实验原理：

TRAP方法首先设计GST-MS2融合表达载体和ncRNA-MS2茎环结构串联重复载体，共转细胞获得GST-MS2融合蛋白和ncRNA-MS2融合RNA，在胞内形成GST-MS2~ncRNA-MS2与蛋白或RNA的复合体；然后裂解细胞，利用谷胱甘肽亲和琼脂糖磁珠拉取获得目的蛋白-RNA复合物；最后进一步分离纯化，获得目的蛋白或目的RNA进行下一步检测分析。

 

产品优势：

1. 伯信独立研发。

2. 解决了传统的TRAP实验目前只应用于LncRNA等互作RNA或蛋白研究的局限性问题。

3. 使用环状RNA表达载体而非探针进行杂交，避免了circRNA研究中接头序列被蛋白结合后探针不能结合circRNA导致的假阴性影响，且降低了探针杂交过程中RNA的降解。

技术流程：

结果实例

详见产品说明书：Bes5106 TRAP 

文章案例

Article：Circ6834 suppresses non-small cell lung cancer progression by destabilizing ANHAK and regulating miR-873-5p/TXNIP axis.

Periodicals：Molecular Cancer

IF：27.700

Article：CircNUP50 is a novel therapeutic target that promotes cisplatin resistance in ovarian cancer by modulating p53 ubiquitination.

Periodicals：Journal of nanobiotechnology

IF：10.200

Article：TRMT10C-mediated m7G modification of circFAM126A inhibits lung cancer growth by regulating cellular glycolysis.

Periodicals：Cell Biology and Toxicology

IF：5.300

Article：circEIF3I facilitates the recruitment of SMAD3 to early endosomes to promote TGF-β signalling pathway-mediated activation of MMPs in pancreatic cancer

Periodicals：Molecular Cancer

IF：37.299

Article：LINC02159 promotes non-small cell lung cancer progression via ALYREF/YAP1 signaling

Periodicals：Molecular Cancer

IF：37.299

Article：Circular RNA EIF4G3 suppresses gastric cancer progression through inhibition of β-catenin by promoting δ-catenin ubiquitin degradation and upregulating SIK1

Periodicals：Molecular Cancer

IF：37.299

Article：ALKBH5-mediated m6A modifcation of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.300

Article：Circular RNA circLAMA3 inhibits the proliferation of bladder cancer by directly binding an mRNA

Periodicals：Molecular Therapy: Oncolytics

IF：5.700

Article：Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis

Periodicals：Cell Biology and Toxicology

IF：6.100

Article：CircDIDO1 inhibits gastric cancer progression by encoding a novel DIDO1-529aa protein and regulating PRDX2 protein stability

Periodicals：Molecular Cancer

IF：37.300