实验原理：

RAP技术通过设计生物素探针组拉取目标RNA，使与其共同作用的RNA或蛋白质（RBPs）富集在磁珠上并被洗脱。最后，通过高通量测序得到该调控RNA转录调控的下游靶基因，同时，也可以通过Western Blot验证RBPs，或通过MS鉴定未知蛋白。

 

产品优势：

1. 伯信独立研发，具有自主知识产权。

2. 灵敏度高，稳定性好。

3. 检测方法领先，结果准确、重复性好。

技术路线：

详见产品说明书：Bes5103-1 RAP-RNA

详见产品说明书：Bes5103-2 RAP-Protein

详见产品说明书：Bes5103-3 RAP-RNA Protein

注：Bes5103-3 RAP-RNA&Protein （同时拉RNA结合RNA和蛋白）的用户可根据实验目的自行参考说明书Bes5103-1 RAP-RNA（只研究RNA结合RNA） 或Bes5103-2 RAP-Protein （只研究RNA结合蛋白）进行实验操作。

文章案例

【2024年】

Article： Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer.

Periodicals：Molecular cancer

IF：27.700

Article：Exosomal long non-coding RNA TRPM2-AS promotes angiogenesis in gallbladder cancer through interacting with PABPC1 to activate NOTCH1 signaling pathway.

Periodicals：Molecular cancer

IF：27.700

Article：Escherichia coli-Induced cGLIS3-Mediated Stress Granules Activate the NF-κB Pathway to Promote Intrahepatic Cholangiocarcinoma Progression. 

Periodicals：Advanced Science

IF：14.300

Article：circMIRIAF aggravates myocardial ischemia-reperfusion injury via targeting miR-544/WDR12 axis.

Periodicals：Redox biology

IF：10.700

Article：Blockade of the lncRNA-PART1-PHB2 axis confers resistance to PARP inhibitor and promotes cellular senescence in ovarian cancer.

Periodicals：Cancer Letters

IF：9.100

Article：CircNUP54 promotes hepatocellular carcinoma progression via facilitating HuR cytoplasmic export and stabilizing BIRC3 mRNA.

Periodicals：Cell death & disease

IF：8.100

Article：circSORBS1 inhibits lung cancer progression by sponging miR-6779-5p and directly binding RUFY3 mRNA. 

Periodicals：Journal of Translational Medicine

IF：6.100

Article：Three-dimensional cultured human umbilical cord mesenchymal stem cells attenuate pulmonary fibrosis by improving the balance of mitochondrial fusion and fission.

Periodicals：Stem Cells Translational Medicine

IF：5.400

Article：circRUNX2.2, highly expressed in Marek's disease tumor tissues, functions in cis to regulate parental gene RUNX2 expression. 

Periodicals：Poultry Science

IF：3.800

【2023年】

Article：N6-methyladenosine-modified circRIMS2 mediates synaptic and memory impairments by activating GluN2B ubiquitination in Alzheimer's disease.

Periodicals：Translational Neurodegeneration

IF：12.600

Article：METTL3/YTHDC1-medicated m6A modification of circRNA3634 regulates the proliferation and differentiation of antler chondrocytes by miR-124486-5-MAPK1 axis. 

Periodicals：Cellular & molecular biology letters

IF：9.200

Article：Circular RNA circ0001955 promotes cervical cancer tumorigenesis and metastasis via the miR-188-3p/NCAPG2 axis

Periodicals：Journal of Translational Medicine

IF：7.400

Article： N6-methyladenosine modification promotes hepatocarcinogenesis through circ-CDYL-enriched and EpCAM-positive liver tumor-initiating exosomes

Periodicals：iScience

IF：5.800

Article：circIFNGR2 regulating ankylosing spondylitis-associated inflammation through macrophage polarization

Periodicals：iScience

IF：5.800

Article：hucMSCs treatment prevents pulmonary fibrosis by reducing circANKRD42-YAP1-mediated mechanical stiffness.

Periodicals：Aging (Albany NY)

IF：5.200

Article：lhCLIP reveals the in vivo RNA-RNA interactions recognized by hnRNPK

Periodicals：PloS Genetics

IF：4.500

Article：LincRNA00612 inhibits apoptosis and inflammation in LPS-induced BEAS-2B cells via enhancing interaction between p-STAT3 and A2M promoter

Periodicals：PeerJ

IF：2.700

【2022年】

Article：The N6-methyladenosine modifcation of circALG1 promotes the metastasis of colorectal cancer mediated by the miR-342-5p/PGF signalling pathway

Periodicals：Molecular Cancer

IF：37.300

Article：EIF4A3-induced circTOLLIP promotes the progression of hepatocellular carcinoma via the miR-516a-5p/PBX3/EMT pathway

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.300

Article：LINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2

Periodicals：Cell Death and Disease

IF：9.000

Article：CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition

Periodicals：Cell Death and Disease

IF：8.469

Article：The pivotal regulatory factor circBRWD1 inhibits arsenic exposure-induced lung cancer occurrence by bindingmRNA and regulating its stability

Periodicals：Molecular Therapy: Oncolytics

IF：6.311

Article：Long Non‑coding Antisense RNA DDIT4‑AS1 Regulates Meningitic Escherichia coli‑Induced Neuroinfammation by Promoting DDIT4 mRNA Stability

Periodicals：Molecular Neurobiology

IF：5.59

Article：Long non-coding RNA lncC11orf54-1 modulates neuroinfammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

Periodicals：Molecular Brain

IF：4.04

【2021年】

Article：LINC00473-modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid-induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation

Periodicals：Bioengineering & Translational Medicine

IF：7.400

Article：Circular RNA hsa_circ_0043280 inhibits cervical cancer tumor growth and metastasis via miR-203a-3p/PAQR3 axis

Periodicals：Cell Death & Disease

IF：8.469

Article：Circular RNA circACSL1 aggravated myocardial inflammation and myocardial injury by sponging miR-8055 and regulating MAPK14 expression

Periodicals：Cell Death & Disease

IF：8.469

Article：CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition

Periodicals：Cell Death & Disease

IF：8.469

Article：Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression

Periodicals：Cell Death & Disease

IF：8.469

Article：LncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity

Periodicals：Cell Death & Disease

IF：8.469

Article：lncRNA GAS5, as a ceRNA, inhibits the proliferation of diffuse large B‑cell lymphoma cells by regulating the miR‑18a‑5p/RUNX1 axis

Periodicals：INTERNATIONAL JOURNAL OF ONCOLOGY

IF：5.65

Article：CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5

Periodicals：Frontiers in Molecular Neuroscience

IF：5.630

Article：uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation

Periodicals：Frontiers in Oncology

IF：4.848

Article：CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression

Periodicals：Cancer Cell International

IF：4.175

Article：lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression

Periodicals：Experimental and Therapeutic Medicine

IF：2.447

【2020年】

Article：circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p

Periodicals：Molecular Therapy: Nucleic Acids

IF：8.800

Article：CircRNA104250 and lncRNAuc001.dgp.1 promote the PM2.5-induced inflammatory response by co-targeting miR-3607-5p in BEAS-2B cells

Periodicals：Environmental Pollution

IF：6.792

Article：The regulatory network among CircHIPK3, LncGAS5, and miR-495 promotes Th2 differentiation in allergic rhinitis

Periodicals：Cell Death & Disease

IF：6.302

【2019年】

Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

Periodicals：Advanced science

IF：15.100

Article：Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

Periodicals：Molecular Cancer

IF：7.776

Article：CircPCMTD1 Acts as the Sponge of miR-224-5p to Promote Glioma Progression

Periodicals：Frontiers in Oncology

IF：4.137

Article：Mechanism of aberrant long non-coding RNA expression in an adriamycin-resistant liver cancer cell strain

Periodicals：Digestive and Liver Disease

IF：4.088

【2016年】

Article：A novel regulatory network among LncRpa, CircRar1, MiR-671 and apoptotic genes promotes lead-induced neuronal cell apoptosis

Periodicals：Archives of Toxicology

IF：5.728