祝贺我司客户circRNA互作研究在毒理学权威杂志发表
发布时间 :2016-09-13| BY :伯信官网
RAP试剂盒、circRNA及lncRNA过表达载体、FISH探针—专业、精准、迅速—伯信只用事实说话

       祝贺我司客户关于lncRNA、circRNA与miRNA三者互作的机制研究,目前被毒理学最具影响力杂志Archives of Toxicology收录,该杂志影响因子为6.637,其中RAP试剂盒、circRNAlncRNA过表达载体和FISH探针由伯信生物提供,伯信生物将继续以科技创新为企业的发展动力和方向,成为广大科研伙伴的好助手。  


 

Materials and methods

RNA interference and overexpression

LncRNA and circRNA expression was suppressed by siRNA-mediated knockdown. Three different siRNAs were designed and tested for both lncRpa and circRar1. Overexpression vectors for lncRpa and circRar1 were also constructed (BersinBio, Guangzhou, China)

 

FISH

Cells grown on coverslips were fixed with 4 % paraformaldehyde at room temperature for 15 min, washed twice with 0.1 % diethylpyrocarbonate solution and treated with 0.5 % Triton X-100 at room temperature for 5 min. The samples were dehydrated in a graded series of alcohol and air-dried.After adding probe hybridization solution, the samples were mounted, denatured at 73 °C for 3 min, and hybridized in a humid and dark environment at 37 °C for 12–16 h with Cy3-labeled miRNA probe, 6-carboxyfluoresceinlabeled circRNA probe, and Cy5-labeled lncRNA probe(BersinBio).

 

RNA antisense purification (RAP)试剂盒

The RAP kit (BersinBio) was used for this experiment.RAP employs specific biotinylated probes that hybridize to target RNAs (mRNAs or miRNAs); these can then be pulled down, reverse transcribed to cDNA, and identified by qRT-PCR or sequencing.

 

  目前公司拥有种类齐全的基因表达调控试剂盒:ChIPreverse-ChIPEMSARIPRAPDNA pull downRNA pull down、荧光素酶报基因、DNA甲基化、ChIRP试剂盒等。

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  文献链接:http://link.springer.com/article/10.1007%2Fs00204-016-1837-1