实验原理：

在活细胞状态下使细胞内的蛋白质和DNA交联，并用超声波将其随机切断为一定长度范围内的染色质小片段。选择与目的蛋白特异性结合的抗体免疫沉淀DNA-蛋白质复合体，从而特异性地富集与目的蛋白结合的染色体片段。

产品优势：

1. 高效及特异的富集效率；阳性和阴性对照的富集比率>120。

2. 对下游分析方法的兼容性强，可兼容RT-qPCR、基因芯片和高通量测序等。

3. 优秀的技术服务团队，技术服务高效、全面、迅速，能及时帮助客户尽快解决实验中遇到的问题。

技术流程：

 

结果实例：

 

详见产品说明书：Bes5001 ChIP（动物）

详见产品说明书：Bes5002 ChIP（植物）

文章案例

【2026年】

Article：TGM2 Aggravates Acute Pancreatitis by Impairing Macrophage Efferocytosis

Periodicals：Advanced Science

IF：14.1

 

Article：Podocyte TLR4 deletion alleviates diabetic kidney disease through prohibiting

Periodicals：Journal of Advanced Research

IF：12.9994

 

Article：Single-cell RNA-seq and in vitro study reveal Fusobacterium nucleatum impairs

Periodicals：Journal of Translational Medicine

IF：7.4996

 

Article：RUNX1 restrains STAT1-GITRL signaling to shape an immunosuppressive CRC

Periodicals：Cell Death Discovery

IF：6.9997

 

Article：Clusterin elaborated by renal tubular epithelial cells under high

Periodicals：Cellular and Molecular Life Sciences

IF：6.2001

 

Article：Low-dose Simvastatin protects pancreatic cancer cells by promoting

Periodicals：Cellular Oncology

IF：4.8003

Article：M6A modification mediates CACNA1A stability to drive the progression of ovarian cancer by inhibiting ferroptosis
Periodicals：Journal of Ovarian Research
IF：4.1998

Article：Hypoxic mesenchymal stem cell-Derived Exosomal circPTP4A2 improves granulosa cell mitochondrial function via YBX1
Periodicals：Cellular and Molecular Life Sciences
IF：6.2001

Article：CSNK1D inhibition suppresses head and neck squamous cell carcinoma progression through SHH and PTCH1 pathway
Periodicals：Cell Death & Disease
IF：9.5998

【2025年】

Article： GRPR-induced FAM135A expression promote perineural invasion in prostate cancer. 

Periodicals：Molecular Cancer

IF：33.900

Article：PRODH2-Mediated Metabolism in the Bone Microenvironment Promotes Breast Cancer Metastasis. 

Periodicals：Cancer Research

IF：16.600

Article： Long-chain acylcarnitine deficiency promotes hepatocarcinogenesis.

Periodicals：Acta pharmaceutica Sinica. B

IF：14.700

Article： A glucose-enriched lung pre-metastatic niche triggered by matrix stiffness-tuned exosomal miRNAs in hepatocellular carcinoma.

Periodicals：Nature Communications

IF：14.700

Article：FGL1-mediated lymph node metastasis in stage T1 non-small cell lung cancer: therapeutic targeting.

Periodicals：Experimental Hematology & Oncology

IF：13.500

Article： Eriodictyol-cisplatin coated nanomedicine synergistically promote osteosarcoma cells ferroptosis and chemosensitivity.

Periodicals：Journal of Nanobiotechnology

IF：12.600

Article：Targeting HMGB2 acts as dual immunomodulator by bolstering CD8+ T cell function and inhibiting tumor growth in hepatocellular carcinoma. 

Periodicals：Science Advances

IF：12.500

Article：o8G-modified circKIAA1797 promotes lung cancer development by inhibiting cuproptosis. 

Periodicals：Journal of Experimental & Clinincal Cancer Research: CR

IF：11.400

Article：C-FOS promotes the formation of neutrophil extracellular traps and the recruitment of neutrophils in lung metastasis of triple-negative breast cancer. 

Periodicals：Journal of Experimental & Clinical Cancer Research: CR

IF：11.400

Article：tRF3a-MetCAT Promotes EGFR-Targeted Therapeutic Resistance through the TRIM21-STAT1-C5a Axis in Lung Adenocarcinoma. 

Periodicals：Research (Washington,D.C.)

IF：10.700

Article：Degradation of IRF6 by TRIM59 in tumor cells triggers PGM1-mediated glycolysis to regulate cell proliferation in neuroblastoma.

Periodicals：Cell Death & Disease

IF：9.600

Article：Ferroptosis mediated by the IDO1/Kyn/AhR pathway triggers acute thymic involution in sepsis. 

Periodicals：Cell Death & Disease

IF：9.600

Article：Chemotherapy-induced macrophage CXCL7 expression drives tumor chemoresistance via the STAT1/PHGDH-serine metabolism axis and SAM paracrine feedback to M2 polarization. 

Periodicals：Cell Death & Disease

IF：9.600

Article：Mitochondrial ribosomal protein L12 mediates metabolic reorganization in clear cell renal cell carcinoma by regulating mitochondrial biosynthesis. 

Periodicals：Cell Communication and Signaling : CCS

IF：8.900

Article：TFE3 fusion proteins promote the progression of TFE3 rearranged renal cell carcinoma via enhancing chaperone-mediated lipophagy. 

Periodicals：Cell Communication and Signaling: CCS

IF：8.200

Article：MTA1-DT promotes endometrial cancer growth by modulating G2/M-related gene transcription via PURα. 

Periodicals：International Journal of Biological Macromolecules

IF：7.700

Article：ELF3-regulated PES1 targets VEGFR2 to mediate angiogenesis and retinal inner barrier injury in diabetic retinopathy.

Periodicals：Journal of Translational Medicine

IF：7.500

Article：CBX7 regulates chemotherapy-induced senescence-like growth arrest in multiple myeloma via the ERK/STAT3/PIM1 axis. 

Periodicals：Journal of Translational Medicine

IF：7.500

Article：USP21/YBX1/HIF1-α promotes the progression of prostate cancer.

Periodicals：Journal of Translational Medicine

IF：7.500

Article：KPNA2 promotes osteosarcoma progression by regulating the alternative splicing of DDX3X mediated by YBX1.

Periodicals：Oncogene

IF：7.300

Article：MSC-exosomes pretreated by Danshensu extracts pretreating to target the hsa-miR-27a-5p and STAT3-SHANK2 to enhanced antifibrotic therapy.

Periodicals：Stem Cell Research & Therapy

IF：7.100

Article：TEAD4-mediated upregulation of LPAR3 augments hepatic stellate cell activation in portal hypertension.

Periodicals：Cell Biology and Toxicology

IF：5.900

Article：Mechanisms of sorafenib-induced cardiotoxicity: ER stress induces upregulation of ATF3, leading to downregulation of NDUFS1 expression and mitochondrial dysfunction.

Periodicals：Frontiers in Pharmacology

IF：4.800

Article：PRDX2 promotes gastric cancer progression by forming a feedback loop with PKM2/STAT3 axis.

Periodicals：Cellular Signalling

IF：4.400

Article：TFAP2A-regulated CRNDE enhances colon cancer progression and chemoresistance via RIPK3 interaction.

Periodicals：Functional & Integrative Genomics

IF：3.900

Article： IL15RA-STAT3-GPX4/ACSL3 signaling leads to ferroptosis resistance in pancreatic cancer. 

Periodicals：Acta Biochimical et Biophysica Sinica

IF：3.300

【2024年】

Article：The circUbqln1, regulated by XBP1s, interplays with 14-3-3ζ to inhibit collagen synthesis and promote osteoarthritis by controlling PRODH activity and proline metabolism. 

Periodicals：Journal of Advanced Research

IF：11.400

Article：Evaluating the Effects of Perinatal Exposures to BPSIP on Hepatic Cholesterol Metabolism in Female and Male Offspring ICR Mice. 

Periodicals：Enviromental Health Perspectives

IF：10.100

Article：Unveiling the mechanism of broad-spectrum blast resistance in rice: The collaborative role of transcription factor OsGRAS30 and histone deacetylase OsHDAC1.

Periodicals：Plant biotechnology journal

IF：10.100

Article：FTO-mediated SMAD2 m6A modification protects cartilage against Osteoarthritis.

Periodicals：Experimental & Molecular Medicine

IF：9.500

Article： Endothelial β-catenin upregulation and Y142 phosphorylation drive diabetic angiogenesis via upregulating KDR/HDAC9.

Periodicals：Cell communication and signaling

IF：8.200

Article：Ferroptosis inhibitor alleviates sorafenib-induced cardiotoxicity by attenuating KLF11-mediated FSP1-dependent ferroptosis.

Periodicals：International journal of biological sciences

IF：8.200

Article：CEBPB-mediated upregulation of SERPINA1 promotes colorectal cancer progression by enhancing STAT3 signaling. 

Periodicals：Cell death discovery

IF：6.100

Article：Long non-coding RNA LncTUG1 regulates favourable compression force-induced cementocytes mineralization via PU.1/TLR4/SphK1 signalling. 

Periodicals：Cell Proliferaiton

IF：5.900

Article：FOXC1 transcriptionally suppresses ABHD5 to inhibit the progression of renal cell carcinoma through AMPK/mTOR pathway.

Periodicals：Cell Biology and Toxicology

IF：5.300

Article：YTHDF2-mediated circYAP1 drives immune escape and cancer progression through activating YAP1/TCF4-PD-L1 axis. 

Periodicals：iSence

IF：4.600

Article：GDF15 enhances anoikis resistance and metastasis of gastric cancer through protective autophagy. 

Periodicals：Cellular signalling

IF：4.400

Article：Long noncoding RNA DANCR aggravates breast cancer through the miR34c/E2F1 feedback loop. 

Periodicals：Molecular medicine reports

IF：3.400

【2023年】

Article：Activation of Piezo1 promotes osteogenic differentiation of aortic valve interstitial cell through YAP-dependent glutaminolysis

Periodicals：Science Advances

IF：13.600

Article：Low expression of m6A reader YTHDC1 promotes progression of ovarian cancer via PIK3R1/STAT3/GANAB axis

Periodicals：International Journal of Biological Sciences

IF：9.200

Article：Melatonin suppresses tumor proliferation and metastasis by targeting GATA2 in endometrial cancer.

Periodicals：Journal of pineal research

IF：8.300

Article：Identification of a RAD51B enhancer variant for susceptibility and progression to glioma

Periodicals：Cancer Cell International

IF：5.800

Article：LncRNA WAC-AS1 promotes osteosarcoma Metastasis and stemness by sponging miR-5047 to upregulate SOX2.

Periodicals：Biology direct

IF：5.700

Article：Nucleus-localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription

Periodicals：Cancer Science

IF：5.700

Article：LincRNA00612 inhibits apoptosis and inflammation in LPS-induced BEAS-2B cells via enhancing interaction between p-STAT3 and A2M promoter

Periodicals：PeerJ

IF：2.700

【2022年】

Article：STAT3-NAV2 axis as a new therapeutic target for rheumatoid arthritis via activating SSH1L/Cofilin-1 signaling pathway

Periodicals：Signal Transduction and Targeted Therapy

IF：39.300

Article：N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer

Periodicals：Nature Communications

IF：16.600

Article：Dual immunological and proliferative regulation of immune checkpoint FGL1 in lung adenocarcinoma: The pivotal role of the YY1–FGL1–MYH9 axis

Periodicals：Frontiers in Immunology

IF：7.300

Article：Interaction between endogenous microRNAs and virus-derived small RNAs controls viral replication in insect vectors

Periodicals：PLOS Pathogens

IF：6.700

Article：Sp1 S-Sulfhydration Induced by Hydrogen Sulfide Inhibits Inflammation via HDAC6/MyD88/NF-κB Signaling Pathway in Adjuvant-Induced Arthritis

Periodicals：Antioxidants

IF：6.313

Article：Knockdown of YY1 Inhibits XIST Expression and Enhances Cloned Pig Embryo Development

Periodicals：International Journal of Molecular Sciences

IF：6.208

Article：ARID1A downregulation promotes cell proliferation and migration of colon cancer via VIM activation and CDH1 suppression

Periodicals：Journal of Cellular and Molecular Medicine

IF：5.295

【2021年】

Article：Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells

Periodicals：Science Advances

IF：13.600

Article：LncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity

Periodicals：Cell Death & Disease

IF：9.000

Article：NAV2 positively modulates inflammatory response of fibroblast-like synoviocytes through activating Wnt/β-catenin signaling pathway in rheumatoid arthritis

Periodicals：Clinical and Translational Medicine

IF：7.919

Article：An insertion variant of MGMT disrupts a STAT1 binding site and confers susceptibility to glioma

Periodicals：Cancer Cell International

IF：5.722

【2020年】

Article：SLC2A3 promotes macrophage infltration by glycolysis reprogramming in gastric cancer

Periodicals：Cancer Cell International

IF：4.175

【2019年】

Article：Nrf2-regulated miR-380-3p blocks the translation of Sp3 protein and its mediation of paraquat-induced toxicity in mouse neuroblastoma N2a cells

Periodicals：Toxicological Sciences

IF：3.829

Article：Shortened nuclear matrix attachment regions are sufficient for replication and maintenance of episomes in mammalian cells

Periodicals：Molecular Biology of the Cell

IF：3.791

Article：Genetic Predisposition to Glioma Mediated by a MAPKAP1 Enhancer Variant

Periodicals：Cellular and Molecular Neurobiology

IF：3.606

【2018年】

Article：RIF1 promotes human epithelial ovarian cancer growth and progression via activating human telomerase reverse transcriptase expression

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：7.067

【2017年】

Article：LncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1

Periodicals：Journal of Cellular and Molecular Medicine

IF：4.302

【2015年】

Article：Transcriptional upregulation of microtubule-associated protein 2 is involved in the protein kinase A-induced decrease in the invasiveness of glioma cells

Periodicals：Neuro-Oncology

IF：9.384