ChIP试剂盒
实验介绍

染色质免疫沉淀(ChIP)是研究体内蛋白质与DNA相互作用的理想技术,是目前研究转录因子(transcription, TF)与启动子(promoter)区域间相互联系的较好方法。ChIP与其他实验技术相结合,可用于不同用途的检测。ChIP-Seq方法特异性地富集与目的蛋白结合的DNA片段,并对富集得到的DNA片段进行高通量测序,从而确定该转录因子与基因组的结合区域。 (本试剂盒仅供科研用途)

免费咨询向专业的顾问免费咨询
详细介绍


实验原理:


在活细胞状态下使细胞内的蛋白质和DNA交联,并用超声波将其随机切断为一定长度范围内的染色质小片段。选择与目的蛋白特异性结合的抗体免疫沉淀DNA-蛋白质复合体,从而特异性地富集与目的蛋白结合的染色体片段。

 


产品优势:


1. 高效及特异的富集效率;阳性和阴性对照的富集比率>120

2. 对下游分析方法的兼容性强,可兼容RT-qPCR、基因芯片和高通量测序等。

3. 优秀的技术服务团队,技术服务高效、全面、迅速,能及时帮助客户尽快解决实验中遇到的问题。




技术流程:



结果实例:




详见产品说明书Bes5001 ChIP(动物)

详见产品说明书Bes5002 ChIP(植物)



文章案例



2023


ArticleActivation of Piezo1 promotes osteogenic differentiation of aortic valve interstitial cell through YAP-dependent glutaminolysis

Periodicals:Science Advances

IF:13.600


ArticleLow expression of m6A reader YTHDC1 promotes progression of ovarian cancer via PIK3R1/STAT3/GANAB axis

Periodicals:International Journal of Biological Sciences

IF:9.200


ArticleIdentification of a RAD51B enhancer variant for susceptibility and progression to glioma

Periodicals:Cancer Cell International

IF:5.800


ArticleNucleus-localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription

Periodicals:Cancer Science

IF:5.700


ArticleLincRNA00612 inhibits apoptosis and inflammation in LPS-induced BEAS-2B cells via enhancing interaction between p-STAT3 and A2M promoter

PeriodicalsPeerJ

IF:2.700



2022


Article:STAT3-NAV2 axis as a new therapeutic target for rheumatoid arthritis via activating SSH1L/Cofilin-1 signaling pathway

PeriodicalsSignal Transduction and Targeted Therapy

IF39.300


ArticleN6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer

PeriodicalsNature Communications

IF16.600


ArticleDual immunological and proliferative regulation of immune checkpoint FGL1 in lung adenocarcinoma: The pivotal role of the YY1FGL1MYH9 axis

PeriodicalsFrontiers in Immunology

IF:7.300


ArticleInteraction between endogenous microRNAs and virus-derived small RNAs controls viral replication in insect vectors

PeriodicalsPLOS Pathogens

IF:6.700


ArticleSp1 S-Sulfhydration Induced by Hydrogen Sulfide Inhibits Inflammation via HDAC6/MyD88/NF-κB Signaling Pathway in Adjuvant-Induced Arthritis

PeriodicalsAntioxidants

IF6.313


ArticleKnockdown of YY1 Inhibits XIST Expression and Enhances Cloned Pig Embryo Development

PeriodicalsInternational Journal of Molecular Sciences

IF6.208


ArticleARID1A downregulation promotes cell proliferation and migration of colon cancer via VIM activation and CDH1 suppression

PeriodicalsJournal of Cellular and Molecular Medicine

IF5.295



2021


ArticleLoss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells

PeriodicalsScience Advances

IF13.600


ArticleLncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity

PeriodicalsCell Death & Disease

IF:9.000


ArticleNAV2 positively modulates inflammatory response of fibroblast-like synoviocytes through activating Wnt/β-catenin signaling pathway in rheumatoid arthritis

PeriodicalsClinical and Translational Medicine

IF7.919


ArticleAn insertion variant of MGMT disrupts a STAT1 binding site and confers susceptibility to glioma

PeriodicalsCancer Cell International

IF5.722



2020


ArticleSLC2A3 promotes macrophage infltration by glycolysis reprogramming in gastric cancer

PeriodicalsCancer Cell International

IF4.175



2019


ArticleNrf2-regulated miR-380-3p blocks the translation of Sp3 protein and its mediation of paraquat-induced toxicity in mouse neuroblastoma N2a cells

PeriodicalsToxicological Sciences

IF3.829


ArticleShortened nuclear matrix attachment regions are sufficient for replication and maintenance of episomes in mammalian cells

PeriodicalsMolecular Biology of the Cell

IF3.791


ArticleGenetic Predisposition to Glioma Mediated by a MAPKAP1 Enhancer Variant

PeriodicalsCellular and Molecular Neurobiology

IF3.606



2018


ArticleRIF1 promotes human epithelial ovarian cancer growth and progression via activating human telomerase reverse transcriptase expression

PeriodicalsJournal of Experimental & Clinical Cancer Research

IF7.067



2017


ArticleLncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1

PeriodicalsJournal of Cellular and Molecular Medicine

IF4.302



2015


ArticleTranscriptional upregulation of microtubule-associated protein 2 is involved in the protein kinase A-induced decrease in the invasiveness of glioma cells

PeriodicalsNeuro-Oncology

IF9.384

上一篇:没有了

下一篇:没有了