RNA反义纯化技术（RNA Antisense Purification，RAP）运用于研究RNA与DNA、RNA及蛋白质之间相互作用的实验。根据研究对象不同，RAP技术结合了高通量测序（RAP-Seq）和质谱技术（RAP-MS）分别研究与目标RNA互作的基因和蛋白质。

RAP技术是通过设计生物素或链霉亲和素探针组，把目标RNA拉下来以后，与其共同作用的DNA、RNA或蛋白质（RBPs）就会附在到磁珠上，最后把基因做高通量测序，这样会得到该调控RNA转录调控的下游靶基因。与此同时，RBPs可以开展Western Blot验证或者MS鉴定未知蛋白。

产品优势

1. 检验灵敏度高，稳定性好。

2. 领先的检测方法、结果准确、重复性好。

3. 广州伯信生物科技有限公司独家研制，具有自主知识产权。

技术路线

详见产品说明书：Bes5103-1 RAP-RNA

详见产品说明书：Bes5103-2 RAP-Protein

详见产品说明书：Bes5103-3 RAP-RNA Protein

文章案例

Article：The N6-methyladenosine modifcation of circALG1 promotes the metastasis of colorectal cancer mediated by the miR-342-5p/PGF signalling pathway

Periodicals：Molecular Cancer

IF：27.401

Article：EIF4A3-induced circTOLLIP promotes the progression of hepatocellular carcinoma via the miR-516a-5p/PBX3/EMT pathway

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.16

Article：LINC00473-modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid-induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation

Periodicals：Bioengineering & Translational Medicine

IF：10.711

Article：Circular RNA hsa_circ_0043280 inhibits cervical cancer tumor growth and metastasis via miR-203a-3p/PAQR3 axis

Periodicals：Cell Death & Disease

IF：8.469

Article：Circular RNA circACSL1 aggravated myocardial inflammation and myocardial injury by sponging miR-8055 and regulating MAPK14 expression

Periodicals：Cell Death & Disease

IF：8.469

Article：CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition

Periodicals：Cell Death & Disease

IF：8.469

Article：Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression

Periodicals：Cell Death & Disease

IF：8.469

Article：LncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity

Periodicals：Cell Death & Disease

IF：8.469

Article：Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

Periodicals：Molecular Cancer

IF：7.776

Article：circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p

Periodicals：Molecular Therapy: Nucleic Acids

IF：7.030

Article：CircRNA104250 and lncRNAuc001.dgp.1 promote the PM2.5-induced inflammatory response by co-targeting miR-3607-5p in BEAS-2B cells

Periodicals：Environmental Pollution

IF：6.792

Article：The regulatory network among CircHIPK3, LncGAS5, and miR-495 promotes Th2 differentiation in allergic rhinitis

Periodicals：Cell Death & Disease

IF：6.302

Article：A novel regulatory network among LncRpa, CircRar1, MiR-671 and apoptotic genes promotes lead-induced neuronal cell apoptosis

Periodicals：Archives of Toxicology

IF：5.728

Article：lncRNA GAS5, as a ceRNA, inhibits the proliferation of diffuse large B‑cell lymphoma cells by regulating the miR‑18a‑5p/RUNX1 axis

Periodicals：INTERNATIONAL JOURNAL OF ONCOLOGY

IF：5.65

Article：CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5

Periodicals：Frontiers in Molecular Neuroscience

IF：5.630

Article：Long Non‑coding Antisense RNA DDIT4‑AS1 Regulates Meningitic Escherichia coli‑Induced Neuroinfammation by Promoting DDIT4 mRNA Stability

Periodicals：Molecular Neurobiology

IF：5.59

Article：A novel regulatory network among LncRpa, CircRar1, MiR‑671 and apoptotic genes promotes lead‑induced neuronal cell apoptosis

Periodicals：Archives of Toxicology

IF：5.262

Article：uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation

Periodicals：Frontiers in Oncology

IF：4.848

Article：CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression

Periodicals：Cancer Cell International

IF：4.175

Article：CircPCMTD1 Acts as the Sponge of miR-224-5p to Promote Glioma Progression

Periodicals：Frontiers in Oncology

IF：4.137

Article：Mechanism of aberrant long non-coding RNA expression in an adriamycin-resistant liver cancer cell strain

Periodicals：Digestive and Liver Disease

IF：4.088

Article：Long non-coding RNA lncC11orf54-1 modulates neuroinfammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

Periodicals：Molecular Brain

IF：4.04