实验原理：

ChIRP方法通过设计生物素标记RNA互补探针，并使其与链霉亲和素结合，这样，在探针与目标RNA特异性结合的同时，捕获RNA结合调控的DNA染色体片段与参与转录调控的RNA结合蛋白质（RBPs）。

DNA染色体片段经过文库构建与高通量测序，在基因组水平上获得转录调控RNA（增强子RNA, eRNA）调控的下游靶基因，结合qPCR可以研究结合调控强度； RNA结合蛋白经过酶消化与高效液相-质谱分析，可以鉴定参与转录调控的蛋白质，结合Western Blot可以进一步研究结合作用强度。

 

产品优势：

1. 伯信独立研发，具有自主知识产权。

2. 特异性和灵敏度高，稳定性好。

3. 检测方法领先，结果准确、重复性好。

4. 快速检测，操作简单，安全便捷。

技术路线：

详见产品说明书：Bes5104-1 ChIRP-DNA

详见产品说明书：Bes5104-2 ChIRP-Protein

详见产品说明书：Bes5104-3 ChIRP-DNA、Protein

注：Bes5104-3 ChIRP-DNA&Protein （同时拉RNA结合DNA和蛋白）的用户可根据实验目的自行参考说明书Bes5104-1 ChIRP-DNA（只研究RNA结合DNA） 或Bes5104-2 ChIRP-Protein （只研究RNA结合蛋白）进行实验操作。 

文章案例

【2024年】

Article： Genome-wide enhancer RNA profiling adds molecular links between genetic variation and human cancers.

Periodicals：Military Medical Research

IF：16.700

Article： LINC00606 promotes glioblastoma progression through sponge miR-486-3p and interaction with ATP11B. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400

Article：Positive feedback loop of c-myc/XTP6/NDH2/NF-κB to promote malignant progression in glioblastoma. 

Periodicals：Journal of experimental & clinical cancer research

IF：11.400

Article：M6A-mediated upregulation of lncRNA TUG1 in liver cancer cells regulates the antitumor response of CD8+ T cells and phagocytosis of macrophages. 

Periodicals：Advanced Science

IF：14.300

Article： CircARAP2 controls sMICA-induced NK cell desensitization by erasing CTCF/PRC2-induced suppression in early endosome marker RAB5A.

Periodicals：Cellular and Molecular Life Sciences

IF：6.200

【2023年】

Article：hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma.

Periodicals：Molecular Cancer

IF：37.2999

Article：M6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy

Periodicals：International Journal of Biological Sciences

IF：9.200

Article：Long Non-Coding RNA BNIP3 Inhibited the Proliferation of Bovine Intramuscular Preadipocytes via Cell Cycle

Periodicals：International Journal of Molecular Sciences

IF：5.600

Article：Lnc-LRRTM4 promotes proliferation, metastasis and EMT of colorectal cancer through activating LRRTM4 transcription

Periodicals：Cancer Cell International

IF：5.800

【2022年】

Article：5mC and H3K9me3 of TRAF3IP2 promoter region accelerates the progression of translocation renal cell carcinoma

Periodicals：Biomarker Research

IF：11.100

Article：LncRNA HOXA11-AS promotes glioma malignant phenotypes and reduces its sensitivity to ROS via Tpl2-MEK1/2-ERK1/2 pathway

Periodicals：Cell Death and Disease

IF：9.000

Article： LINC01615 maintains cell survival in adaptation to nutrient starvation through the pentose phosphate pathway and modulates chemosensitivity in colorectal cancer.

Periodicals：Cellular and molecular life sciences

IF：6.200

Article：LncRNA FENDRR with m6A RNA methylation regulates hypoxia-induced pulmonary artery endothelial cell pyroptosis by mediating DRP1 DNA methylation

Periodicals：Molecular Medicine

IF：5.700

【2021年】

Article：Downregulation of microRNA-6125 promotes colorectal cancer growth through YTHDF2-dependent recognition of N6-methyladenosine-modified GSK3β

Periodicals：Clinical and Translational Medicine

IF：10.600

Article：Upregulated LINC01667 Expression Is Correlated With Poor Prognosis in Hepatocellular Carcinoma

Periodicals：Frontiers in Oncology

IF：6.244

【2020年】

Article：Long Noncoding RNA MRPL23-AS1 Promotes Adenoid Cystic Carcinoma Lung Metastasis

Periodicals：Cancer Research

IF：11.200

Article：Super-Enhancer LncRNA LINC00162 Promotes Progression of Bladder Cancer

Periodicals：iScience

IF：4.447

【2019年】

Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

Periodicals：Advanced Science

IF：17.521

【2017年】

Article：LncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1

Periodicals：Journal of Cellular and Molecular Medicine

IF：4.302