
Periodicals:Cell Death & Disease
IF:6.304
Periodicals:Frontiers in Pharmacology
IF:5.8
Article:CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer
Periodicals:Cell Death Discovery
IF:5.24
Periodicals:Translational Lung Cancer Research
IF:5.132
Periodicals:Society of Toxicology
IF:4.181
Article:CircHIPK3 Facilitates the G2/M Transition in Prostate Cancer Cells by Sponging miR-338-3p
Periodicals:OncoTargets and Therapy
IF:3.259
Periodicals:Oncology Letters
IF:2.967