RNA pulldown试剂盒
实验介绍

蛋白质与RNA的相互作用是许多细胞功能的核心,如蛋白质合成、mRNA组装、病毒复制、细胞发育调控等,了解它们之间相互作用的分子机制对理解这些生物学过程非常重要。RNA pulldown技术利用脱硫生物素末端标记的RNA和链霉亲和素标记的磁珠高效富集及鉴定RNA结合蛋白质(RBPs)。 针对目标区域设计脱硫生物素标记的特异性RNA探针,并与细胞蛋白提取物孵育。通过偶联磁珠,经洗脱,得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。 (本试剂盒仅供科研用途)

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详细介绍

      蛋白质与RNA的相互作用是许多细胞功能的核心,如蛋白质合成、mRNA组装、病毒复制、细胞发育调控等,了解它们之间相互作用的分子机制对理解这些生物学过程非常重要。RNA pulldown技术利用脱硫生物素末端标记的RNA和链霉亲和素标记的磁珠高效富集及鉴定RNA结合蛋白质(RBPs)。

      针对目标区域设计脱硫生物素标记的特异性RNA探针,并与细胞蛋白提取物孵育。通过偶联磁珠,经洗脱,得到目的RNA探针-蛋白质复合物。最后采用Western Blot或质谱鉴定蛋白质类型。


产品特点

1. 精准:高品质,稳定性好;

2. 易用:操作简单,容易上手;

3. 背景低:特异性高,背景少;

4. 自主品牌:拥有自主知识产权专利;

5. 快速省时:全部实验只需3小时


实验流程






详见产品说明书Bes5102 RNA pulldown





文章案例


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