实验原理：



RIP技术运用针对目标蛋白的抗体免疫沉淀相应的RNA-蛋白复合物，并纯化与蛋白结合的RNA。最后，结合基因特异性分析技术（PCR、qRT-PCR）或高通量分析技术（高通量测序、基因芯片），分析结合在复合物上的RNA类型及数量。

 

产品优势：



1. 伯信独立研发。

2. 提供优化的磁珠，适于免疫沉淀RNA-蛋白复合物。

3. 高品质，稳定性好，

4. 结果准确，重复性好。

5. 操作简单，快速省时。



技术流程：

结果实例：




详见产品说明书：Bes5101 RIP(12T、24T、40T)

文章案例

【2026年】

Article：TENT5A Maintains MYC mRNA Stability to Enhance Osteosarcoma Stemness

Periodicals：Cancer Research

IF：16.6

 

Article：hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte

Periodicals：Nature Communications

IF：15.7001

 

Article：Astrocytic TIA1-Mediated Stress Granules Promote the Demyelination of Optic Neuritis

Periodicals：Advanced Science

IF：14.1006

 

Article：Cannabidiol triggers fatty acids β-oxidation mediated by Stat2

Periodicals：Experimental & Molecular Medicine

IF：12.9

 

Article：Oxidative stress-driven m5 C methylation by NSUN5 confers cisplatin resistance

Periodicals：Redox Biology

IF：11.9

 

Article：Y chromosome-linked EIF1AY deletion drives sex differencesinmultiplemyeloma

Periodicals：npj Precision Oncology

IF：8.000

 

Article：DKC1 a robust prognostic factor and potential therapeutic target in multiple myeloma

Periodicals：Journal of Translational Medicine

IF：7.4996

 

Article：NAT10 promotes gallbladder cancer progression by remodeling cholesterol metabolism

Periodicals：Cell Death Discovery

IF：7.0

【2024年】




Article：Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway.

Periodicals：Molecular cancer

IF：27.700



Article：Natural product mediated mesenchymal-epithelial remodeling by covalently binding ENO1 to degrade m6A modified β-catenin mRNA.

Periodicals：Acta Pharmaceutica Sinica. B

IF：14.700



Article：Parental experiences orchestrate locust egg hatching synchrony by regulating nuclear export of precursor miRNA.

Periodicals：Nature communications

IF：14.700




Article： PTBP3 Mediates IL-18 Exon Skipping to Promote Immune Escape in Gallbladder Cancer.

Periodicals：Advanced science

IF：14.300



Article： Targeting piRNA-137463 Inhibits Tumor Progression and Boosts Sensitivity to Immune Checkpoint Blockade via De Novo Cholesterol Biosynthesis in Lung Adenocarcinoma. 

Periodicals：Advanced science (Weinheim, Baden-Wurttemburg, Germany)

IF：14.100



Article：LncRNA BCAN-AS1 stabilizes c-Myc via N6-methyladenosine-mediated binding with SNIP1 to promote pancreatic cancer.

Periodicals：Cell death and diferentiation

IF：13.700



Article：Cold-inducible RNA binding protein alleviates iron overload-induced neural ferroptosis under perinatal hypoxia insult.
Periodicals：Cell death and diferentiation

IF：13.700



Article：Positive feedback loop of c-myc/XTP6/NDH2/NF-κB to promote malignant progression in glioblastoma. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400




Article：Targeting YTHDF2 inhibits tumorigenesis of diffuse large B-cell lymphoma through ACER2-mediated ceramide catabolism.

Periodicals：Journal of Advanced Research

IF：11.400




Article：FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400



Article：Histone lactylation-regulated METTL3 promotes ferroptosis via m6A-modification on ACSL4 in sepsis-associated lung injury. 

Periodicals：Redox biology

IF：10.700



Article：M6A demethylase FTO-stabilized exosomal circBRCA1 alleviates oxidative stress-induced granulosa cell damage via the miR-642a-5p/FOXO1 axis. 

Periodicals：Journal of nanobiotechnology

IF：10.600



Article：CircNUP50 is a novel therapeutic target that promotes cisplatin resistance in ovarian cancer by modulating p53 ubiquitination.

Periodicals：Journal of nanobiotechnology

IF：10.600




Article：Manganese induces podocyte injury through regulating MTDH/ALKBH5/NLRP10 axis: Combined analysis at epidemiology and molecular biology levels. 

Periodicals：Environment International

IF：10.300



Article：m6A-methylase METTL3 promotes retinal angiogenesis through modulation of metabolic reprogramming in RPE cells. 

Periodicals：Journal of Neuroinflammation

IF：9.300




Article： RUNX1, FUS, and ELAVL1-induced circPTPN22 promote gastric cancer cell proliferation, migration, and invasion through miR-6788-5p/PAK1 axis-mediated autophagy. 

Periodicals：Cellular & Molecular Biology Letters

IF：9.200




Article：NONO promotes gallbladder cancer cell proliferation by enhancing oncogenic RNA splicing of DLG1 through interaction with IGF2BP3/RBM14. 

Periodicals：Cancer Letters

IF：9.100



Article：CircPCNXL2 promotes papillary thyroid carcinoma progression by regulating fatty acid metabolism induced by anabolic enzyme ACC1. 

Periodicals：Cancer Letters

IF：9.100



Article：STAT6/LINC01637 axis regulates tumor growth via autophagy and pharmacological targeting STAT6 as a novel strategy for uveal melanoma. 

Periodicals：Cell death & disease

IF：8.100



Article：NPAS2 dampens chemo-sensitivity of lung adenocarcinoma cells by enhancing DNA damage repair.

Periodicals：Cell death & disease

IF：8.100



Article：Regulation of ULK1 by WTAP/IGF2BP3 axis enhances mitophagy and progression in epithelial ovarian cancer. 

Periodicals：Cell death & disease

IF：8.100




Article：The m6A reader IGF2BP2 regulates glycolytic metabolism and mediates histone lactylation to enhance hepatic stellate cell activation and liver fibrosis. 

Periodicals：Cell death & disease

IF：8.100



Article：N6-methyladenosine-mediated upregulation of LNCAROD confers radioresistance in esophageal squamous cell carcinoma through stabilizing PARP1. 

Periodicals：Clinical and Translational Medicine

IF：7.900



Article：ALKBH5 targets ACSL4 mRNA stability to modulate ferroptosis in hyperbilirubinemia-induced brain damage.

Periodicals：Free Radical Biology & Medicine

IF：7.100




【2023年】




Article：From tuberculosis bedside to bench: UBE2B splicing as a potential biomarker and its regulatory mechanism

Periodicals：Signal Transduction and Targeted Therapy

IF：39.3



Article：CircRREB1 mediates lipid metabolism related senescent phenotypes in chondrocytes through FASN post-translational modifications

Periodicals：Nature Communications

IF：16.600



Article：Tumor Cell Derived Lnc-FSD2-31:1 Contributes to Cancer-Associated Fibroblasts Activation in Pancreatic Ductal Adenocarcinoma Progression through Extracellular Vesicles Cargo MiR-4736

Periodicals：Advanced Science

IF：15.100



Article：N4-Acetylcytidine Drives Glycolysis Addiction in Gastric Cancer via NAT10/SEPT9/HIF-1α Positive Feedback Loop

Periodicals：Advanced Science

IF：15.100




Article：ECSIT Is a Critical Factor for Controlling Intestinal Homeostasis and Tumorigenesis through Regulating the Translation of YAP Protein

Periodicals：Advanced Science

IF：15.100



Article：Identification of TMEM53 as a novel SADS-CoV restriction factor that targets viral RNA-dependent RNA polymerase

Periodicals：Emerging Microbes & Infections

IF：13.200




Article：Harnessing endogenous transcription factors directly by small molecules for chemically induced pluripotency inception

Periodicals：PNAS

IF：11.100




Article：M6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy

Periodicals：International Journal of Biological Sciences

IF：10.750



Article：Intervening in hnRNPA2B1-mediated exosomal transfer of tumor-suppressive miR-184-3p for tumor microenvironment regulation and cancer therapy. J Nanobiotechnology. 

Periodicals：Journal of nanobiotechnology

IF：10.600



Article：Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration

Periodicals：Clinical and Translational Medicine

IF：10.600



Article：Identification of a novel heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) ligand that disrupts HnRNPA2B1/nucleic acid interactions to inhibit the MDMX-p53 axis in gastric cancer

Periodicals：Pharmacological Research

IF：10.334



Article：CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling


Periodicals：Translational Research

IF：10.17



Article：WTAP-mediated m6A modification modulates bone marrow mesenchymal stem cells differentiation potential and osteoporosis

Periodicals：Cell Death and Disease

IF：9.697



Article：METTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer

Periodicals：Cell Death and Disease

IF：9.696



Article：Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis

Periodicals：BMC Medicine

IF：9.300



Article： Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis

Periodicals：International Journal of Biological Sciences

IF：9.200



Article： N4-acetylcytidine-dependent GLMP mRNA stabilization by NAT10 promotes head and neck squamous cell carcinoma metastasis and remodels tumor microenvironment through MAPK/ERK signaling pathway

Periodicals：Cell Death & Disease

IF：9.000



Article：CircZSWIM6 mediates dysregulation of ECM and energy homeostasis in ageing chondrocytes through RPS14 post-translational modification

Periodicals：Clinical and Translational Medicine

IF：8.533



Article：FTO facilitates cancer metastasis by modifying the m6A level of FAP to induce integrin/FAK signaling in non-small cell lung cance

Periodicals：Cell Communication and Signaling

IF：8.400



Article：Human Keratinocyte-Derived Exosomal MALAT1 Promotes Diabetic Wound Healing by Upregulating MFGE8 via microRNA-1914-3p

Periodicals：International Journal of Nanomedicine

IF：7.033



Article：A novel circular RNA, circMAML3, promotes tumor progression of prostate cancer by regulating miR-665/MAPK8IP2 axis.

Periodicals：Cell Death Discovery

IF：7.000



Article：METTL3-mediated m6A methylation of C1qA regulates the Rituximab resistance of diffuse large B-cell lymphoma cells.

Periodicals：Cell Death Discovery

IF：7.000



【2022年】



Article：The short inverted repeats-induced circEXOC6B inhibits prostate cancer metastasis by enhancing the binding of RBMS1 and HuR
Periodicals：Molecular Therapy

IF：12.91



Article：STAT3/miR-130b-3p/MBNL1 feedback loop regulated by mTORC1 signaling promotes angiogenesis and tumor growth


Periodicals：Journal of Experimental & Clinical Cancer Research

IF：12.658



Article：ALKBH5-mediated m6A modifcation of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：12.658



Article：PHGDH Inhibits Ferroptosis and Promotes Malignant Progression by Upregulating SLC7A11 in Bladder Cancer

Periodicals：International Journal of Biological Sciences

IF：10.75



Article：Novel roles of LSECtin in gastric cancer cell adhesion, migration, invasion, and lymphatic metastasis

Periodicals：Cell Death and Disease

IF：9.685



Article：Hotair promotes the migration and proliferation in ovarian cancer by miR‑222‑3p/CDK19 axis

Periodicals：Cellular and Molecular Life Sciences

IF：9.26



Article：Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer

Periodicals：Cancer Letters

IF：8.697



Article：circRanGAP1/miR-27b-3p/NRAS Axis may promote the progression of hepatocellular Carcinoma

Periodicals：Experimental Hematology & Oncology

IF：8.593



Article：YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis

Periodicals：Cell Death & Disease

IF：8.469



Article：circCRKL, a circRNA derived from CRKL,regulates BCR-ABL via sponging miR-877-5p to promote chronic myeloid leukemia cell proliferation

Periodicals：Journal of Translational Medicine

IF：8.439




Article：PRP8-Induced CircMaml2 Facilitates the Healing of the Intestinal Mucosa via Recruiting PTBP1 and Regulating Sec62

Periodicals：Cells

IF：7.666



Article：Interaction between endogenous microRNAs and virus-derived small RNAs controls viral replication in insect vectors

Periodicals：PLOS Pathogens

IF：7.455




【2021年】




Article：NAT10 promotes gastric cancer metastasis via N4-acetylated COL5A1

Periodicals：Signal Transduction and Targeted Therapy

IF：13.493



Article：Downregulation of microRNA-6125 promotes colorectal cancer growth through YTHDF2-dependent recognition of N6-methyladenosine-modified GSK3β


Periodicals：Clinical and Translational Medicine

IF：11.492



Article：LINC00473-modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid-induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation

Periodicals：Bioengineering & Translational Medicine

IF：10.711



Article：HnRNP-L-regulated circCSPP1/miR-520h/EGR1 axis modulates autophagy and promotes progression in prostate cancer

Periodicals：Molecular Therapy-Nucleic Acids

IF：8.886



Article：hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3

Periodicals：Molecular Therapy-Nucleic Acids

IF：8.886



Article：CircHAS2 promotes the proliferation, migration, and invasion of gastric cancer cells by regulating PPM1E mediated by hsa-miR-944

Periodicals：Cell Death & Disease

IF：8.469



Article：LncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity

Periodicals：Cell Death & Disease

IF：8.469



Article：LINC-PINT impedes DNA repair and enhances radiotherapeutic response by targeting DNA-PKcs in nasopharyngeal cancer

Periodicals：Cell Death & Disease

IF：8.469



Article：LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma

Periodicals：Cell Death & Disease

IF：8.469