ChIP试剂盒
实验介绍

染色质免疫沉淀 (ChIP) 是研究体内蛋白质与 DNA 相互作用的理想技术,是目前确定与特定蛋白结合的基因组区域,或确定与特定基因组区域结合的蛋白质的最好方法。 (本试剂盒仅供科研用途)

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详细介绍

      染色质免疫沉淀 (ChIP) 是研究体内蛋白质与 DNA 相互作用的理想技术,是目前确定与特定蛋白结合的基因组区域,或确定与特定基因组区域结合的蛋白质的最好方法。ChIP 与其他实验技术相结合,可用于不同用途的检测。ChIP-Seq 方法是对特异性地富集目的蛋白结合的 DNA 片段进行纯化与文库构建,然后对富集得到的 DNA 片段进行高通量测序。

      在活细胞状态下把细胞内的蛋白质和 DNA 交联,超声波将其随机切断为一定长度范围内的染色质小片段,然后用所研究的目的蛋白质特异性抗体免疫沉淀蛋白质-DNA 复合体,从而特异性地富集目的蛋白结合的 DNA 片段。


产品特点

1. 高效及特异的富集效率;阳性和阴性对照的富集比率>120,每个ChIP反应只需要100,000个细胞。

2. 对下游分析方法的兼容性强,可兼容的下游分析方法包括:RT-qPCR、基因芯片和高通量测序等。

3. 优秀的技术服务团队,技术服务高效、全面、迅速,能及时帮助客户尽快解决实验中遇到的问题。


技术流程


结果实例





详见产品说明书Bes5001 ChIP(动物)

详见产品说明书Bes5002 ChIP(植物)





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