实验原理：

在变性条件下将待检的RNA样品进行凝胶电泳，再将位于凝胶特定位置的RNA转移到尼龙膜上，固定后再与同位素或其他标记的RNA探针进行杂交。如果待测样品中含有与探针互补的序列，则两者通过碱基互补配对原理特异性结合，随后，洗涤游离的探针，用显影技术检测膜上待测RNA的条带深浅，进而反映出样本中RNA表达水平。

 

产品优势：

1.配备完整：包含RNA提取试剂，可获得高品质的完整RNA。

2.灵敏度高：检测灵敏度高，检测背景低。

3.个性化服务：可根据需要定制个性化高灵敏度探针。

技术流程：

结果实例：

 

详见产品说明书：Bes3016 Northern Blot Kit（miRNA）

 

文章案例

 

Article： A new anti-CRISPR gene promotes the spread of drug-resistance plasmids in Klebsiella pneumoniae. 

Periodicals：Nucleic Acids Research

IF：16.600

 

Article：LINC00842 inactivates transcription co-regulator PGC-1α to promote pancreatic cancer malignancy through metabolic remodelling

Periodicals：Nature Communications

IF：16.600

Article：N6-methyladenosine–Mediated Upregulation of WTAPP1 Promotes WTAP Translation and Wnt Signaling to Facilitate Pancreatic Cancer Progression

Periodicals：Cancer Research

IF：11.200

Article：Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression

Periodicals：NATURE COMMUNICATIONS

IF：16.600

Article：PIWI-interacting RNA-54265 is oncogenic and a potential therapeutic target in colorectal adenocarcinoma

Periodicals：Theranostics

IF：12.400

Article： LINC01615 maintains cell survival in adaptation to nutrient starvation through the pentose phosphate pathway and modulates chemosensitivity in colorectal cancer.

Periodicals：Cellular and molecular life sciences

IF：6.200