实验介绍：

FISH探针是指能与特定核酸序列发生特异性互补杂交，杂交后又能被特殊方法检测的已知并被标记的核苷酸序列。FISH探针通过标记上荧光染料标记，后续用于可视化目标DNA序列的位置和数量。在确定目的基因序列后，选择与目的基因序列互补的序列片段作为探针序列，并根据实验条件选择合适的荧光标记物进行标记。

探针类型：

①短链/寡核酸探针（15-40bp）是利用荧光染料标记到单链DNA上，通常使用化学合成方法，这可以通过磷酸化修饰或末端标记的方法将荧光染料引入到DNA分子的5' 末端或内部；

②长链探针(150-400bp）是将DNA分子的一端进行荧光染料标记，形成互补链5' 端带有荧光标记的DNA双链，通常使用PCR的方法进行标记将荧光染料引入到DNA分子其中一条链的5' 端。

标记类型：5' 端FAM/CY3/CY5等荧光标记。

规格：30T/50T/100T

文章案例：

Article：circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways. 

Periodicals：Molecular therapy. Nucleic acids

IF：6.500

Article：m6A-modified exosome-derived circHIF1α binding to KH domain of IGF2BP3 mediates DNA damage and arrests G1/S transition phase to resists bacterial infection in bacteremia.

Periodicals：Journal of Nanobiotechnology

IF：10.600

Article：TTBK2 circular RNA promotes glioma malignancy by regulating miR-217/HNF1β/Derlin-1 pathway.

Periodicals：The Clinical Respiratory Journal

IF：29.500

Article：Up-regulation of lncRNA CASC9 promotes esophageal squamous cell carcinoma growth by negatively regulating PDCD4 expression through EZH2.

Periodicals：Molecular cancer

IF：27.700

Article：Long noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1.

Periodicals：Clinical and translational medicine

IF：7.900