ChIRP试剂盒
实验介绍

ChIRP(Chromatin Isolation by RNA Purification)是一项研究RNA与DNA及蛋白质之间相互作用的技术。根据研究对象不同,ChIRP技术可分别结合高通量测序(ChIRP-Seq)和质谱技术(ChIRP-MS),研究与目标RNA互作的基因和蛋白质。 (本试剂盒仅供科研用途)

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详细介绍


实验原理:


ChIRP方法通过设计生物素标记RNA互补探针,并使其与链霉亲和素结合,这样,在探针与目标RNA特异性结合的同时,捕获RNA结合调控的DNA染色体片段与参与转录调控的RNA结合蛋白质(RBPs)。


DNA染色体片段经过文库构建与高通量测序,在基因组水平上获得转录调控RNA(增强子RNA, eRNA)调控的下游靶基因,结合qPCR可以研究结合调控强度; RNA结合蛋白经过酶消化与高效液相-质谱分析,可以鉴定参与转录调控的蛋白质,结合Western Blot可以进一步研究结合作用强度。

 

产品优势:


1. 伯信独立研发,具有自主知识产权。

2. 特异性和灵敏度高,稳定性好。

3. 检测方法领先,结果准确、重复性好。

4. 快速检测,操作简单,安全便捷。


技术路线:






详见产品说明书Bes5104-1 ChIRP-DNA

详见产品说明书Bes5104-2 ChIRP-Protein

详见产品说明书Bes5104-3 ChIRP-DNAProtein

注:Bes5104-3 ChIRP-DNA&Protein (同时拉RNA结合DNA和蛋白)的用户可根据实验目的自行参考说明书Bes5104-1 ChIRP-DNA(只研究RNA结合DNA) 或Bes5104-2 ChIRP-Protein (只研究RNA结合蛋白)进行实验操作。 


文章案例


2023


Articlehsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma.

PeriodicalsMolecular Cancer

IF:37.2999


ArticleM6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy

PeriodicalsInternational Journal of Biological Sciences

IF:9.200


ArticleLong Non-Coding RNA BNIP3 Inhibited the Proliferation of Bovine Intramuscular Preadipocytes via Cell Cycle

PeriodicalsInternational Journal of Molecular Sciences

IF:5.600


ArticleLnc-LRRTM4 promotes proliferation, metastasis and EMT of colorectal cancer through activating LRRTM4 transcription

Periodicals:Cancer Cell International

IF:5.800



2022


Article5mC and H3K9me3 of TRAF3IP2 promoter region accelerates the progression of translocation renal cell carcinoma

PeriodicalsBiomarker Research

IF:11.100


ArticleLncRNA HOXA11-AS promotes glioma malignant phenotypes and reduces its sensitivity to ROS via Tpl2-MEK1/2-ERK1/2 pathway

PeriodicalsCell Death and Disease

IF:9.000


ArticleLncRNA FENDRR with m6A RNA methylation regulates hypoxia-induced pulmonary artery endothelial cell pyroptosis by mediating DRP1 DNA methylation

PeriodicalsMolecular Medicine

IF:5.700



2021


ArticleDownregulation of microRNA-6125 promotes colorectal cancer growth through YTHDF2-dependent recognition of N6-methyladenosine-modified GSK3β

PeriodicalsClinical and Translational Medicine

IF10.600


ArticleUpregulated LINC01667 Expression Is Correlated With Poor Prognosis in Hepatocellular Carcinoma

PeriodicalsFrontiers in Oncology

IF6.244



2020


ArticleLong Noncoding RNA MRPL23-AS1 Promotes Adenoid Cystic Carcinoma Lung Metastasis

PeriodicalsCancer Research

IF11.200


ArticleSuper-Enhancer LncRNA LINC00162 Promotes Progression of Bladder Cancer

PeriodicalsiScience

IF4.447



2019


ArticleCircular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

PeriodicalsAdvanced Science

IF17.521



2017


ArticleLncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1

PeriodicalsJournal of Cellular and Molecular Medicine

IF4.302

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