实验原理：

MeDIP先通过5-甲基胞嘧啶（m5C）抗体富集高甲基化的DNA片段，再根据实验目的采用相应的下游检测技术。

MeDIP-Seq测序是对MeDIP富集所得DNA片段进行高通量测序的全基因组甲基化检测技术，可以在全基因组水平上对CpG密集的高甲基化区域进行高精度研究。

MeDIP-qPCR利用实时定量PCR技术检测MeDIP富集所得DNA片段，再经过数据分析得知待测生物样品中特定位点的甲基化水平。

产品优势：

1. 灵敏性高：待测DNA中5-甲基胞嘧啶分布极不均衡也可检测。

2. 转化效率高：3小时内完成富含GC的DNA的完全转化。

3. 配备完整：该试剂盒包含保护剂，最大限度地避免DNA降解；优化的缓冲液体系能最大限度地提高处理后DNA的回收效率。

4. 应用广泛：适用于各种样本类型。

技术流程：

详见产品说明书：Bes5202-1 MeDIP

详见产品说明书：Bes5202-2 MeDIP（含抗体）

文章案例

 

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