RAP试剂盒
实验介绍

RNA反义纯化技术(RNA Antisense Purification,RAP)运用于研究RNA与DNA、RNA及蛋白质之间相互作用的实验。根据研究对象不同,RAP技术结合了高通量测序(RAP-Seq)和质谱技术(RAP-MS)分别研究与目标RNA互作的基因和蛋白质。 (本试剂盒仅供科研用途)

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详细介绍

       RNA反义纯化技术RNA Antisense PurificationRAP)运用于研究RNADNARNA及蛋白质之间相互作用的实验。根据研究对象不同,RAP技术结合了高通量测序(RAP-Seq)和质谱技术(RAP-MS)分别研究与目标RNA互作的基因和蛋白质。

RAP技术是通过设计生物素或链霉亲和素探针组,把目标RNA拉下来以后,与其共同作用的DNARNA或蛋白质(RBPs)就会附在到磁珠上,最后把基因做高通量测序,这样会得到该调控RNA转录调控的下游靶基因。与此同时,RBPs可以开展Western Blot验证或者MS鉴定未知蛋白。


产品优势

1. 检验灵敏度高,稳定性好。

2. 领先的检测方法、结果准确、重复性好。

3. 广州伯信生物科技有限公司独家研制,具有自主知识产权。


技术路线






详见产品说明书Bes5103-1 RAP-RNA

详见产品说明书Bes5103-2 RAP-Protein

详见产品说明书Bes5103-3 RAP-RNA Protein






文章案例


ArticleThe N6-methyladenosine modifcation of circALG1 promotes the metastasis of colorectal cancer mediated by the miR-342-5p/PGF signalling pathway

PeriodicalsMolecular Cancer

IF27.401


Article:EIF4A3-induced circTOLLIP promotes the progression of hepatocellular carcinoma via the miR-516a-5p/PBX3/EMT pathway

Periodicals:Journal of Experimental & Clinical Cancer Research

IF:11.16


ArticleLINC00473-modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid-induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation

PeriodicalsBioengineering & Translational Medicine

IF10.711


ArticleCircular RNA hsa_circ_0043280 inhibits cervical cancer tumor growth and metastasis via miR-203a-3p/PAQR3 axis

PeriodicalsCell Death & Disease

IF8.469


ArticleCircular RNA circACSL1 aggravated myocardial inflammation and myocardial injury by sponging miR-8055 and regulating MAPK14 expression

PeriodicalsCell Death & Disease

IF8.469


ArticleCircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition

PeriodicalsCell Death & Disease

IF8.469


ArticleCircular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression

PeriodicalsCell Death & Disease

IF8.469


ArticleLncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity

PeriodicalsCell Death & Disease

IF8.469


ArticleCircular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

PeriodicalsMolecular Cancer

IF7.776


ArticlecircRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p

PeriodicalsMolecular Therapy: Nucleic Acids

IF7.030


ArticleCircRNA104250 and lncRNAuc001.dgp.1 promote the PM2.5-induced inflammatory response by co-targeting miR-3607-5p in BEAS-2B cells

PeriodicalsEnvironmental Pollution

IF6.792


ArticleThe regulatory network among CircHIPK3, LncGAS5, and miR-495 promotes Th2 differentiation in allergic rhinitis

PeriodicalsCell Death & Disease

IF6.302


ArticleA novel regulatory network among LncRpa, CircRar1, MiR-671 and apoptotic genes promotes lead-induced neuronal cell apoptosis

PeriodicalsArchives of Toxicology

IF5.728


ArticlelncRNA GAS5, as a ceRNA, inhibits the proliferation of diffuse large Bcell lymphoma cells by regulating the miR18a5p/RUNX1 axis

PeriodicalsINTERNATIONAL JOURNAL OF ONCOLOGY

IF5.65


ArticleCircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5

PeriodicalsFrontiers in Molecular Neuroscience

IF5.630


ArticleLong Noncoding Antisense RNA DDIT4AS1 Regulates Meningitic Escherichia coliInduced Neuroinfammation by Promoting DDIT4 mRNA Stability

PeriodicalsMolecular Neurobiology

IF5.59


ArticleA novel regulatory network among LncRpa, CircRar1, MiR671 and apoptotic genes promotes leadinduced neuronal cell apoptosis

PeriodicalsArchives of Toxicology

IF5.262


Articleuc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation

PeriodicalsFrontiers in Oncology

IF4.848


ArticleCircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression

PeriodicalsCancer Cell International

IF4.175


ArticleCircPCMTD1 Acts as the Sponge of miR-224-5p to Promote Glioma Progression

PeriodicalsFrontiers in Oncology

IF4.137


ArticleMechanism of aberrant long non-coding RNA expression in an adriamycin-resistant liver cancer cell strain

PeriodicalsDigestive and Liver Disease

IF4.088


ArticleLong non-coding RNA lncC11orf54-1 modulates neuroinfammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

PeriodicalsMolecular Brain

IF4.04

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