染色质免疫沉淀 (ChIP) 是研究体内蛋白质与 DNA 相互作用的理想技术，是目前确定与特定蛋白结合的基因组区域，或确定与特定基因组区域结合的蛋白质的最好方法。ChIP 与其他实验技术相结合，可用于不同用途的检测。ChIP-Seq 方法是对特异性地富集目的蛋白结合的 DNA 片段进行纯化与文库构建，然后对富集得到的 DNA 片段进行高通量测序。

      在活细胞状态下把细胞内的蛋白质和 DNA 交联，超声波将其随机切断为一定长度范围内的染色质小片段，然后用所研究的目的蛋白质特异性抗体免疫沉淀蛋白质-DNA 复合体，从而特异性地富集目的蛋白结合的 DNA 片段。

产品特点

1. 高效及特异的富集效率；阳性和阴性对照的富集比率>120，每个ChIP反应只需要100,000个细胞。

2. 对下游分析方法的兼容性强，可兼容的下游分析方法包括：RT-qPCR、基因芯片和高通量测序等。

3. 优秀的技术服务团队，技术服务高效、全面、迅速，能及时帮助客户尽快解决实验中遇到的问题。

技术流程

结果实例

详见产品说明书：Bes5001 ChIP（动物）

详见产品说明书：Bes5002 ChIP（植物）

文章案例

Article：STAT3-NAV2 axis as a new therapeutic target for rheumatoid arthritis via activating SSH1L/Cofilin-1 signaling pathway

Periodicals：Signal Transduction and Targeted Therapy

IF：38.104

Article：N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer

Periodicals：Nature Communications

IF：17.694

Article：Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells

Periodicals：Science Advances

IF：14.142

Article：Transcriptional upregulation of microtubule-associated protein 2 is involved in the protein kinase A-induced decrease in the invasiveness of glioma cells

Periodicals：Neuro-Oncology

IF：9.384

Article：Dual immunological and proliferative regulation of immune checkpoint FGL1 in lung adenocarcinoma: The pivotal role of the YY1–FGL1–MYH9 axis

Periodicals：Frontiers in Immunology

IF：8.786

Article：LncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity

Periodicals：Cell Death & Disease

IF：8.469

Article：NAV2 positively modulates inflammatory response of fibroblast-like synoviocytes through activating Wnt/β-catenin signaling pathway in rheumatoid arthritis

Periodicals：Clinical and Translational Medicine

IF：7.919

Article：Interaction between endogenous microRNAs and virus-derived small RNAs controls viral replication in insect vectors

Periodicals：PLOS Pathogens

IF：7.455

Article：Sp1 S-Sulfhydration Induced by Hydrogen Sulfide Inhibits Inflammation via HDAC6/MyD88/NF-κB Signaling Pathway in Adjuvant-Induced Arthritis

Periodicals：Antioxidants

IF：6.313

Article：RIF1 promotes human epithelial ovarian cancer growth and progression via activating human telomerase reverse transcriptase expression

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：6.217

Article：An insertion variant of MGMT disrupts a STAT1 binding site and confers susceptibility to glioma

Periodicals：Cancer Cell International

IF：5.722

Article：LncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1

Periodicals：Journal of Cellular and Molecular Medicine

IF：4.302

Article：SLC2A3 promotes macrophage infltration by glycolysis reprogramming in gastric cancer

Periodicals：Cancer Cell International

IF：4.175

Article：Nrf2-regulated miR-380-3p blocks the translation of Sp3 protein and its mediation of paraquat-induced toxicity in mouse neuroblastoma N2a cells

Periodicals：Toxicological Sciences

IF：3.829

Article：Shortened nuclear matrix attachment regions are sufficient for replication and maintenance of episomes in mammalian cells

Periodicals：Molecular Biology of the Cell

IF：3.791

Article：Genetic Predisposition to Glioma Mediated by a MAPKAP1 Enhancer Variant

Periodicals：Cellular and Molecular Neurobiology

IF：3.606